1887

Abstract

Multiple isolates of the genus were obtained from the haemolymph of wild-caught fruit flies. Sixteen isolates were distinguished from the six previously described species based on 16S rRNA gene sequences. These isolates belonged to two distinct groups, which we propose each comprise previously undescribed species. Two isolates, designated A and B, were characterized by DNA sequences of the , , , and housekeeping genes, whole-genome DNA–DNA hybridizations with their nearest relatives and utilization of substrates for metabolism. The closest phylogenetic relatives of strain A are strain B (86.9 % identity for the housekeeping genes) and DSM 4539 (86.0 % identity). The closest phylogenetic relatives of strain B are strain A (86.9 % identity) and DSM 4539 (86.6 % identity). The type strains of described species in this genus shared between 84.1 and 90.1 % identity for these sequences. DNA–DNA hybridization between the strain pairs A–B, A DSM 4539 and B DSM 4539 all resulted in less than 25 % relatedness. In addition, patterns of utilization of amygdalin, arbutin, aesculin, salicin, -sorbitol, trehalose, inositol, -adonitol and -galactose distinguish strains A and B from other members of this genus. Strains A and B therefore represent novel species, for which the names sp. nov. (type strain A =DSM 19967 =ATCC BAA-1589) and sp. nov. (type strain B =DSM 19968 =ATCC BAA-1590) are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000117-0
2009-05-01
2019-12-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/5/1108.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000117-0&mimeType=html&fmt=ahah

References

  1. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  2. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  3. Farmer, J. J., III, Davis, B. R., Hickman-Brenner, F. W., McWhorter, A., Huntley-Carter, G. P., Asbury, M. A., Riddle, C., Wathen-Grady, H. G., Elias, C. & other authors ( 1985; ). Biochemical identification of new species and biogroups of Enterobacteriaceae isolated from clinical specimens. J Clin Microbiol 21, 46–76.
    [Google Scholar]
  4. Hickman-Brenner, F. W., Farmer, J. J., III, Steigerwalt, A. G. & Brenner, D. J. ( 1983; ). Providencia rustigianii: a new species in the family Enterobacteriaceae formerly known as Providencia alcalifaciens biogroup 3. J Clin Microbiol 17, 1057–1060.
    [Google Scholar]
  5. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  6. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  7. Müller, H. E., O'Hara, C. M., Fanning, G. R., Hickman-Brenner, F. W., Swenson, J. M. & Brenner, D. J. ( 1986; ). Providencia heimbachae, a new species of Enterobacteriaceae isolated from animals. Int J Syst Bacteriol 36, 252–256.[CrossRef]
    [Google Scholar]
  8. Penner, J. L. & Hennessy, J. N. ( 1979; ). Application of O-serotyping in a study of Providencia rettgeri (Proteus rettgeri) isolated from human and nonhuman sources. J Clin Microbiol 10, 834–840.
    [Google Scholar]
  9. Santos, S. R. & Ochman, H. ( 2004; ). Identification and phylogenetic sorting of bacterial lineages with universally conserved genes and proteins. Environ Microbiol 6, 754–759.[CrossRef]
    [Google Scholar]
  10. Somvanshi, V. S., Lang, E., Sträubler, B., Spröer, C., Schumann, P., Ganguly, S., Saxena, A. K. & Stackebrandt, E. ( 2006; ). Providencia vermicola sp. nov., isolated from infective juveniles of the entomopathogenic nematode Steinernema thermophilum. Int J Syst Evol Microbiol 56, 629–633.[CrossRef]
    [Google Scholar]
  11. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  12. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J. ( 1991; ). 16S ribosomal DNA amplification in phylogenetic study. J Bacteriol 173, 697–703.
    [Google Scholar]
  13. Yoh, M., Matsuyama, J., Ohnishi, M., Takagi, K., Miyagi, H., Mori, K., Park, K., Ono, T. & Honda, T. ( 2005; ). Importance of Providencia species as a major cause of travellers' diarrhoea. J Med Microbiol 54, 1077–1082.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000117-0
Loading
/content/journal/ijsem/10.1099/ijs.0.000117-0
Loading

Data & Media loading...

Supplements

vol. , part 5, pp. 1108 - 1111

Neighbour-joining phylogenetic tree based on partial 16S rRNA gene sequences (978 nt), showing the positions of the novel strains within the genus

PCR primer sequences for specific amplification of housekeeping genes from species

GenBank/EMBL/DDBJ accession numbers of sequences determined in this study

[PDF file of Supplementary Fig. S1 and Supplementary Tables S1 and S2](59 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error