Seven strains of three new yeast species were isolated from soil, flowers and leaves in the Nansei Islands, Japan. These isolates most closely resembled in physiological characteristics and nuclear DNA base composition (30–32 mol% G+C), but on the basis of DNA-DNA hybridization and electrophoretic karyotyping they were categorized into three new species different from Phylogenetic analysis using 18S rRNA gene sequence showed that the three new species and were highly related to one another and phylogenetically separate from the members of other species. On the basis of phylogeny and physiological characters, it is proposed that the three new species represent novel taxa and should be designated gen. nov., sp. nov. (type strain IFO 10929), gen. nov., sp. nov. (type strain IFO 10899) and gen. nov., sp. nov. (type strain IFO 10925), while becomes comb. nov.


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