1887

Abstract

An aerobic, budding, non-pigmented and rosette-forming bacterium was isolated from a littoral wetland of a boreal lake located in Valaam Island, northern Russia, and designated strain P3. Ellipsoidal to pear-shaped cells of this bacterium were covered with crateriform pits and possessed stalks suggesting a planctomycete morphotype. 16S rRNA gene sequence analysis confirmed that strain P3 was a member of the order and belonged to a phylogenetic lineage defined by the genus , with 89 and 86 % sequence similarity to and , respectively. Strain P3 was a mildly acidophilic, mesophilic organism capable of growth at pH values between pH 4.2 and 7.1 (with an optimum at pH 6.0–6.5) and at temperatures between 10 and 30 °C (optimum at 20–28 °C). Most sugars, a number of polysaccharides and several organic acids were the preferred growth substrates. Compared with and , which require NaCl for growth, strain P3 was salt-sensitive and did not develop at NaCl concentrations above 0.5 % (w/v). The major fatty acids were C and Cω7; the cells also contained significant amounts of Cω7 and C. The major intact polar lipids were diacylglycerol--(,,-trimethyl)homoserine (DGTS) lipids; the major neutral lipids were long-chain 1,(ω-1)-diols and C hydrocarbon. The quinone was MK-6, and the G+C content of the DNA was 59.0 mol%. Strain P3 differed from and by cell morphology, substrate utilization pattern and a number of physiological characteristics. Based on these data, the novel isolate should be considered as representing a novel genus and species of planctomycetes, for which the name gen. nov., sp. nov., is proposed. The type strain is P3 ( = DSM 26348 = VKM B-2887).

Funding
This study was supported by the:
  • Russian Academy of Sciences
  • Russian Fund of Basic Research (Award 12-04-00480)
  • Soehngen Institute for Anaerobic Microbiology
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2015-05-01
2021-07-29
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