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Abstract

A Gram-stain-negative, non-motile, rod-shaped bacterial strain, L-1, which was capable of degrading methyl red was isolated from a dye-manufacturing factory in China. Phenotypic, chemotaxonomic and phylogenetic analyses established affiliation of the isolate to the genus . Cells occurred in pairs in young cultures but became chains of coccoid cells in old cultures, and produced a flexirubin-like yellow pigment. Strain L-1 could not hydrolyse cellulose, and had a DNA G+C content of 51.3 mol%. The major cellular fatty acids were iso-C, Cω5, iso-C 3-OH and summed feature 3 (Cω7 and/or Cω6). C, iso-C 3-OH and C 3-OH were the other major fatty acid components. Comparative 16S rRNA gene sequence analysis showed that strainL-1 was most closely related to DSM 18053 (99.2 %), JCM 16232 (98.9 %) and CGMCC 1.6375 (98.7 %). However, the new isolate exhibited relatively low levels of DNA–DNA relatedness with respect to JCM 16232 (41.2±1.8 %), DSM 18053 (38.6±2.6 %) and CGMCC 1.6375 (35.0±2.1 %). Strain L-1 could also be differentiated from its closest phylogenetic relatives based on differences in several phenotypic characteristics. These data suggest that strain L-1 represents a novel species of the genus , for which the name sp. is proposed. The type strain is L-1 (DSM 29057 = CGMCC 1.12969).

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2015-04-01
2020-01-19
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