1887

Abstract

A Gram-positive, anaerobic, moderate thermophile, strain Wv6, capable of reducing indigo dye, was isolated from a fermenting woad vat prepared essentially as in medieval Europe. Strain Wv6 formed rod-shaped cells, which occurred singly, in pairs or in chains and produced terminal oval endospores Strain Wv6 was saccharolytic. Growth occurred at pH 5.9-9.9 (initial pH) with an optimum at 50 °C of pH 7.2±0.2 (constant pH). At pH 7.8, the temperature range for growth was 30-55 °C with the optimum at 49-52 °C. Comparative 16S rRNA gene sequence analysis demonstrated that the bacterium represents a hitherto unknown subline within rRNA cluster I . Based on the results of the phylogenetic analysis and phenotypic criteria, it is proposed that the unknown moderate thermophile should be classified as sp. nov., a new species of the genus . The type strain of is strain Wv6 (= NCFB 3071).

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/content/journal/ijsem/10.1099/00207713-49-3-1025
1999-07-01
2019-11-13
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http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-49-3-1025
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