1887

Abstract

Seven strains of three new yeast species were isolated from soil, flowers and leaves in the Nansei Islands, Japan. These isolates most closely resembled in physiological characteristics and nuclear DNA base composition (30–32 mol% G+C), but on the basis of DNA-DNA hybridization and electrophoretic karyotyping they were categorized into three new species different from Phylogenetic analysis using 18S rRNA gene sequence showed that the three new species and were highly related to one another and phylogenetically separate from the members of other species. On the basis of phylogeny and physiological characters, it is proposed that the three new species represent novel taxa and should be designated gen. nov., sp. nov. (type strain IFO 10929), gen. nov., sp. nov. (type strain IFO 10899) and gen. nov., sp. nov. (type strain IFO 10925), while becomes comb. nov.

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1999-10-01
2024-12-06
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References

  1. Cai J., Roberts I. N., Collins M. D. 1996; Phylogenetic relationships among members of the ascomycetous yeast genera Brettanomyces, Debaryomyces, Dekkera, and Kluyveromyces deduced by small-subunit rRNA gene sequences. Im J Syst Bacteriol 46:542–549
    [Google Scholar]
  2. Carle G. F., Olson M. V. 1985; An electrophoretic karyotype for yeast. Proc Natl Acad Sci USA 82:3756–3760
    [Google Scholar]
  3. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
    [Google Scholar]
  4. Fuson G. B., Presley H. L., Phaff H. J. 1987; Deoxyribonucleic acid base sequence relatedness among members of the yeast genus Kluyveromyces. Int J Syst Bacteriol 37:371–379
    [Google Scholar]
  5. Holm C., Meeks-Wagner D. W., Fangman W. L., Botstein D. 1986; A rapid, efficient method for isolating DNA from yeast. Gene 42:169–173
    [Google Scholar]
  6. James S. A., Cai J., Roberts I. N., Collins M. D. 1997; A phylogenetic analysis of the genus Saccharomyces based on 18S rRNA gene sequences: description of Saccharomyces kunashirensis sp. nov. and Saccharomyces martiniae sp. nov. Int J Syst Bacteriol 47:453–460
    [Google Scholar]
  7. Johannsen E. 1980; Hybridization studies within the genus Kluyveromyces van der Walt emend, van der Walt. Antonie Leeuwenhoek 46:177–189
    [Google Scholar]
  8. Kaneko Y., Banno I. 1991; Re-examination of Saccharomyces bayanus strains by DNA-DNA hybridization and electrophoretic karyotyping. Inst Ferment Res Commun 15:30–41
    [Google Scholar]
  9. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120
    [Google Scholar]
  10. Kock J. L. F., Coetzee D. J., Pretorius G. H. J. 1988; The phylogenetic development of species representing the genus Kluyveromyces. Syst Appl Microbiol 10:293–296
    [Google Scholar]
  11. Kudrjanzev V. I. 1960 Lateinische diagnosen der beschriebenen taxonomischen gruppen. Die Systematic der Hefen275 Berlin: Akademie Verlag;
    [Google Scholar]
  12. Lachance M. A. 1998 Kluyveromyces van der Walt emend, van der Walt. The Yeasts, a Taxonomic Study, 4th.227–247 Edited by Kurtzman C. P., Fell J. W. Amsterdam: Elsevier;
    [Google Scholar]
  13. Molnar O., Prillinger H., Lopandic K., Weigang F., Staudacher E. 1996; Analysis of coenzyme Q systems, monosaccharide patterns of purified cell walls, and RAPD-PCR patterns in the genus Kluyveromyces. Antonie Leeuwenhoek 70:67–78
    [Google Scholar]
  14. Nakagiri A. 1991; Coenzyme Q systems in the genus Corol-lospora and allied marine fungi. Inst Ferment Res Commun 15:97–104
    [Google Scholar]
  15. Naumov G. I. 1986; Genosystematics of yeasts of the genus Zygofabospora Kudriavzev emend. G. Naumov. Mol Genet Mikrobiol Virusol 5:10–13 in Russian
    [Google Scholar]
  16. Naumov G. I. 1987; Nomenclature of a yeast genus, Zygofabospora Kudriavzev emend. G. Naumov. Mikol Fitopatol 21:134–140 in Russian
    [Google Scholar]
  17. Poncet S. 1973; Taxonomie numerrique du genre Kluyveromyces. Mycopathol Mycol Appl 51:267–281
    [Google Scholar]
  18. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  20. Shen P., Jong S.-C., Molina F. I. 1994; Analysis of ribosomal DNA restriction patterns in the genus Kluyveromyces. Antonie Leeuwenhoek 65:99–105
    [Google Scholar]
  21. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128
    [Google Scholar]
  22. Vaughan-Martini A., Martini A. 1987; Taxonomic revision of the yeast genus Kluyveromyces by nuclear deoxyribonucleic acid reassociation. Int J Syst Bacteriol 37:380–385
    [Google Scholar]
  23. Vaughan-Martini A., Martini A., Cardinali G. 1993; Electrophoretic karyotyping as a taxonomic tool in the genus Saccharomyces. Antonie Leeuwenhoek 63:145–156
    [Google Scholar]
  24. van der Walt J. P. 1963; Fabospora phaffii sp. n. Antonie Leeuwenhoek 29:319–322
    [Google Scholar]
  25. van der Walt J. P. 1965; The emendation of the genus Kluyveromyces v. d. Walt. Antonie Leeuwenhoek 31:341–348
    [Google Scholar]
  26. van der Walt J. P. 1971; New combinations in the genera Brettanomyces, Kluyveromyces, Lodderomyces and Wingea. Bothalia 10:417–418
    [Google Scholar]
  27. van der Walt J. P., Yarrow D. 1984 Methods for isolation, maintenance, classification and identification of yeasts. The Yeasts, a Taxonomic Study, 3rd.76–97 Edited by Kreger-van Rij N. J. W. Amsterdam: Elsevier;
    [Google Scholar]
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