1887

Abstract

A novel actinomycete strain, designated TRM 45387, was isolated from a saline-alkali soil in Xinjiang Province (40° 22′ N 79° 08′ E), north-west China. The isolate was characterized using a polyphasic approach. 16S rRNA gene sequence analysis indicated that strain TRM 45387 belonged to the genus and was closely related to DSM 44726 (96.59 % 16S rRNA gene sequence similarity). The G+C content of the DNA was 71.26 mol%. The isolate contained -diaminopimelic acid as the diagnostic diamino acid, and xylose, glucose, galactose, arabinose and ribose as the major whole-cell sugars. The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositolmannosides. The predominant menaquinone was MK-10(H). The major fatty acids were iso-C, anteiso-C and anteiso-C. On the basis of the evidence from this polyphasic study, a novel species, sp. nov., is proposed. The type strain of is TRM 45387 ( = CCTCC AA 2014007 = JCM 30184).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000143
2015-05-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/5/1587.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000143&mimeType=html&fmt=ahah

References

  1. Chun J., Goodfellow M.. ( 1995; ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. . Int J Syst Bacteriol 45:, 240–245. [CrossRef] [PubMed]
    [Google Scholar]
  2. Collins M. D.. ( 1985; ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E... London:: Academic Press;.
    [Google Scholar]
  3. Evtushenko L. I., Taptykova S. D., Akimov V. N., Semyonova S. A., Kalakoutskii L. V.. ( 1991; ). Glycomyces tenuis sp. nov.. Int J Syst Bacteriol 41:, 154–157. [CrossRef]
    [Google Scholar]
  4. Felsenstein J.. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  5. Felsenstein J.. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  6. Fitch W. M.. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  7. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N.. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  8. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K.. ( 1997; ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47:, 1129–1133. [CrossRef] [PubMed]
    [Google Scholar]
  9. Gu Q., Zheng W., Huang Y.. ( 2007; ). Glycomyces sambucus sp. nov., an endophytic actinomycete isolated from the stem of Sambucus adnata Wall. . Int J Syst Evol Microbiol 57:, 1995–1998. [CrossRef] [PubMed]
    [Google Scholar]
  10. Guan T. W., Xia Z. F., Xiao J., Wu N., Chen Z. J., Zhang L. L., Zhang X. P.. ( 2011; ). Glycomyces halotolerans sp. nov., a novel actinomycete isolated from a hypersaline habitat in Xinjiang, China. . Antonie van Leeuwenhoek 100:, 137–143. [CrossRef] [PubMed]
    [Google Scholar]
  11. Han X. X., Luo X. X., Zhang L. L.. ( 2014; ). Glycomyces fuscus sp. nov. and Glycomyces albus sp. nov., actinomycetes isolated from a hypersaline habitat. . Int J Syst Evol Microbiol 64:, 2437–2441. [CrossRef] [PubMed]
    [Google Scholar]
  12. Hasegawa T., Takizawa M., Tanida S.. ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Appl Microbiol 29:, 319–322. [CrossRef]
    [Google Scholar]
  13. Kelly K. L.. ( 1964; ). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  15. Labeda D. P., Kroppenstedt R. M.. ( 2004; ). Emended description of the genus Glycomyces and description of Glycomyces algeriensis sp. nov., Glycomyces arizonensis sp. nov. and Glycomyces lechevalierae sp. nov.. Int J Syst Evol Microbiol 54:, 2343–2346. [CrossRef] [PubMed]
    [Google Scholar]
  16. Labeda D. P., Testa R. T., Lechevalier M. P., Lechevalier H. A.. ( 1985; ). Glycomyces, a new genus of the Actinomycetales. . Int J Syst Bacteriol 35:, 417–421. [CrossRef]
    [Google Scholar]
  17. Labeda D. P., Goodfellow M., Brown R., Ward A. C., Lanoot B., Vanncanneyt M., Swings J., Kim S.-B., Liu Z. et al. ( 2012; ). Phylogenetic study of the species within the family Streptomycetaceae. . Antonie van Leeuwenhoek 101:, 73–104. [CrossRef] [PubMed]
    [Google Scholar]
  18. Minnikin D. E., Alshamaony L., Goodfellow M.. ( 1975; ). Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. . J Gen Microbiol 88:, 200–204. [CrossRef] [PubMed]
    [Google Scholar]
  19. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  20. Qin S., Wang H.-B., Chen H.-H., Zhang Y.-Q., Jiang C.-L., Xu L.-H., Li W.-J.. ( 2008; ). Glycomyces endophyticus sp. nov., an endophytic actinomycete isolated from the root of Carex baccans Nees. . Int J Syst Evol Microbiol 58:, 2525–2528. [CrossRef] [PubMed]
    [Google Scholar]
  21. Qin S., Chen H.-H., Klenk H. P., Zhao G.-Z., Li J., Xu L.-H., Li W.-J.. ( 2009; ). Glycomyces scopariae sp. nov. and Glycomyces mayteni sp. nov., isolated from medicinal plants in China. . Int J Syst Evol Microbiol 59:, 1023–1027. [CrossRef] [PubMed]
    [Google Scholar]
  22. Saitou N., Nei M.. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  23. Sasser M.. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. . USFCC Newsl 20:, 16.
    [Google Scholar]
  24. Shirling E. B., Gottlieb D.. ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  25. Staneck J. L., Roberts G. D.. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  26. Tamaoka J., Komagata K.. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  27. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  28. Xing K., Qin S., Zhang W.-D., Cao C.-L., Ruan J.-S., Huang Y., Jiang J.-H.. ( 2014; ). Glycomyces phytohabitans sp. nov., a novel endophytic actinomycete isolated from the coastal halophyte in Jiangsu, East China. . J Antibiot (Tokyo) 67:, 559–563. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000143
Loading
/content/journal/ijsem/10.1099/ijs.0.000143
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error