1887

Abstract

A novel Gram-stain-positive, motile, catalase- and oxidase-positive, endospore-forming, facultatively anaerobic rod, designated strain JSM 100118, was isolated from an oyster () collected from the tidal flat of Naozhou Island in the South China Sea. Strain JSM 100118 was able to grow with 0–13 % (w/v) NaCl (optimum 2–5 %), at pH 5.5–10.0 (optimum pH 7.5) and at 5–50 °C (optimum 30–35 °C). The cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. The predominant respiratory quinone was menaquinone-7 and the major cellular fatty acids were anteiso-C, iso-C, C and Cω11. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unknown glycolipid and an unknown phospholipid. The genomic DNA G+C content was 35.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 100118 belonged to the genus , and was most closely related to SW-211 (98.9 % 16S rRNA gene sequence similarity), E33 (98.3 %), 4T19 (97.8 %) and D-1,5a (97.1 %). The combination of results from the phylogenetic analysis, DNA–DNA hybridization, and phenotypic and chemotaxonomic characterization supported the conclusion that strain JSM 100118 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JSM 100118 ( = CCTCC AB 2010452 = DSM 24486 = JCM 17523).

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2015-05-01
2020-01-21
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