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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_5

sp. nov. isolated from marine sediment Free

Abstract

A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium designated SGD-V-25 was isolated from Veraval sediment sample, India. Strain SGD-V-25 was capable of growing at 25–50 °C (optimum 37 °C), pH 6–12 (optimum pH 7.0) and with 0–5 % (w/v) NaCl. The taxonomic position of this strain was deduced using a polyphasic approach and the 16S rRNA gene sequence analysis showed that the isolate belongs to the phylum , forming the cluster with MTCC 1548, with which it shares highest similarity of 99.1 % with 13 nt differences. Other type strains of the genus showed less than 96 % similarity. The cell wall contained -diaminopimelic acid as the diagnostic diamino acid. The polar lipid profile of strain SGD-V-25 showed the presence of diphosphatidylglycerol, phosphatidylglycerol, phsophoglycolipid and two aminophospholipids. The predominant isoprenoid quinone was MK-7. The major cellular fatty acids were iso-C, anteiso-C, anteiso-C, iso-C, Cω11 and C The genomic DNA G+C content of strain SGD-V-25 was 37.6 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA hybridization, strain SGD-V-25 could be clearly distinguished from closely related members of the genus , and the name sp. nov., is proposed to accommodate this strain. The type strain is SGD-V-25 ( = NCIM 5513 = DSM 28241).

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Funding
This study was supported by the:
  • Council for Scientific and Industrial Research (CSIR)
  • CSIR (Award MLP-027426)
© 2015 IUMS
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2015-05-01
2023-12-01
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Bacillus encimensis sp. nov. isolated from marine sediment
Int J Syst Evol Microbiol 65, 1421 (2015); https://doi.org/10.1099/ijs.0.000114
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