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Abstract

A yellow-pigmented, motile, Gram-stain-negative, moderately halophilic and strictly aerobic bacterium, designated BA42AL-1, was isolated from water of a saltern of Santa Pola, Alicante, Spain. Strain BA42AL-1 grew in media containing 5–20 % (w/v) salts (optimum 7.5 % salts). It grew between pH 6.0 and 9.0 (optimally at pH 7.5) and at 15–45 °C (optimally at 37 °C). Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain BA42AL-1 is a member of the genus . The closest relatives to this strain were YIM 95345 and CG12 with sequence similarities of 99.4 % and 97.0 %, respectively. DNA–DNA hybridization between the novel isolate and YIM 95345 revealed a relatedness of 54 %. The major fatty acids of strain BA42AL-1 were Cω6/Cω7, C cyclo ω and C and lower contents of C and C. The polar lipid pattern of strain BA42AL-1 consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine, two glycolipids, a lipid and four unknown phospholipids. The G+C content of the genomic DNA of this strain was 65.0 mol%. Based on the DNA–DNA hybridization, phenotypic, chemotaxonomic and phylogenetic data presented in this study, strain BA42AL-1 is proposed as a novel species of the genus , for which the name sp. nov. is suggested. The type strain is BA42AL-1 ( = CCM 8472 = CECT 8326 = LMG 27614).

Funding
This study was supported by the:
  • , Spanish Ministry of Science and Innovation , (Award CGL2013-46941-P)
  • , Junta de Andalucía , (Award P10-CVI-6226)
  • , FEDER
  • , Plan Andaluz de Investigación
  • , Spanish Ministry of Science and Innovation
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/content/journal/ijsem/10.1099/ijs.0.000106
2015-04-01
2021-03-07
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