1887

Abstract

A strictly anaerobic, halotolerant, spindle-shaped rod, designated strain SEBR 4211, was isolated from an African saline oil-producing well. Cells stain Grampositive, which was confirmed by electron microscopy observations. Strain SEBR 4211 was motile by means of one to four peritrichous flagella, had a G+C content of 43 mol% and grew optimally at 37 °C, pH 7·3, with 0 to 3% (w/v) NaCI. It utilized a limited number of carbohydrates (cellobiose, glucose, fructose, mannitol and ribose) and produced acetate, butyrate, CO and H as end products from glucose fermentation. It reduced thiosulfate to sulfide. In the presence of thiosulfate, a decrease in butyrate and an increase in acetate production was observed. Phylogenetically, strain SEBR 4211 was related to members of the low G+C order with as the closest relative (16S rDNA sequence similarity of 90%). On the basis of phenotypic, genotypic and phylogenetic characteristics of the isolate, it is proposed to designate it as a new species of a new genus, gen. nov., as sp. nov. The type strain is SEBR 4211 (= DSM 12116

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/content/journal/ijsem/10.1099/00207713-49-3-1141
1999-07-01
2019-11-23
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http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-49-3-1141
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