1887

Abstract

A Gram-negative, oxidase- and catalase-positive bacterium, designated strain EM 4, which varied in shape from rod-shaped to curved or helical with frequently observed bulb-shaped protuberances, was isolated from purified water. 16S rRNA gene sequence analysis indicated that the novel strain belongs to the family within the phylum ; the closest relative among bacterial species with validly published names was determined to be NBRC 103935, with 93.4 % sequence identity. The main fatty acids of strain EM 4 were iso-C, iso-C and iso-C 3-OH. The polar lipid profile consisted of phosphatidylethanolamine, aminolipids, aminophospholipids and unknown lipids; the quinone system consisted of menaquinone MK-7. 16S rRNA gene sequence analysis and the polar lipid and fatty acid profiles suggest that the strain represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is strain EM 4 ( = DSM 25353 = CCUG 62278).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000040
2015-03-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/3/920.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000040&mimeType=html&fmt=ahah

References

  1. An D.-S., Lee H.-G., Im W.-T., Liu Q.-M., Lee S.-T.. ( 2007;). Segetibacter koreensis gen. nov., sp. nov., a novel member of the phylum Bacteroidetes, isolated from the soil of a ginseng field in South Korea. . Int J Syst Evol Microbiol 57:, 1828–1833. [CrossRef][PubMed]
    [Google Scholar]
  2. Anders H., Dunfield P. F., Lagutin K., Houghton K. M., Power J. F., Mackenzie A. D., Vyssotski M., Ryan J. L., Hanssen E. G.. & other authors ( 2014;). Thermoflavifilum aggregans gen. nov., sp. nov., a thermophilic and slightly halophilic filamentous bacterium from the phylum Bacteroidetes. . Int J Syst Evol Microbiol 64:, 1264–1270. [CrossRef][PubMed]
    [Google Scholar]
  3. Bernardet J.-F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  4. Cowan S. T., Steel K. J.. ( 1974;). Manual for Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  5. Fluharty D. M.. ( 1967;). The KOH method for detecting the gram-staining potential of bacteria without staining. . Proc Annu Meet U S Anim Health Assoc 71:, 473–479.[PubMed]
    [Google Scholar]
  6. Hanada S., Tamaki H., Nakamura K., Kamagata Y.. ( 2014;). Crenotalea thermophila gen. nov., sp. nov., a member of the family Chitinophagaceae isolated from a hot spring. . Int J Syst Evol Microbiol 64:, 1359–1364. [CrossRef][PubMed]
    [Google Scholar]
  7. Kämpfer P., Kroppenstedt R. M.. ( 1996;). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42:, 989–1005. [CrossRef]
    [Google Scholar]
  8. Kämpfer P., Lodders N., Falsen E.. ( 2011;). Hydrotalea flava gen. nov., sp. nov., a new member of the phylum Bacteroidetes and allocation of the genera Chitinophaga, Sediminibacterium, Lacibacter, Flavihumibacter, Flavisolibacter, Niabella, Niastella, Segetibacter, Parasegetibacter, Terrimonas, Ferruginibacter, Filimonas and Hydrotalea to the family Chitinophagaceae fam. nov.. Int J Syst Evol Microbiol 61:, 518–523. [CrossRef][PubMed]
    [Google Scholar]
  9. Kim B.-Y., Weon H.-Y., Yoo S.-H., Hong S.-B., Kwon S.-W., Stackebrandt E., Go S.-J.. ( 2007;). Niabella aurantiaca gen. nov., sp. nov., isolated from a greenhouse soil in Korea. . Int J Syst Evol Microbiol 57:, 538–541. [CrossRef][PubMed]
    [Google Scholar]
  10. Kim S. J., Park J. H., Lim J. M., Ahn J. H., Anandham R., Weon H. Y., Kwon S. W.. ( 2014;). Parafilimonas terrae gen. nov., sp. nov., isolated from greenhouse soil. . Int J Syst Evol Microbiol 64:, 3040–3045. [CrossRef][PubMed]
    [Google Scholar]
  11. Leandro T., França L., Nobre M. F., Rainey F. A., da Costa M. S.. ( 2013;). Heliimonas saccharivorans gen. nov., sp. nov., a member of the family Chitinophagaceae isolated from a mineral water aquifer, and emended description of Filimonas lacunae. . Int J Syst Evol Microbiol 63:, 3793–3799. [CrossRef][PubMed]
    [Google Scholar]
  12. Lim J. H., Baek S.-H., Lee S.-T.. ( 2009;). Ferruginibacter alkalilentus gen. nov., sp. nov. and Ferruginibacter lapsinanis sp. nov., novel members of the family ‘Chitinophagaceae’ in the phylum Bacteroidetes, isolated from freshwater sediment. . Int J Syst Evol Microbiol 59:, 2394–2399. [CrossRef][PubMed]
    [Google Scholar]
  13. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S.. & other authors ( 2004;). arb: a software environment for sequence data. . Nucleic Acids Res 32:, 1363–1371. [CrossRef][PubMed]
    [Google Scholar]
  14. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  15. Peplies J., Kottmann R., Ludwig W., Glöckner F. O.. ( 2008;). A standard operating procedure for phylogenetic inference (SOPPI) using (rRNA) marker genes. . Syst Appl Microbiol 31:, 251–257. [CrossRef][PubMed]
    [Google Scholar]
  16. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O.. ( 2007;). silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb. . Nucleic Acids Res 35:, 7188–7196. [CrossRef][PubMed]
    [Google Scholar]
  17. Qu J.-H., Yuan H.-L.. ( 2008;). Sediminibacterium salmoneum gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from sediment of a eutrophic reservoir. . Int J Syst Evol Microbiol 58:, 2191–2194. [CrossRef][PubMed]
    [Google Scholar]
  18. Qu J.-H., Yuan H.-L., Yang J.-S., Li H.-F., Chen N.. ( 2009;). Lacibacter cauensis gen. nov., sp. nov., a novel member of the phylum Bacteroidetes isolated from sediment of a eutrophic lake. . Int J Syst Evol Microbiol 59:, 1153–1157. [CrossRef][PubMed]
    [Google Scholar]
  19. Sangkhobol V., Skerman V. B. D.. ( 1981;). Chitinophaga, a new genus of chitinolytic myxobacteria. . Int J Syst Bacteriol 31:, 285–293. [CrossRef]
    [Google Scholar]
  20. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. . USFCC Newsl 20:, 1–6.
    [Google Scholar]
  21. Shiratori H., Tagami Y., Morishita T., Kamihara Y., Beppu T., Ueda K.. ( 2009;). Filimonas lacunae gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from fresh water. . Int J Syst Evol Microbiol 59:, 1137–1142. [CrossRef][PubMed]
    [Google Scholar]
  22. Skerman V. B. D.. ( 1967;). A Guide to the Identification of the Genera of Bacteria, , 2nd edn.. Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  23. Stamatakis A.. ( 2014;). RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. . Bioinformatics 30:, 1312–1313. [CrossRef][PubMed]
    [Google Scholar]
  24. Stanier R. Y., Palleroni N. J., Doudoroff M.. ( 1966;). The aerobic pseudomonads: a taxonomic study. . J Gen Microbiol 43:, 159–271. [CrossRef][PubMed]
    [Google Scholar]
  25. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  26. Tindall B. J.. ( 1990a;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13:, 128–130. [CrossRef]
    [Google Scholar]
  27. Tindall B. J.. ( 1990b;). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  28. Tindall B. J., Sikorski J., Smibert R. A., Krieg N. R.. ( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, pp. 330–393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R... Washington, DC:: American Society for Microbiology;. [CrossRef]
    [Google Scholar]
  29. Weon H.-Y., Kim B.-Y., Yoo S.-H., Lee S.-Y., Kwon S.-W., Go S.-J., Stackebrandt E.. ( 2006;). Niastella koreensis gen. nov., sp. nov. and Niastella yeongjuensis sp. nov., novel members of the phylum Bacteroidetes, isolated from soil cultivated with Korean ginseng. . Int J Syst Evol Microbiol 56:, 1777–1782. [CrossRef][PubMed]
    [Google Scholar]
  30. Xie C.-H., Yokota A.. ( 2006;). Reclassification of [Flavobacterium] ferrugineum as Terrimonas ferruginea gen. nov., comb. nov., and description of Terrimonas lutea sp. nov., isolated from soil. . Int J Syst Evol Microbiol 56:, 1117–1121. [CrossRef][PubMed]
    [Google Scholar]
  31. Yoon M.-H., Im W.-T.. ( 2007;). Flavisolibacter ginsengiterrae gen. nov., sp. nov. and Flavisolibacter ginsengisoli sp. nov., isolated from ginseng cultivating soil. . Int J Syst Evol Microbiol 57:, 1834–1839. [CrossRef][PubMed]
    [Google Scholar]
  32. Zhang K., Tang Y., Zhang L., Dai J., Wang Y., Luo X., Liu M., Luo G., Fang C.. ( 2009;). Parasegetibacter luojiensis gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from a forest soil. . Int J Syst Evol Microbiol 59:, 3058–3062. [CrossRef][PubMed]
    [Google Scholar]
  33. Zhang N. N., Qu J.-H., Yuan H.-L., Sun Y. M., Yang J.-S.. ( 2010;). Flavihumibacter petaseus gen. nov., sp. nov., isolated from soil of a subtropical rainforest. . Int J Syst Evol Microbiol 60:, 1609–1612. [CrossRef][PubMed]
    [Google Scholar]
  34. Zhang L., Wang Y., Wei L., Wang Y., Shen X., Li S.. ( 2013;). Taibaiella smilacinae gen. nov., sp. nov., an endophytic member of the family Chitinophagaceae isolated from the stem of Smilacina japonica, and emended description of Flavihumibacter petaseus. . Int J Syst Evol Microbiol 63:, 3769–3776. [CrossRef][PubMed]
    [Google Scholar]
  35. Zhao R., Chen X. Y., Li X. D., Tian Y., Kong B. H., Chen Z. L., Li Y. H.. ( 2014;). Cnuella takakiae gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from Takakia lepidozioides. . Int J Syst Evol Microbiol 64:, 607–612. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000040
Loading
/content/journal/ijsem/10.1099/ijs.0.000040
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error