1887

Abstract

A strain designated PYM3-14 was isolated from the drinking water network of Budapest (Hungary) and was studied by polyphasic taxonomic methods. The straight-rod-shaped cells stained Gram-negative, were aerobic and non-motile. Phylogenetic analysis of the 16S rRNA gene sequence of strain PYM3-14 revealed a clear affiliation with members of the family within the class . The 16S rRNA gene sequence of strain PYM3-14 showed the closest sequence similarities to CH15-1 (96.2 %), YC6267 (95.2 %) and UASM D (94.4 %). The DNA G+C content of strain PYM3-14, measured by two different methods (52.0 mol% and 55.9 mol%, respectively), was much lower than that of any member of the genus . The predominant fatty acids (>8 %) were iso-C, iso-C, iso-C, iso-Cω9 and Cω7 alcohol. Strain PYM3-14 contained Q-8 as the major ubiquinone and phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylmonomethylethanolamine as the major polar lipids. According to phenotypic and genotypic data strain PYM3-14 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PYM3-14 ( = NCAIM B 02508 = DSM 25526). On the basis of new data obtained in this study, an emended description of the genus is also proposed.

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2015-06-01
2024-12-06
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References

  1. Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W., Lipman D.J. ( 1997;). Gapped blast psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25 33893402. [CrossRef]
    [Google Scholar]
  2. Aslam Z., Park J.H., Kim S.W., Jeon C.O., Chung Y.R. ( 2009;). Arenimonas oryziterrae sp. nov., isolated from a field of rice (Oryza sativa L.) managed under a no-tillage regime, and reclassification of Aspromonas composti as Arenimonas composti comb. nov.. Int J Syst Evol Microbiol 59 29672972. [CrossRef]
    [Google Scholar]
  3. Barrow G.I., Feltham R.K.A. ( 2003). Cowan and Steel's Manual for the Identification of Medical Bacteria., Cambridge: Cambridge University Press;.
    [Google Scholar]
  4. Cashion P., Holder-Franklin M.A., McCully J., Franklin M. ( 1977;). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81 461466. [CrossRef]
    [Google Scholar]
  5. Chen F., Shi Z., Wang G. ( 2012;). Arenimonas metalli sp. nov., isolated from an iron mine. Int J Syst Evol Microbiol 62 17441749. [CrossRef]
    [Google Scholar]
  6. Christensen P., Cook F.D. ( 1978;). Lysobacter, a new genus of nonfruiting, gliding bacteria with a high base ratio. Int J Syst Bacteriol 28 367393. [CrossRef]
    [Google Scholar]
  7. Collins M.D., Pirouz T., Goodfellow M., Minnikin D.E. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100 221230. [CrossRef]
    [Google Scholar]
  8. Embley T.M., Wait R. ( 1994;). Structural lipids of Eubacteria. . In Chemical Methods in Prokaryotic Systematics, pp. 141147. Edited by Goodfellow M., O'Donnell A. G. New York: Wiley;.
    [Google Scholar]
  9. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376. [CrossRef]
    [Google Scholar]
  10. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791. [CrossRef]
    [Google Scholar]
  11. Groth I., Schumann P., Rainey F.A., Martin K., Schuetze B., Augsten K. ( 1997;). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47 11291133. [CrossRef]
    [Google Scholar]
  12. Hall T.A. ( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41 9598.
    [Google Scholar]
  13. Hasegawa T., Takizawa M., Tanida S. ( 1983;). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29 319322. [CrossRef]
    [Google Scholar]
  14. Homonnay Z.G., Makk J., Brumbauer A., Párkány-Simon B., Márialigeti K.M., Tóth E. ( 2008;). Investigations on the bacterial community of two characteristic sites of drinking water network of Budapest. Hid Közl 88 7981 (in Hungarian) .
    [Google Scholar]
  15. Homonnay Z.G., Török G., Makk J., Brumbauer A., Major E., Márialigeti K., Tóth E. ( 2014;). Bacterial communities in the collection and chlorinated distribution sections of a drinking water system in Budapest, Hungary. J Basic Microbiol 54 729738. [CrossRef]
    [Google Scholar]
  16. Hugh R., Leifson E. ( 1953;). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol 66 2426.
    [Google Scholar]
  17. Huy H., Jin L., Lee Y.-K., Lee K.C., Lee J.-S., Yoon J.-H., Ahn C.-Y., Oh H.-M. ( 2013;). Arenimonas daechungensis sp. nov., isolated from the sediment of a eutrophic reservoir. Int J Syst Evol Microbiol 63 484489. [CrossRef]
    [Google Scholar]
  18. Jin L., Kim K.K., Im W.-T., Yang H.-C., Lee S.-T. ( 2007;). Aspromonas composti gen. nov., sp. nov., a novel member of the family Xanthomonadaceae . Int J Syst Evol Microbiol 57 18761880. [CrossRef]
    [Google Scholar]
  19. Jin L., Kim K.K., An K.G., Oh H.M., Lee S.T. ( 2012;). Arenimonas daejeonensis sp. nov., isolated from compost. Int J Syst Evol Microbiol 62 16741678. [CrossRef]
    [Google Scholar]
  20. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721. [CrossRef]
    [Google Scholar]
  21. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111120. [CrossRef]
    [Google Scholar]
  22. Kwon S.-W., Kim B.-Y., Weon H.-Y., Baek Y.K., Go S.-J. ( 2007;). Arenimonas donghaensis gen. nov., sp. nov., isolated from seashore sand. Int J Syst Evol Microbiol 57 954958. [CrossRef]
    [Google Scholar]
  23. Makk J., Homonnay Z.G., Kéki Z., Lejtovicz Z., Márialigeti K., Spröer C., Schumann P., Tóth E.M. ( 2011;). Tahibacter aquaticus gen. nov., sp. nov., a new gammaproteobacterium isolated from the drinking water supply system of Budapest (Hungary). Syst Appl Microbiol 34 110115. [CrossRef]
    [Google Scholar]
  24. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3 208218. [CrossRef]
    [Google Scholar]
  25. Mesbah M., Premachandran U., Whitman W.B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39 159167. [CrossRef]
    [Google Scholar]
  26. Minnikin D.E., Collins M.D., Goodfellow M. ( 1979;). Fatty acid and polar lipid composition in the classification of Cellulomonas Oerskovia and related taxa. J Appl Bacteriol 47 8795. [CrossRef]
    [Google Scholar]
  27. Poindexter J.S. ( 1991;). Dimorphic prosthecate bacteria: the genera Caulobacter Asticcacaulis Hyphomicrobium Pedomicrobium Hyphomonas Thiodendron . . In The Prokaryotes , 2nd edn., pp. 21762196. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;.
    [Google Scholar]
  28. Quast C., Pruesse E., Yilmaz P., Gerken J., Schweer T., Yarza P., Peplies J., Glöckner F.O. ( 2013;). The silva ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res 41 (D1), D590D596. [CrossRef]
    [Google Scholar]
  29. Rzhetsky A., Nei M. ( 1992;). A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 9 945967.
    [Google Scholar]
  30. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425.
    [Google Scholar]
  31. Schildkraut C., Lifson S. ( 1965;). Dependence of the melting temperature of DNA on salt concentration. Biopolymers 3 195208. [CrossRef]
    [Google Scholar]
  32. Schumann P., Pukall R. ( 2013;). The discriminatory power of ribotyping as automatable technique for differentiation of bacteria. Syst Appl Microbiol 36 369375. [CrossRef]
    [Google Scholar]
  33. Stead D.E., Sellwood J.E., Wilson J., Viney I. ( 1992;). Evaluation of a commercial microbial identification system based on fatty acid profiles for rapid, accurate identification of plant pathogenic bacteria. J Appl Bacteriol 72 315321. [CrossRef]
    [Google Scholar]
  34. Tamaoka J., Komagata K. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25 125128. [CrossRef]
    [Google Scholar]
  35. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729. [CrossRef]
    [Google Scholar]
  36. Tóth E.M., Schumann P., Borsodi A.K., Kéki Z., Kovács A.L., Márialigeti K. ( 2008;). Wohlfahrtiimonas chitiniclastica gen. nov., sp. nov., a new gammaproteobacterium isolated from Wohlfahrtia magnifica (Diptera: Sarcophagidae). Int J Syst Evol Microbiol 58 976981. [CrossRef]
    [Google Scholar]
  37. Tóth E.M., Kéki Z., Makk J., Homonnay Z.G., Márialigeti K., Schumann P. ( 2011;). Nocardioides hungaricus sp. nov., isolated from a drinking water supply system. Int J Syst Evol Microbiol 61 549553. [CrossRef]
    [Google Scholar]
  38. Tóth E.M., Vengring A., Homonnay Z.G., Kéki Z., Spröer C., Borsodi A.K., Márialigeti K., Schumann P. ( 2014;). Phreatobacter oligotrophus gen. nov., sp. nov., an alphaproteobacterium isolated from ultrapure water of the water purification system of a power plant. Int J Syst Evol Microbiol 64 839845. [CrossRef]
    [Google Scholar]
  39. Yamada K., Komagata K. ( 1972;). Taxonomic studies on coryneform bacteria. IV. Morphological, cultural, biochemical, and physiological characteristics. J Gen Appl Microbiol 18 399416. [CrossRef]
    [Google Scholar]
  40. Young C.-C., Kämpfer P., Ho M.-J., Busse H.-J., Huber B.E., Arun A.B., Shen F.-T., Lai W.-A., Rekha P.D. ( 2007;). Arenimonas malthae sp. nov., a gammaproteobacterium isolated from an oil-contaminated site. Int J Syst Evol Microbiol 57 27902793. [CrossRef]
    [Google Scholar]
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