A new, mesophillic, facultatively anaerobic, psychrotolerant bacterium, strain ANG-SQ1 (T=type strain), was isolated from a microbial community colonizing the accessory nidamental gland of the squid . It was selected from the community on the basis of its ability to reduce elemental sulfur. The cells are motile, Gram-negative rods (2·0–3·0 μm long, 0·4–0·6 μm wide). ANG-SQ1 grows optimally over the temperature range of 25–30 °C and a pH range of 6·5–7·5 °C in media containing 0·5 M NaCl. 16S rRNA sequence analysis revealed that this organism belongs to the γ-3 subclass of the . The closest relative of ANG-SQ1 is , with a 16S rRNA sequence similarity of 97·0%. Growth occurs with glucose, lactate, acetate, pyruvate, glutamate, citrate, succinate, Casamino acids, yeast extract or peptone as sole energy source under aerobic conditions. The isolate grows anaerobically by the reduction of iron, manganese, nitrate, fumarate, trimethylamine--oxide, thiosulfate or elemental sulfur as terminal electron acceptor with lactate. Growth of ANG-SQ1 was enhanced by the addition of choline chloride to growth media lacking Casamino acids. The addition of leucine or valine also enhanced growth in minimal growth media supplemented with choline. The results of both phenotypic and genetic characterization indicate that ANG-SQ1 is a species. Thus it is proposed that this new isolate be assigned to the genus and that it should be named sp. nov., in recognition of its association with .


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error