1887

Abstract

A yellow-pigmented strain, designated strain 3.5X, was isolated from oil-contaminated saline soil in Gudao, Shandong Province, China, and was characterized taxonomically. The results showed that the isolate was a Gram-stain-negative, aerobic, motile, rod-shaped cell with a polar flagellum. Phylogenetic analysis of 16S rRNA gene sequences revealed the strain belonged to the family in the class and represented an independent taxon separated from other genera. The closest relative of strain 3.5X was DSM 14263 (94 % similarity). The genomic DNA G+C content was 67 mol% by thermal denaturation and 66.3 mol% from genome sequences. The cells mainly consisted of branched fatty acids, with iso-C, iso-C, iso-Cω9 and/or C10-methyl and iso-C as the major fatty acids. The main polar lipids were phosphatidylmonomethylethanolamine, diphosphatidylglycerol and phosphatidylethanolamine. In addition, ubiquinone Q-8 was the major component of the quinone system and the polyamine pattern contained the major compound spermidine plus minor amounts of putrescine and spermine. On the basis of phenotypic, genotypic and phylogenetic distinctiveness, it is proposed that the isolate represents a novel species in a novel genus, namely gen. nov., sp. nov. The type strain is 3.5X ( = NBRC 110685 = KCTC 42351 = CPCC 100614).

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2015-05-01
2019-09-21
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