1887

Abstract

Two actinobacterial strains, m20 and z8, were isolated from soil taken from rainforest areas/tropic forest region, Yunnan Province, south-west China. The 16S rRNA gene sequence similarities and DNA–DNA relatedness values between strains m20 and z8 were 100 and 88.2 %, respectively, which indicated that these two strains should be classified as the same species. The taxonomic position of the strains was determined by a polyphasic approach. Morphological and chemotaxonomic features of the strains were consistent with those of the genus . A phylogenetic tree based on 16S rRNA gene sequences showed that strains m20 and z8 formed an evolutionary branch within the genus and shared relatively high 16S rRNA gene sequence similarity values with other members of this genus, including ‘’ NBRC 108799 (98.95 %), NBRC 108882 (98.25 %), NBRC 16668 (98.11 %), ATCC 29050 (98.11 %) and subsp ATCC 15420 (98.11 %). DNA–DNA relatedness values between strain m20 and the five above-mentioned strains were 56.3, 55.1, 52.8 , 50.1 and 48.4 %, respectively. On the basis of phenotypic, genotypic and phylogenetic properties, strains m20 and z8 could be distinguished from phylogenetically related members of the genus . The isolates thus merit species status within the genus , for which the name http://dx.doi.org/10.1601/nm.6817 sp. nov. is proposed. The type strain is m20 ( = CGMCC 4.7111 = KCTC 29196). Strain z8 ( = CGMCC 4.7112 = KCTC 29197) is a reference strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000121
2015-05-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/5/1467.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000121&mimeType=html&fmt=ahah

References

  1. Bérdy J.. ( 2005; ). Bioactive microbial metabolites. . J Antibiot (Tokyo) 58:, 1–26. [CrossRef] [PubMed]
    [Google Scholar]
  2. Collins M. D., Howarth O. W., Grund E., Kroppenstedt R. M.. ( 1987; ). Isolation and structural determination of new members of the vitamin K2 series in Nocardia brasiliensis. . FEMS Microbiol Lett 41:, 35–39. [CrossRef]
    [Google Scholar]
  3. Cui X.-L., Mao P.-H., Zeng M., Li W.-J., Zhang L.-P., Xu L.-H., Jiang C.-L.. ( 2001; ). Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. . Int J Syst Evol Microbiol 51:, 357–363.[PubMed]
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A.. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  5. Dong X.-Z., Cai M.-Y.. (editors) ( 2001; ). Determination of biochemical properties. . In Manual for the Systematic Identification of General Bacteria, pp. 370–398. Beijing:: Science Press; (in Chinese).
    [Google Scholar]
  6. Felsenstein J.. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  7. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N.. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  8. Hasegawa T., Takizawa M., Tanida S.. ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Appl Microbiol 29:, 319–322. [CrossRef]
    [Google Scholar]
  9. Huß V. A., Festl H., Schleifer K. H.. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef] [PubMed]
    [Google Scholar]
  10. Jahnke K.-D.. ( 1992; ). basic computer program for evaluation of spectroscopic DNA renaturation data from Gilford System 2600 spectrophotometer on a PC/XT/AT type personal computer. . J Microbiol Methods 15:, 61–73. [CrossRef]
    [Google Scholar]
  11. Jones K. L.. ( 1949; ). Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. . J Bacteriol 57:, 141–145.[PubMed]
    [Google Scholar]
  12. Kämpfer P., Kroppenstedt R. M.. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42:, 989–1005. [CrossRef]
    [Google Scholar]
  13. Kelly K. L.. ( 1964; ). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kimura M.. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  16. Kluge A. G., Farris J. S.. ( 1969; ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18:, 1–32. [CrossRef]
    [Google Scholar]
  17. Lazzarini A., Cavaletti L., Toppo G., Marinelli F.. ( 2000; ). Rare genera of actinomycetes as potential producers of new antibiotics. . Antonie van Leeuwenhoek 78:, 399–405. [CrossRef] [PubMed]
    [Google Scholar]
  18. Lechevalier M. P., Lechevalier H. A.. ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20:, 435–443. [CrossRef]
    [Google Scholar]
  19. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L.. ( 2007; ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. . Int J Syst Evol Microbiol 57:, 1424–1428. [CrossRef] [PubMed]
    [Google Scholar]
  20. Lu Z., Liu Z., Wang L., Zhang Y., Qi W., Goodfellow M.. ( 2001; ). Saccharopolyspora flava sp. nov. and Saccharopolyspora thermophila sp.nov., novel actinomycetes from soil. . Int J Syst Evol Microbiol 51:, 319–325.[PubMed]
    [Google Scholar]
  21. Marmur J., Doty P.. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef] [PubMed]
    [Google Scholar]
  22. McCarthy A. J., Williams S. T.. ( 1992; ). Actinomycetes as agents of biodegradation in the environment–a review. . Gene 115:, 189–192. [CrossRef] [PubMed]
    [Google Scholar]
  23. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H.. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  24. Saitou N., Nei M.. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  25. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  26. Shirling E. B., Gottlieb D.. ( 1966; ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  27. Shirling E. B., Gottlieb D.. ( 1968; a). Cooperative description of type cultures of Streptomyces. II. Species descriptions from first study. . Int J Syst Bacteriol 18:, 69–189. [CrossRef]
    [Google Scholar]
  28. Shirling E. B., Gottlieb D.. ( 1968; b). Cooperative description of type cultures of Streptomyces. III. Additional species descriptions from first and second studies. . Int J Syst Bacteriol 18:, 279–392. [CrossRef]
    [Google Scholar]
  29. Shirling E. B., Gottlieb D.. ( 1969; ). Cooperative description of type cultures of Streptomyces. IV. Species descriptions from the second, third and fourth studies. . Int J Syst Bacteriol 19:, 391–512. [CrossRef]
    [Google Scholar]
  30. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  31. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  32. Waksman S. A., Henrici A. T.. ( 1943; ). The nomenclature and classification of the actinomycetes. . J Bacteriol 46:, 337–341.[PubMed]
    [Google Scholar]
  33. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  34. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J.. ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173:, 697–703.[PubMed]
    [Google Scholar]
  35. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J.. ( 1983; ). Numerical classification of Streptomyces and related genera. . J Gen Microbiol 129:, 1743–1813.[PubMed]
    [Google Scholar]
  36. Williams S. T., Goodfellow M., Alderson G.. ( 1989; ). Genus Streptomyces Waksman and Henrici 1943, 339AL. . In Bergey’s Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by Williams S. T., Sharpe M. E., Holt J. G... Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  37. Wu C., Lu X., Qin M., Wang Y., Ruan J.. ( 1989; ). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16:, 176–178 [CrossRef]
    [Google Scholar]
  38. Zhao X. Q., Li W. J., Jiao W. C., Li Y., Yuan W. J., Zhang Y. Q., Klenk H. P., Suh J. W., Bai F. W.. ( 2009; ). Streptomyces xinghaiensis sp. nov., isolated from marine sediment. . Int J Syst Evol Microbiol 59:, 2870–2874. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000121
Loading
/content/journal/ijsem/10.1099/ijs.0.000121
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error