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Volume 65, Issue Pt_5

sp. nov., an actinomycete isolated from rhizosphere soil of a peace lily ( Kochii) Free

Abstract

A novel actinomycete, designated strain NEAU-B-8, was isolated from the rhizosphere soil of a peace lily ( Kochii) collected from Heilongjiang province, north-east China. Key morphological and physiological characteristics as well as chemotaxonomic features of strain NEAU-B-8 were congruent with the description of the genus , such as the major fatty acids, the whole-cell hydrolysates, the predominant menaquinone and the phospholipid profile. The 16S rRNA gene sequence analysis revealed that strain NEAU-B-8 shared the highest sequence similarities with JCM 17982 (99.3 % 16S rRNA gene sequence similarity), TT07I-57 (98.4 %), IY07-55 (98.3 %) and TT04-21 (98.2 %); similarities to type strains of other species of this genus were lower than 98 %. The phylogenetic tree based on 16S rRNA gene sequences showed that strain NEAU-B-8 formed a distinct branch with JCM 17982 that was supported by a high bootstrap value of 97 % in the neighbour-joining tree and was also recovered with the maximum-likelihood algorithm. However, the DNA–DNA relatedness between strain NEAU-B-8 and JCM 17982 was found to be 50.6±1.2 %. Meanwhile, strain NEAU-B-8 differs from other most closely related strains in phenotypic properties, such as maximum NaCl tolerance, hydrolysis of aesculin and decomposition of urea. On the basis of the morphological, physiological, chemotaxonomic, phylogenetic and DNA–DNA hybridization data, we conclude that strain NEAU-B-8 represents a novel species of the genus , named sp. nov. The type strain is NEAU-B-8. ( = CGMCC 4.7134 = DSM 46673).

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Funding
This study was supported by the:
  • National Outstanding Youth Foundation (Award 31225024)
  • National Key Project for Basic Research (Award 2010CB126102)
  • National Key Technology R&D Program (Award 2012BAD19B06)
  • Program for New Century Excellent Talents in University (Award NCET-11-0953)
  • National Natural Science Foundation of China (Award 31471832, 31071750, 31171913 and 31372006)
  • Outstanding Youth Foundation of Heilongjiang Province (Award JC201201)
  • hang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP)
  • Science and Technology Research Project of Heilongjiang Provincial Educational Commission (Award 12541001)
  • Youth Science Foundation of Heilongjiang Province (Award QC2014C013)
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2015-05-01
2024-05-02
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References

  1. Collins M. D. ( 1985 ). Isoprenoid quinone analyses in bacterial classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–284. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
     [Google Scholar]
  2. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133–142. [View Article] [PubMed]
    [Google Scholar]
  3. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368–376. [View Article] [PubMed]
    [Google Scholar]
  4. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783–789. [View Article]
    [Google Scholar]
  5. Gao R. X., Liu C. X., Zhao J. W., Jia F. Y., Yu C., Yang L. Y., Wang X. J., Xiang W. S. ( 2014 ). Micromonospora jinlongensis sp. nov., isolated from muddy soil in China and emended description of the genus Micromonospora . . Antonie van Leeuwenhoek 105, 307–315. [View Article] [PubMed]
    [Google Scholar]
  6. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. ( 1974 ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24, 54–63. [View Article]
    [Google Scholar]
  7. Hayakawa M., Nonomura H. ( 1987 ). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. . J Ferment Technol 65, 501–509. [View Article]
    [Google Scholar]
  8. Huss V. A. R., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184–192. [View Article] [PubMed]
    [Google Scholar]
  9. Jiang Y., Wiese J., Tang S. K., Xu L. H., Imhoff J. F., Jiang C. L. ( 2008 ). Actinomycetospora chiangmaiensis gen. nov., sp. nov., a new member of the family Pseudonocardiaceae . . Int J Syst Evol Microbiol 58, 408–413. [View Article] [PubMed]
    [Google Scholar]
  10. Kelly K. L. ( 1964 ). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC:: US Government Printing Office;.
     [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  12. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111–120. [View Article] [PubMed]
    [Google Scholar]
  13. Lechevalier M. P., Lechevalier H. A. ( 1980 ). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy (Special Publication no. 6), pp. 277–291. Edited by Dietz A., Thayer J. . Arlington, VA:: Society for Industrial Microbiology;.
     [Google Scholar]
  14. Lee Y. K., Kim H. W., Liu C. L., Lee H. K. ( 2003 ). A simple method for DNA extraction from marine bacteria that produce extracellular materials. . J Microbiol Methods 52, 245–250. [View Article] [PubMed]
    [Google Scholar]
  15. Loqman S., Barka E. A., Clément C., Ouhdouch Y. ( 2009 ). Antagonistic actinomycetes from Moroccan soil to control the grapevine gray mold. . World J Microbiol Biotechnol 25, 81–91. [View Article]
    [Google Scholar]
  16. Mandel M., Marmur J. ( 1968 ). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12B, 195–206. [View Article]
    [Google Scholar]
  17. McKerrow J., Vagg S., McKinney T., Seviour E. M., Maszenan A. M., Brooks P., Seviour R. J. ( 2000 ). A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. . Lett Appl Microbiol 30, 178–182. [View Article] [PubMed]
    [Google Scholar]
  18. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  19. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  20. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16, 313–340. [View Article]
    [Google Scholar]
  21. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  22. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 a). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  23. Tamura T., Ishida Y., Hamada M., Otoguro M., Yamamura H., Hayakawa M., Suzuki K. ( 2011 b). Description of Actinomycetospora chibensis sp. nov., Actinomycetospora chlora sp. nov., Actinomycetospora cinnamomea sp. nov., Actinomycetospora corticicola sp. nov., Actinomycetospora lutea sp. nov., Actinomycetospora straminea sp. nov. and Actinomycetospora succinea sp. nov. and emended description of the genus Actinomycetospora . . Int J Syst Evol Microbiol 61, 1275–1280. [View Article] [PubMed]
    [Google Scholar]
  24. Waksman S. A. ( 1961 ). The Actinomycetes, vol. 2, Classification, Identification and Descriptions of Genera and Species. Baltimore:: Williams & Wilkins;.
     [Google Scholar]
  25. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
  26. Wu C., Lu X., Qin M., Wang Y., Ruan J. ( 1989 ). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16, 176–178. [View Article]
    [Google Scholar]
  27. Xiang W. S., Liu C. X., Wang X. J., Du J., Xi L. J., Huang Y. ( 2011 ). Actinoalloteichus nanshanensis sp. nov., isolated from the rhizosphere of a fig tree (Ficus religiosa). . Int J Syst Evol Microbiol 61, 1165–1169. [View Article] [PubMed]
    [Google Scholar]
  28. Xie Q. Y., Lin H. P., Li L., Brown R., Goodfellow M., Deng Z. X., Hong K. ( 2012 ). Verrucosispora wenchangensis sp. nov., isolated from mangrove soil. . Antonie van Leeuwenhoek 102, 1–7. [View Article] [PubMed]
    [Google Scholar]
  29. Yokota A., Tamura T., Hasegawa T., Huang L. H. ( 1993 ). Catenuloplanes japonicus gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales . . Int J Syst Bacteriol 43, 805–812. [View Article]
    [Google Scholar]
  30. Zhang Y. J., Liu C. X., Zhang J., Shen Y., Li C., He H. R., Wang X. J., Xiang W. S. ( 2014 ). Actinomycetospora atypica sp. nov., a novel soil actinomycete and emended description of the genus Actinomycetospora . . Antonie van Leeuwenhoek 105, 891–897. [View Article] [PubMed]
    [Google Scholar]
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Actinomycetospora rhizophila sp. nov., an actinomycete isolated from rhizosphere soil of a peace lily (Spathi phyllum Kochii)
Int J Syst Evol Microbiol 65, 1520 (2015); https://doi.org/10.1099/ijs.0.000131
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