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Volume 65, Issue Pt_3

gen. nov., sp. nov., isolated from saeu-jeot, traditional Korean fermented shrimp Free

Abstract

A novel Gram-staining-positive, non-endospore-forming, aerobic bacterium, designated strain SJ5-4, was isolated from seau-jeot, a traditional food that is made by fermentation of highly salted [approximately 25 % (w/v)] shrimp in Korea. Cells were moderately halophilic, non-motile cocci or short rods that showed catalase- and oxidase-positive reactions. Growth of strain SJ5-4 was observed at 15–40 °C (optimum, 30 °C), at pH 6.0–9.5 (optimum, pH 6.5–7.0) and in the presence of 1–17 % (w/v) NaCl (optimum, 6 %). The major cellular fatty acids of strain SJ5-4 were anteiso-C, iso-C, anteiso-C, iso-C and C. Phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid, three glycolipids and an unidentified lipid were detected as the polar lipids. Strain SJ5-4 belonged to the exceptionally small group of strains within the order that show a peptidoglycan cross-linked according to the A-type but containing 2,4-diaminobutyric acid. The G+C content of the genomic DNA was 61.8 mol%. MK-7, MK-8 and MK-9 were detected as the isoprenoid quinones. Strain SJ5-4 was most closely related to members of the genus with 16S rRNA gene sequence similarities of 93.1–94.8 %. However, phylogenetic inference based on 16S rRNA gene sequences showed that strain SJ5-4 formed a phyletic lineage distinct from members of the genus within the family . On the basis of its phenotypic and molecular features, strain SJ5-4 represents a novel genus and species of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is SJ5-4 ( = KACC 16909 = JCM 18572 = DSM 28238).

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Funding
This study was supported by the:
  • Technology Development Program for Agriculture and Forestry (TDPAF) of the Ministry for Agriculture, Forestry and Fisheries
  • Program for Collection of Domestic Biological Resources from the National Institute of Biological Resources (Award 2014-02-001)
© 2015 IUMS
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2015-03-01
2024-04-27
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Garicola koreensis gen. nov., sp. nov., isolated from saeu-jeot, traditional Korean fermented shrimp
Int J Syst Evol Microbiol 65, 1015 (2015); https://doi.org/10.1099/ijs.0.000056
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