1887

Abstract

The taxonomic position of an endophytic actinomycete, strain GKU 157, isolated from the roots of a jambolan plum tree ( L. Skeels) collected at Khao Khitchakut National Park, Chantaburi province, Thailand, was determined using a polyphasic taxonomic approach. 16S rRNA gene sequence analysis revealed that strain GKU 157 belongs to the genus and formed a distinct phyletic line with NBRC 106107 (98.6 % similarity). Strain GKU 157 formed an extensively branched, non-fragmenting substrate mycelium and aerial hyphae that differentiated into hooked to short spiral chains of about 20 non-motile spores with a warty surface. The cell wall contained -diaminopimelic acid and the whole-cell sugars were galactose, glucose, madurose, mannose and ribose. The -acyl type of muramic acid was acetyl. Mycolic acids were absent. The phospholipids included phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylinositol (PI), phosphatidylinositolmannoside (PIM) and two unknown phospholipids (PLs). The major menaquinone was MK-9(H) and the predominant fatty acids were C, iso-C, Cω9, C and 10-methyl C (tuberculostearic acid). The genomic DNA G+C content was 73.1 mol%. A combination of DNA–DNA hybridization results and significant differences from related species in cultural, physiological and chemical characteristics indicated that strain GKU 157 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GKU 157 ( = BCC 70456 = NBRC 110399).

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2015-06-01
2019-10-16
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