A novel pathogen from Asian pears ( Nakai) was analysed by sequencing the 16S rDNA and the adjacent intergenic region, and the data were compared to related . The 16S rDNA of the Asian pear pathogen was almost identical with the sequence of , in contrast to the 16S--23S rRNA intergenic transcribed spacer region of both species. A dendrogram was deduced from determined sequences of the spacer regions including those of several related species such as subsp. stewartii and . Dendrograms derived from 121 biochemical characteristics including Biotype 100 data placed the Asian pear pathogen close to and more distantly to other members of the species and to the species and . Another DNA relatedness study was performed by DNA hybridizations and estimation of Δ values. The Asian pear strains constituted a tight DNA hybridization group (89--100%) and were barely related to strains of (40–50%) with a Δ in the range of 5·2--6·8. The G+C content of DNA from the novel pathogen is 52 mol %. Therefore, it is proposed that strains isolated from Asian pears constitute a new species and the name is suggested; the type strain is strain Ep16/96(= CFBP 4172= DSM 12163).


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