1887

Abstract

A rod- or coccus-shaped, non-spore-forming actinobacterium, designated strain SC8A-24, was isolated from a soil sample collected from the rhizosphere of on the southern edge of the Taklimakan desert, Xinjiang, China, and examined by a polyphasic approach to clarify its taxonomic position. This actinobacterium was Gram-staining-positive and aerobic. Substrate and aerial mycelia were not observed, and no diffusible pigments were observed on the media tested. Strain SC8A-24 grew optimally without NaCl at 28–30 °C and pH 7.0–8.0. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain SC8A-24 belonged to the genus and shared the highest 16S rRNA gene sequence similarity with CL-Z59 (96.51 %), OS4 (96.43 %) and BX5-10 (96.37 %). The DNA G+C content of strain SC8A-24 was 71 mol%. The cell-wall peptidoglycan contained -2,6-diaminopimelic acid, and MK-8(H) was the predominant menaquinone. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid and an unidentified phospholipid. The major fatty acids were Cω8, 10-methyl C and Cω9. On the basis of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain SC8A-24 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SC8A-24 ( = DSM 26045 = CGMCC 4.7183).

Funding
This study was supported by the:
  • , National Natural Sciences Foundation of China , (Award 81172963 and 81373308)
  • , National Science and Technology Major Project from the Ministry of Science and Technology of China , (Award 2012ZX09301-002-001-018)
  • , Specialized Research Fund for the Doctoral Programme of Higher Education from the Ministry of Education of China , (Award 20111106110032)
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2015-05-01
2020-09-20
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