1887

Abstract

The Gram-stain-negative, rod-shaped bacterial strain designated RU-1-R-18 was isolated from intertidal sediment on Sakhalin Island in Russia. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RU-1-R-18 was related to the genus and shared highest sequence similarities with the type strain KACC 16875 (97.9 %). The predominant cellular fatty acid was Cω7. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one unidentified aminophospholipid and seven unidentified polar lipids. The genomic DNA G+C content of strain RU-1-R-18 was 59.1 mol%. Combined data from phenotypic, phylogenetic and DNA–DNA relatedness studies demonstrated that strain RU-1-R-18 represents a novel species of the genus , for which the name sp. nov. is proposed (type strain RU-1-R-18 = LMG 28384 = DSM 29052).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000119
2015-05-01
2020-01-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/5/1462.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000119&mimeType=html&fmt=ahah

References

  1. Alarico S., Rainey F. A., Empadinhas N., Schumann P., Nobre M. F., da Costa M. S.. ( 2002; ). Rubritepida flocculans gen. nov., sp. nov., a new slightly thermophilic member of the α-1 subclass of the Proteobacteria. . Syst Appl Microbiol 25:, 198–206. [CrossRef] [PubMed]
    [Google Scholar]
  2. Boldareva E. N., Turova T. P., Kolganova T. V., Moskalenko A. A., Makhneva Z. K., Gorlenko V. M.. ( 2009; ). [Roseococcus suduntuyensis sp. nov., a new aerobic bacteriochlorophyll A-containing bacterium isolated from a low-mineralized soda lake of Eastern Siberia]. . Mikrobiologiia 78:, 106–116 (in Russian).[PubMed]
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A.. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  4. Huß V. A. R., Festl H., Schleifer K. H.. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192. [CrossRef] [PubMed]
    [Google Scholar]
  5. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  6. Liu C., Zhang X.-Y., Su H.-N., Zhou M.-Y., Chen B., Li H., Chen X.-L., Zhao D.-L., Zhou B.-C. et al. ( 2014; ). Puniceibacterium antarcticum gen. nov., sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 64:, 1566–1572. [CrossRef] [PubMed]
    [Google Scholar]
  7. Margesin R., Zhang D.-C.. ( 2013; ). Humitalea rosea gen. nov., sp. nov., an aerobic bacteriochlorophyll-containing bacterium of the family Acetobacteraceae isolated from soil. . Int J Syst Evol Microbiol 63:, 1411–1416. [CrossRef] [PubMed]
    [Google Scholar]
  8. Margesin R., Gander S., Zacke G., Gounot A. M., Schinner F.. ( 2003; ). Hydrocarbon degradation and enzyme activities of cold-adapted bacteria and yeasts. . Extremophiles 7:, 451–458. [CrossRef] [PubMed]
    [Google Scholar]
  9. Marmur J., Doty P.. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef] [PubMed]
    [Google Scholar]
  10. Reasoner, D. J. & Geldreich, E. E. (1985). A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49, 1–7.
  11. Saitou N., Nei M.. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  12. Sambrook J., Frisch E. F., Maniatis T.. ( 1989; ). Molecular Cloning: a Laboratory Manual, , 2nd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  13. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  14. Süßmuth R., Eberspächer J., Haag R., Springer W.. ( 1987; ). Biochemisch-mikrobiologisches Praktikum. Stuttgart:: Georg Thieme;.
    [Google Scholar]
  15. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  16. Tindall B. J.. ( 1990; a). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  17. Tindall B. J.. ( 1990; b). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  18. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  19. Zhang D.-C., Margesin R.. ( 2014; ). Martelella radicis sp. nov. and Martelella mangrovi sp. nov., isolated from mangrove sediment. . Int J Syst Evol Microbiol 64:, 3104–3108. [CrossRef] [PubMed]
    [Google Scholar]
  20. Zhang D.-C., Wang H.-X., Liu H.-C., Dong X.-Z., Zhou P.-J.. ( 2006; ). Flavobacterium glaciei sp. nov., a novel psychrophilic bacterium isolated from the China No.1 glacier. . Int J Syst Evol Microbiol 56:, 2921–2925. [CrossRef] [PubMed]
    [Google Scholar]
  21. Zhang D.-C., Schinner F., Margesin R.. ( 2010; ). Pedobacter bauzanensis sp. nov., isolated from soil. . Int J Syst Evol Microbiol 60:, 2592–2595. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000119
Loading
/content/journal/ijsem/10.1099/ijs.0.000119
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error