Multiple isolates of the genus were obtained from the haemolymph of wild-caught fruit flies. Sixteen isolates were distinguished from the six previously described species based on 16S rRNA gene sequences. These isolates belonged to two distinct groups, which we propose each comprise previously undescribed species. Two isolates, designated A and B, were characterized by DNA sequences of the , , , and housekeeping genes, whole-genome DNA–DNA hybridizations with their nearest relatives and utilization of substrates for metabolism. The closest phylogenetic relatives of strain A are strain B (86.9 % identity for the housekeeping genes) and DSM 4539 (86.0 % identity). The closest phylogenetic relatives of strain B are strain A (86.9 % identity) and DSM 4539 (86.6 % identity). The type strains of described species in this genus shared between 84.1 and 90.1 % identity for these sequences. DNA–DNA hybridization between the strain pairs A–B, A DSM 4539 and B DSM 4539 all resulted in less than 25 % relatedness. In addition, patterns of utilization of amygdalin, arbutin, aesculin, salicin, -sorbitol, trehalose, inositol, -adonitol and -galactose distinguish strains A and B from other members of this genus. Strains A and B therefore represent novel species, for which the names sp. nov. (type strain A =DSM 19967 =ATCC BAA-1589) and sp. nov. (type strain B =DSM 19968 =ATCC BAA-1590) are proposed.


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vol. , part 5, pp. 1108 - 1111

Neighbour-joining phylogenetic tree based on partial 16S rRNA gene sequences (978 nt), showing the positions of the novel strains within the genus

PCR primer sequences for specific amplification of housekeeping genes from species

GenBank/EMBL/DDBJ accession numbers of sequences determined in this study

[PDF file of Supplementary Fig. S1 and Supplementary Tables S1 and S2](59 KB)


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