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Volume 65, Issue Pt_5

sp. nov., isolated from bark Free

Abstract

Five non-spore-forming, aerobic and Gram-stain-positive bacterial strains, 10-107-8, 1C-4, NHI3_6, 4107_1_2, and 3D-3, were isolated from × bark collected in Puyang City, Henan Province, PR China. The isolates grew at 15–40 °C and pH 5–10. The optimum temperature and pH for growth were 30 °C and pH 8.0, respectively. Chemotaxonomic features included MK-10 and MK-11 as major menaquinones (type strain); predominating iso- and anteiso-branched cellular fatty acids; diphosphatidylglycerol and phosphatidylglycerol as major polar lipids (type strain); ornithine as the principal diamino acid of the cell-wall peptidoglycan (type strain); glycolyl type as cell-wall acyl type; and DNA G+C content of 66.8–67.6 mol%. These features were consistent with classification in the genus . Analysis of 16S rRNA gene sequence data indicated that the five isolates belonged to the genus and were closely related to . A high 16S rRNA gene sequence similarity of 96.97 % to YIM 70130 was observed. The five isolates showed less than 96.20 % 16S rRNA gene sequence similarity to the other species of the genus with validly published names. DNA–DNA relatedness of the five isolates with JCM 13013 ranged from 35.62 % to 44.36 %. Considering the results of 16S rRNA gene sequence analysis and the physiological and biochemical characteristics, we propose that the five strains should be assigned to a novel species of the genus . The name proposed for the five strains is sp. nov., and the type strain is 10-107-8 ( = CFCC 11275 = KCTC 29152).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31200486)
  • National Infrastructure of Microbial Resources (Award NIMR-2014-7)
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2015-05-01
2024-05-03
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Microbacterium populi sp. nov., isolated from Populus×euramericana bark
Int J Syst Evol Microbiol 65, 1444 (2015); https://doi.org/10.1099/ijs.0.000117
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