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Abstract
The halophilic archaeal strain, YC82T, was isolated from Yuncheng salt lake in Shanxi, PR China. Cells from strain YC82T were Gram-stain negative, pleomorphic rods, which lysed in distilled water and formed light-red colonies on solid media. Strain YC82T grew at 25–50 °C (optimum 37 °C), in 1.4–4.8 M NaCl (optimum 2.0 M), in 0–1.0 M MgCl2 (optimum 0.05 M) and at pH 6.0–9.5 (optimum pH 7.5). The major polar lipids of strain YC82T were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and three glycolipids, which were chromatographically identical to those of Haloarchaeobius iranensis IBRC-M 10013T and Haloarchaeobius litoreus CGMCC 1.10390T. 16S rRNA gene analysis revealed that strain YC82T had two dissimilar 16S rRNA genes and that it was phylogenetically related to Hab. iranensis IBRC-M 10013T (94.3–99.0 % nucleotide identity) and Hab. litoreus CGMCC 1.10390T (94.1–98.8 % nucleotide identity). The rpoB′ gene similarities between strain YC82T and Hab. iranensis IBRC-M 10013T and Hab. litoreus CGMCC 1.10390T were 96.5 % and 95.7 %, respectively. The DNA G+C content of strain YC82T was 63.7 mol%. Strain YC82T showed low DNA–DNA relatedness with Hab. iranensis IBRC-M 10013T and Hab. litoreus CGMCC 1.10390T. The phenotypic, chemotaxonomic and phylogenetic properties of strain YC82T ( = CGMCC 1.12232T = JCM 18644T) suggest that it represents a novel species of the genus Haloarchaeobius , for which the name Haloarchaeobius salinus sp. nov. is proposed. An emended description of the genus Haloarchaeobius is also presented.
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Funding
- National Natural Science Foundation of China (Award 31370054)
- China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (Award DY125-15-R-03)
- Qinglan Project of Jiangsu Province
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)