1887

Abstract

A novel endophytic actinomycete, designated strain NEAU-TX2-2, was isolated from moss and characterized using a polyphasic approach. The isolate was found to have morphological characteristics typical of the genus . The isolate formed longitudinally paired spores on the tips of short sporophores that branched from aerial hyphae. Analysis of the 16S rRNA gene sequence supported the assignment of the novel strain to the genus , and strain NEAU-TX2-2 exhibited 99.08 and 98.62 % gene sequence similarities to JCM 3021 and JCM 3006, respectively. However two tree-making algorithms supported the position that strain NEAU-TX2-2 formed a distinct clade with JCM 3006. A low level of DNA–DNA relatedness allowed the isolate to be differentiated from JCM 3021 and JCM 3006. Moreover, strain NEAU-TX2-2 could also be distinguished from its closest phylogenetic relatives by morphological and physiological characteristics. Therefore, it is proposed that strain NEAU-TX2-2 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is NEAU-TX2-2 ( = CGMCC 4.7138 = DSM 46710).

Funding
This study was supported by the:
  • National Outstanding Youth Foundation (Award 31225024)
  • National Key Project for Basic Research (Award 2010CB126102)
  • National Key Technology R&D Program (Award 2012BAD19B06)
  • Program for New Century Excellent Talents in University (Award NCET-11-0953)
  • National Natural Science Foundation of China (Award 31471832, 31071750, 31171913 and 31372006)
  • Outstanding Youth Foundation of Heilongjiang Province (Award JC201201)
  • Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (Award CSCP)
  • Heilongjiang Provincial Educational Commission (Award 12541001)
  • Youth Science Foundation of Heilongjiang Province (Award QC2014C013)
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2015-04-01
2021-08-05
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References

  1. Boondaeng A., Ishida Y., Tamura T., Tokuyama S., Kitpreechavanich V. ( 2009 ). Microbispora siamensis sp. nov., a thermotolerant actinomycete isolated from soil. . Int J Syst Evol Microbiol 59, 31363139. [View Article] [PubMed]
    [Google Scholar]
  2. Collins M. D. ( 1985 ). Isoprenoid quinone analyses in bacterial classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267287. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [View Article] [PubMed]
    [Google Scholar]
  4. Duangmal K., Mingma R., Pathom-aree W., Niyomvong N., Inahashi Y., Matsumoto A., Thamchaipenet A., Takahashi Y. ( 2012 ). Microbispora thailandensis sp. nov., an actinomycete isolated from cave soil. . J Antibiot (Tokyo) 65, 491494. [View Article] [PubMed]
    [Google Scholar]
  5. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  6. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783789. [View Article]
    [Google Scholar]
  7. Gao R. X., Liu C. X., Zhao J. W., Jia F. Y., Yu C., Yang L. Y., Wang X. J., Xiang W. S. ( 2014 ). Micromonospora jinlongensis sp. nov., isolated from muddy soil in China and emended description of the genus Micromonospora . . Antonie van Leeuwenhoek 105, 307315. [View Article] [PubMed]
    [Google Scholar]
  8. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H. N. ( 1974 ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24, 5463. [View Article]
    [Google Scholar]
  9. Hayakawa M., Nonomura H. ( 1987 ). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. . J Ferment Technol 65, 501509. [View Article]
    [Google Scholar]
  10. Huss V. A. R., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [View Article] [PubMed]
    [Google Scholar]
  11. Jones K. L. ( 1949 ). Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. . J Bacteriol 57, 141145.[PubMed]
    [Google Scholar]
  12. Kelly K. L. ( 1964 ). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  13. Kim S. B., Brown R., Oldfield C., Gilbert S. C., Iliarionov S., Goodfellow M. ( 2000 ). Gordonia amicalis sp. nov., a novel dibenzothiophene-desulphurizing actinomycete. . Int J Syst Evol Microbiol 50, 20312036. [View Article] [PubMed]
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721.[PubMed] [CrossRef]
    [Google Scholar]
  15. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  16. Lechevalier M. P., Lechevalier H. A. ( 1980 ). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy (Society for Industrial Microbiology Special Publication no. 6), pp. 227291. Edited by Dietz A., Thayer D. W. . Arlington, VA:: Society for Industrial Microbiology;.
    [Google Scholar]
  17. Lechevalier M. P., De Bièvre C., Lechevalier H. A. ( 1977 ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. . Biochem Syst Ecol 5, 249260. [View Article]
    [Google Scholar]
  18. Mandel M., Marmur J. ( 1968 ). Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12B, 195206. [View Article]
    [Google Scholar]
  19. McKerrow J., Vagg S., McKinney T., Seviour E. M., Maszenan A. M., Brooks P., Seviour R. J. ( 2000 ). A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. . Lett Appl Microbiol 30, 178182. [View Article] [PubMed]
    [Google Scholar]
  20. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. ( 1980 ). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. . J Chromatogr A 188, 221233. [View Article]
    [Google Scholar]
  21. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  22. Miyadoh S., Amano S., Tohyama H., Shomura T. ( 1990 ). A taxonomic review of the genus Microbispora and a proposal to transfer two species to the genus Actinomadura and to combine ten species into Microbispora rosea . . J Gen Microbiol 136, 19051913. [View Article] [PubMed]
    [Google Scholar]
  23. Nakajima Y., Kitpreechavanich V., Suzuki K., Kudo T. ( 1999 ). Microbispora corallina sp. nov., a new species of the genus Microbispora isolated from Thai soil. . Int J Syst Bacteriol 49, 17611767. [View Article] [PubMed]
    [Google Scholar]
  24. Nonomura H., Ohara Y. ( 1957 ). Distribution of actinomycetes in soil. II. Microbispora, a new genus of the Streptomycetaceae . . J Ferment Technol 35, 307311.
    [Google Scholar]
  25. Nonomura H., Ohara Y. ( 1960 ). Distribution of the actinomycetes in soil. IV. The isolation and classification of the genus Microbispora . . J Ferment Technol 38, 401405.
    [Google Scholar]
  26. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  27. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int Syst Bacteriol 16, 313340. [View Article]
    [Google Scholar]
  28. Singh M., Singh S., Nath V., Sahu V., Rawat A. K. ( 2011 ). Antibacterial activity of some bryophytes used traditionally for the treatment of burn infections. . Pharm Biol 49, 526530. [View Article] [PubMed]
    [Google Scholar]
  29. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  30. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  31. Waksman S. A. ( 1961 ). The Actinomycetes. Classification, Identification and Descriptions of Genera and Species, vol. 2. Baltimore, MD:: Williams & Wilkins;.
    [Google Scholar]
  32. Wang X. J., Zhao J. W., Liu C. X., Wang J. D., Shen Y., Jia F. Y., Wang L., Zhang J., Yu C., Xiang W. S. ( 2013 ). Nonomuraea solani sp. nov., an actinomycete isolated from eggplant root (Solanum melongena L.). . Int J Syst Evol Microbiol 63, 24182423. [View Article] [PubMed]
    [Google Scholar]
  33. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  34. Wu C., Lu X., Qin M., Wang Y., Ruan J. ( 1989 ). Analysis of menaquinone compound in microbial cells by HPLC. . Microbiology [English translation of Microbiology (Beijing)] 16 , 176178.
    [Google Scholar]
  35. Xiang W. S., Liu C. X., Wang X. J., Du J., Xi L. J., Huang Y. ( 2011 ). Actinoalloteichus nanshanensis sp. nov., isolated from the rhizosphere of a fig tree (Ficus religiosa) . . Int J Syst Evol Microbiol 61, 11651169. [View Article] [PubMed]
    [Google Scholar]
  36. Xie C. F., Lou H. X. ( 2009 ). Secondary metabolites in bryophytes: an ecological aspect. . Chem Biodivers 6, 303312. [View Article] [PubMed]
    [Google Scholar]
  37. Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G. Z., Chen H. H., Xu L. H., Jiang C. L. ( 2005 ). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55, 11491153. [View Article] [PubMed]
    [Google Scholar]
  38. Xu X. X., Wang H. L., Lin H. P., Wang C., Qu Z., Xie Q. Y., Ruan J. S., Hong K. ( 2012 ). Microbispora hainanensis sp. nov., isolated from rhizosphere soil of Excoecaria agallocha in a mangrove. . Int J Syst Evol Microbiol 62, 24302434. [View Article] [PubMed]
    [Google Scholar]
  39. Yokota A., Tamura T., Hasegawa T., Huang L. H. ( 1993 ). Catenuloplanes japonicas gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales . . Int J Syst Bacteriol 43, 805812. [View Article]
    [Google Scholar]
  40. Zhang Z., Wang Y., Ruan J. ( 1998 ). Reclassification of Thermomonospora and Microtetraspora . . Int J Syst Bacteriol 48, 411422. [View Article] [PubMed]
    [Google Scholar]
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