1887

Abstract

An alkaliphilic and moderately halophilic actinobacterium, designated strain GP10-3, was isolated from collected from the southern edge of Taklimakan desert, Xinjiang, China. Cells of this strain were Gram-stain-positive, non-motile and non-spore-forming short rods. Strain GP10-3 grew optimally at 37 °C on LB agar media in the presence of 5–10 % (w/v) NaCl at pH 9.0. The menaquinones were MK-7, MK-8 and MK-9. The major fatty acids (>10 %) were anteiso-C, anteiso-C and iso-C. The peptidoglycan type was variation A4α, -Lys--Glu. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylglycolipid, phosphatidylcholine, phosphatidylinositol, glycolipid and an unidentified phospholipid. The DNA G+C content was 67.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain GP10-3 belonged to the genus , sharing 94.6–96.9 % sequence similarity with the type strains of species within this genus with validly published names. Based on the evidence of the polyphasic taxonomic study, strain GP10-3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GP10-3 ( = DSM 27959 = KCTC 29119).

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2015-05-01
2019-12-05
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References

  1. Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef] [PubMed]
    [Google Scholar]
  2. Collins M. D. , Lawson P. A. , Labrenz M. , Tindall B. J. , Weiss N. , Hirsch P. . ( 2002; ). Nesterenkonia lacusekhoensis sp. nov., isolated from hypersaline Ekho Lake, East Antarctica, and emended description of the genus Nesterenkonia . . Int J Syst Evol Microbiol 52:, 1145–1150. [CrossRef] [PubMed]
    [Google Scholar]
  3. Cowan S. T. , Steel K. J. . ( 1965; ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  4. Delgado O. , Quillaguamán J. , Bakhtiar S. , Mattiasson B. , Gessesse A. , Hatti-Kaul R. . ( 2006; ). Nesterenkonia aethiopica sp. nov., an alkaliphilic, moderate halophile isolated from an Ethiopian soda lake. . Int J Syst Evol Microbiol 56:, 1229–1232. [CrossRef] [PubMed]
    [Google Scholar]
  5. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  6. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  7. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  8. Govender L. , Naidoo L. , Setati M. E. . ( 2013; ). Nesterenkonia suensis sp. nov., a haloalkaliphilic actinobacterium isolated from a salt pan. . Int J Syst Evol Microbiol 63:, 41–46. [CrossRef] [PubMed]
    [Google Scholar]
  9. Groth I. , Schumann P. , Rainey F. A. , Martin K. , Schuetze B. , Augsten K. . ( 1997; ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47:, 1129–1133. [CrossRef] [PubMed]
    [Google Scholar]
  10. Guo L. , Tuo L. , Habden X. , Zhang Y. , Liu J. , Jiang Z. , Liu S. , Dilbar T. , Sun C. . ( 2015; ). Allosalinactinospora lopnorensis gen. nov., sp. nov., a new member of the family Nocardiopsaceae isolated from soil. . Int J Syst Evol Microbiol 65:, 206–213.[PubMed] [CrossRef]
    [Google Scholar]
  11. Hall T. A. . ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  12. Jones K. L. . ( 1949; ). Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. . J Bacteriol 57:, 141–145.[PubMed]
    [Google Scholar]
  13. Kämpfer P. , Kroppenstedt R. M. , Dott W. . ( 1991; ). A numerical classification of the genera Streptomyces and Streptoverticillium using miniaturized physiological tests. . J Gen Microbiol 137:, 1831–1891. [CrossRef]
    [Google Scholar]
  14. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. et al. ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kimura M. . ( 1983; ). The Neutral Theory of Molecular Evolution. London:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  16. Kuykendall L. D. , Roy M. A. , O'Neill J. J. , Devine T. E. . ( 1988; ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradorhizobium japonicum . . Int J Syst Bacteriol 38:, 358–361. [CrossRef]
    [Google Scholar]
  17. Lányí B. . ( 1987; ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  18. Li W.-J. , Chen H.-H. , Zhang Y.-Q. , Schumann P. , Stackebrandt E. , Xu L.-H. , Jiang C.-L. . ( 2004; ). Nesterenkonia halotolerans sp. nov. and Nesterenkonia xinjiangensis sp. nov., actinobacteria from saline soils in the west of China. . Int J Syst Evol Microbiol 54:, 837–841. [CrossRef] [PubMed]
    [Google Scholar]
  19. Li W.-J. , Chen H.-H. , Kim C.-J. , Zhang Y.-Q. , Park D.-J. , Lee J.-C. , Xu L.-H. , Jiang C.-L. . ( 2005; ). Nesterenkonia sandarakina sp. nov. and Nesterenkonia lutea sp. nov., novel actinobacteria, and emended description of the genus Nesterenkonia . . Int J Syst Evol Microbiol 55:, 463–466. [CrossRef] [PubMed]
    [Google Scholar]
  20. Li W.-J. , Xu P. , Schumann P. , Zhang Y.-Q. , Pukall R. , Xu L.-H. , Stackebrandt E. , Jiang C.-L. . ( 2007; ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . . Int J Syst Evol Microbiol 57:, 1424–1428. [CrossRef] [PubMed]
    [Google Scholar]
  21. Li W.-J. , Zhang Y.-Q. , Schumann P. , Liu H.-Y. , Yu L.-Y. , Zhang Y.-Q. , Stackebrandt E. , Xu L.-H. , Jiang C.-L. . ( 2008; ). Nesterenkonia halophila sp. nov., a moderately halophilic, alkalitolerant actinobacterium isolated from a saline soil. . Int J Syst Evol Microbiol 58:, 1359–1363. [CrossRef] [PubMed]
    [Google Scholar]
  22. Luo H.-Y. , Miao L.-H. , Fang C. , Yang P.-L. , Wang Y.-R. , Shi P.-J. , Yao B. , Fan Y.-L. . ( 2008; ). Nesterenkonia flava sp. nov., isolated from paper-mill effluent. . Int J Syst Evol Microbiol 58:, 1927–1930. [CrossRef] [PubMed]
    [Google Scholar]
  23. Luo H.-Y. , Wang Y.-R. , Miao L.-H. , Yang P.-L. , Shi P.-J. , Fang C.-X. , Yao B. , Fan Y.-L. . ( 2009; ). Nesterenkonia alba sp. nov., an alkaliphilic actinobacterium isolated from the black liquor treatment system of a cotton pulp mill. . Int J Syst Evol Microbiol 59:, 863–868. [CrossRef] [PubMed]
    [Google Scholar]
  24. Magee C. M. , Rodeheaver G. , Edgerton M. T. , Edlich R. F. . ( 1975; ). A more reliable gram staining technic for diagnosis of surgical infections. . Am J Surg 130:, 341–346. [CrossRef] [PubMed]
    [Google Scholar]
  25. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  26. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  27. Miller J. H. . ( 1972; ). Experiments in Molecular Genetics. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  28. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  29. Mota R. R. , Márquez M. C. , Arahal D. R. , Mellado E. , Ventosa A. . ( 1997; ). Polyphasic taxonomy of Nesterenkonia halobia . . Int J Syst Bacteriol 47:, 1231–1235. [CrossRef] [PubMed]
    [Google Scholar]
  30. Onishi H. , Kamekura M. . ( 1972; ). Micrococcus halobius sp. n.. Int J Syst Bacteriol 22:, 233–236. [CrossRef]
    [Google Scholar]
  31. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  32. Schleifer K. H. , Kandler O. . ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  33. Schumann P. . ( 2011; ). Peptidoglycan structure. . Methods Microbiol 38:, 101–129. [CrossRef]
    [Google Scholar]
  34. Shirling E. B. , Gottlieb D. . ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  35. Stackebrandt E. . ( 2012; ). Genus VI. Nesterenkonia Stackebrandt, Koch, Gvozdiak & Schumann 1995, 689VP . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol 5, pp. 636–642. Edited by Goodfellow M , Kämpfer P , Busse H.-J. , Trujillo M. E. , Suzuki K. , Ludwig W. , Whitman W. B. . . New York:: Springer;.
    [Google Scholar]
  36. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  37. Stackebrandt E. , Koch C. , Gvozdiak O. , Schumann P. . ( 1995; ). Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend.. Int J Syst Bacteriol 45:, 682–692. [CrossRef] [PubMed]
    [Google Scholar]
  38. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  39. Waksman S. A. . ( 1961; ). The Actinomycetes. Classification, Identification and Description of Genera and Species, vol. II. Baltimore, MD:: Williams & Wilkins;.
    [Google Scholar]
  40. Wang J. , Li Y. , Bian J. , Tang S.-K. , Ren B. , Chen M. , Li W.-J. , Zhang L.-X. . ( 2010; ). Prauserella marina sp. nov., isolated from ocean sediment of the South China Sea. . Int J Syst Evol Microbiol 60:, 985–989. [CrossRef] [PubMed]
    [Google Scholar]
  41. Wang H.-F. , Zhang Y.-G. , Chen J.-Y. , Hozzein W. N. , Li L. , Wadaan M. A. M. , Zhang Y.-M. , Li W.-J. . ( 2014; ). Nesterenkonia rhizosphaerae sp. nov., an alkaliphilic actinobacterium isolated from rhizosphere soil in saline-alkaline desert. . Int J Syst Evol Microbiol 64:, 4021–4026. [CrossRef] [PubMed]
    [Google Scholar]
  42. Williams S. T. , Goodfellow M. , Alderson G. , Wellington E. M. H. , Sneath P. H. A. , Sackin M. J. . ( 1983; ). Numerical classification of Streptomyces and related genera. . J Gen Microbiol 129:, 1743–1813.[PubMed]
    [Google Scholar]
  43. Yoon J.-H. , Jung S.-Y. , Kim W. , Nam S.-W. , Oh T.-K. . ( 2006; ). Nesterenkonia jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. . Int J Syst Evol Microbiol 56:, 2587–2592. [CrossRef] [PubMed]
    [Google Scholar]
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