1887

Abstract

Two actinobacterial strains, m20 and z8, were isolated from soil taken from rainforest areas/tropic forest region, Yunnan Province, south-west China. The 16S rRNA gene sequence similarities and DNA–DNA relatedness values between strains m20 and z8 were 100 and 88.2 %, respectively, which indicated that these two strains should be classified as the same species. The taxonomic position of the strains was determined by a polyphasic approach. Morphological and chemotaxonomic features of the strains were consistent with those of the genus . A phylogenetic tree based on 16S rRNA gene sequences showed that strains m20 and z8 formed an evolutionary branch within the genus and shared relatively high 16S rRNA gene sequence similarity values with other members of this genus, including ‘’ NBRC 108799 (98.95 %), NBRC 108882 (98.25 %), NBRC 16668 (98.11 %), ATCC 29050 (98.11 %) and ATCC 15420 (98.11 %). DNA–DNA relatedness values between strain m20 and the five above-mentioned strains were 56.3, 55.1, 52.8 , 50.1 and 48.4 %, respectively. On the basis of phenotypic, genotypic and phylogenetic properties, strains m20 and z8 could be distinguished from phylogenetically related members of the genus . The isolates thus merit species status within the genus , for which the name http://dx.doi.org/10.1601/nm.6817 sp. nov. is proposed. The type strain is m20 ( = CGMCC 4.7111 = KCTC 29196). Strain z8 ( = CGMCC 4.7112 = KCTC 29197) is a reference strain.

Funding
This study was supported by the:
  • Beijing Natural Science Foundation (Award 5152017)
  • National Natural Science Foundation of China
  • NSFC (Award 31070002)
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2015-05-01
2022-01-26
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