1887

Abstract

A novel psychrophilic strain, SSB001, was isolated from an alpine swamp meadow soil in Tibet, China, and identified as a representative of a novel phylogenetic subclade in the genus , with (96.2 %), (96.53 %) and (96.2 %) as the most closely related species on the basis of 16S rRNA gene sequence analyses. The strain was distinguished from defined species of the genus by further study of gene sequences, phenotypic characterization, cellular fatty acid composition, quinones, polar lipids and -diaminopimelic acid in the peptidoglycan. Based upon these results, we propose the strain as a representative of a novel species named sp. nov., with SSB001 ( = ACCC 19728 = DSM 29321) as the type strain. The DNA G+C content (mol%) of strain SSB001 was 40.18 mol% (HPLC).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41301265 and 51221892)
  • Ministry of Science and Technology of China (Award 2013CB956300)
  • STSN
  • -21-02
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2015-05-01
2021-10-27
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References

  1. Ash C., Priest F. G., Collins M. D. ( 1993 ). Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus . . Antonie van Leeuwenhoek 64, 253260. [View Article] [PubMed]
    [Google Scholar]
  2. Athalye M., Noble W. C., Minnikin D. E. ( 1985 ). Analysis of cellular fatty acids by gas chromatography as a tool in the identification of medically important coryneform bacteria. . J Appl Bacteriol 58, 507512. [View Article] [PubMed]
    [Google Scholar]
  3. Dahllöf I., Baillie H., Kjelleberg S. ( 2000 ). rpoB-based microbial community analysis avoids limitations inherent in 16S rRNA gene intraspecies heterogeneity. . Appl Environ Microbiol 66, 33763380. [View Article] [PubMed]
    [Google Scholar]
  4. Dsouza M., Taylor M. W., Ryan J., MacKenzie A., Lagutin K., Anderson R. F., Turner S. J., Aislabie J. ( 2014 ). Paenibacillus darwinianus sp. nov., isolated from gamma-irradiated Antarctic soil. . Int J Syst Evol Microbiol 64, 14061411. [View Article] [PubMed]
    [Google Scholar]
  5. Enright M. R., McInerney J. O., Griffin C. T. ( 2003 ). Characterization of endospore-forming bacteria associated with entomopathogenic nematodes, Heterorhabditis spp., and description of Paenibacillus nematophilus sp. nov.. Int J Syst Evol Microbiol 53, 435441. [View Article] [PubMed]
    [Google Scholar]
  6. Falandysz J., Dryżałowska A., Saba M., Wang J., Zhang D. ( 2014 ). Mercury in the fairy-ring of Gymnopus erythropus (Pers.) and Marasmius dryophilus (Bull.) P. Karst. mushrooms from the Gongga Mountain, Eastern Tibetan Plateau. . Ecotoxicol Environ Saf 104, 1822. [View Article] [PubMed]
    [Google Scholar]
  7. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  8. Hou B. C., Wang E. T., Li Y., Jia R. Z., Chen W. F., Man C. X., Sui X. H., Chen W. X. ( 2009 ). Rhizobial resource associated with epidemic legumes in Tibet. . Microb Ecol 57, 6981. [View Article] [PubMed]
    [Google Scholar]
  9. Judicial Commission of the International Committee for Systematics of Prokaryotes ( 2005 ). The type species of the genus Paenibacillus Ash et al. 1994 is Paenibacillus polymyxa. Opinion 77. . Int J Syst Evol Microbiol 55, 513. [View Article] [PubMed]
    [Google Scholar]
  10. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21132. Edited by Munro H. N. . New York:: Academic Press;. [View Article]
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  12. Kim J.-H., Kang H., Kim W. ( 2014 ). Paenibacillus doosanensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol 64, 12711277. [View Article] [PubMed]
    [Google Scholar]
  13. Kishore K. H., Begum Z., Pathan A. A. K., Shivaji S. ( 2010 ). Paenibacillus glacialis sp. nov., isolated from the Kafni glacier of the Himalayas, India. . Int J Syst Evol Microbiol 60, 19091913. [View Article] [PubMed]
    [Google Scholar]
  14. Li Y.-F., Calley J. N., Ebert P. J., Helmes E. B. ( 2014 ). Paenibacillus lentus sp. nov., a β-mannanolytic bacterium isolated from mixed soil samples in a selective enrichment using guar gum as the sole carbon source. . Int J Syst Evol Microbiol 64, 11661172. [View Article] [PubMed]
    [Google Scholar]
  15. Lim J.-M., Jeon C. O., Lee J.-C., Xu L.-H., Jiang C.-L., Kim C.-J. ( 2006 ). Paenibacillus gansuensis sp. nov., isolated from desert soil of Gansu Province in China. . Int J Syst Evol Microbiol 56, 21312134. [View Article] [PubMed]
    [Google Scholar]
  16. Logan N. A., Berge O., Bishop A. H., Busse H. J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L. et al. ( 2009 ). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol 59, 21142121. [View Article] [PubMed]
    [Google Scholar]
  17. Marshall B. J., Ohye D. F. ( 1966 ). Bacillus macquariensis n.sp., a psychrotrophic bacterium from sub-antarctic soil. . J Gen Microbiol 44, 4146. [View Article] [PubMed]
    [Google Scholar]
  18. Ni J. ( 2000 ). A simulation of biomes on the Tibetan Plateau and their responses to global climate change. . Mt Res Dev 20, 8089. [View Article]
    [Google Scholar]
  19. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. ( 1996 ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov.. Int J Syst Bacteriol 46, 10881092. [View Article] [PubMed]
    [Google Scholar]
  20. Reddy G. S., Prabagaran S. R., Shivaji S. ( 2008 ). Leifsonia pindariensis sp. nov., isolated from the Pindari glacier of the Indian Himalayas, and emended description of the genus Leifsonia . . Int J Syst Evol Microbiol 58, 22292234. [View Article] [PubMed]
    [Google Scholar]
  21. Saha P., Mondal A. K., Mayilraj S., Krishnamurthi S., Bhattacharya A., Chakrabarti T. ( 2005 ). Paenibacillus assamensis sp. nov., a novel bacterium isolated from a warm spring in Assam, India. . Int J Syst Evol Microbiol 55, 25772581. [View Article] [PubMed]
    [Google Scholar]
  22. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  23. Shimoyama T., Johari N. B., Tsuruya A., Nair A., Nakayama T. ( 2014 ). Paenibacillus relictisesami sp. nov., isolated from sesame oil cake. . Int J Syst Evol Microbiol 64, 15341539. [View Article] [PubMed]
    [Google Scholar]
  24. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013 ). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30, 27252729. [View Article] [PubMed]
    [Google Scholar]
  25. Yao R., Wang R., Wang D., Su J., Zheng S., Wang G. ( 2014 ). Paenibacillus selenitireducens sp. nov., a selenite-reducing bacterium isolated from a selenium mineral soil. . Int J Syst Evol Microbiol 64, 805811. [View Article] [PubMed]
    [Google Scholar]
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