1887

Abstract

Thermophilic streptococci were isolated from biofilms on stainless steel samples exposed to pasteurized skimmed milk and from dairy products from a dairy manufacturing plant. The phenotypic characters of these isolates were distinct from those of other thermophilic streptococci of dairy origin ( and ). Genotypic data [restriction endonuclease analysis, ribotyping, random amplified polymorphic DNA (RAPD) profiles, DNA-DNA hybridization and G+C contents] support the classification of these isolates as a new species. The sequence of the 16S rRNA was compared with that of 29 species of streptococci and shown to be significantly different. The sequence of the 16S-23S rRNA intergenic spacer region also differed from published sequences of closely related species. A fluorescent hybridization probe prepared to a specific part of the 16S rRNA gene sequence was able to distinguish the unknown isolates from reference isolates of and . It is proposed that these thermophilic streptococcal isolates from a dairy environment be classified in the genus as a new species, (from waiu, the New Zealand Maori word for milk). The type strain is 3/1(= NZRCC 20100).

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1999-04-01
2022-12-01
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References

  1. Bentley R. W., Leigh J. A., Collins M. D. 1991; Intrageneric structure of Streptococcus based on comparative analysis of small-subunit rRNA sequences.. Int J Syst Bacteriol 41:487–494
    [Google Scholar]
  2. Bridge P. D., Sneath P. H. A. 1983; Numerical taxonomy of Streptococcus . J Gen Microbiol 129:565–597
    [Google Scholar]
  3. Coykendell A. L., Gustafson K. B. 1985; Deoxyribonucleic acid hybridisations among strains of Streptococcus salivarius and Streptococcus bovis . Int J Syst Bacteriol 35:274–280
    [Google Scholar]
  4. Driessen F. M., Bouman S. 1979; Growth of thermoresistant streptococci in cheese milk pasteurizers - trials with a model pasteurizer.. Zuivelzicht 71:1062–1064
    [Google Scholar]
  5. Farrow J. A. E., Collins M. D. 1984; DNA base composition, DNA-DNA homology and long-chain fatty acid studies on Streptococcus thermophilus and Streptococcus salivarius . J Gen Microbiol 130:357–362
    [Google Scholar]
  6. Farrow J. A. E., Kruze J., Phillips B. A., Bramley A. J., Collins M. D. 1984; Taxonomic studies on Streptococcus bovis and Streptococcus equinus : description of Streptococcus alactolyticus sp. nov. and Streptococcus saccharolyticus sp. nov.. Syst Appl Microbiol 5:467–482
    [Google Scholar]
  7. Felsenstein J. 1989; phylip - phylogeny inference package (version 3.2).. Cladistics 5:164–166
    [Google Scholar]
  8. Flint S. H., Bremer P. J., Brooks J. B. 1997; Biofilms in dairy manufacturing plant - description, current concerns and methods of control.. Biofouling 11:81–97
    [Google Scholar]
  9. Hardie J. M. 1986; Genus Streptococcus. . Bergey’s Manual of Systematic Bacteriology,1069 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  10. Hardie J. M., Wiley R. A. 1994; Recent developments in streptococcal taxonomy : their relation to infections.. Rev Med Microbiol 5:151–162
    [Google Scholar]
  11. Maidak B., Olsen G., Larsen N., Overbeek R., McCaughey M., Woese C. 1997; The RDP (Ribosomal Database Project).. Nucleic Acids Res 25:109–111
    [Google Scholar]
  12. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms.. J Mol Biol 3:208–218
    [Google Scholar]
  13. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.. J Mol Biol 5:109–118
    [Google Scholar]
  14. Nicholas K. B., Nicholas H. B. Jr 1997; Genedoc: a tool for editing and annotating multiple sequence alignments. . Distributed by the authors www.cris.com/~ketchup/genedoc.shtml
  15. Poulsen L. K. 1995; Protocol for the Use of Fluorescently Labelled Oligonucleotide Probes - Determination of Bacterial Identity, Activity and Gene Expression. . Lyngby: Department of Microbiology, The Technical University of Denmark;
    [Google Scholar]
  16. Schleifer K. H., Ehrmann M., Krusch U., Neve H. 1991; Revival of the species Streptococcus thermophilus (ex. Orla-Jenson, 1919) nom. rev.. Syst Appl Microbiol 14:386–388
    [Google Scholar]
  17. Schleifer K.-H., Ehrmann M., Beimfohu C., Brockmann E., Ludwig W., Amann R. 1995; Application of molecular methods for the classification and identification of lactic acid bacteria.. Int Dairy J 5:1081–1094
    [Google Scholar]
  18. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis.. J Mol Biol 98:503–517
    [Google Scholar]
  19. Thompson J. D., Gobson T. J., Plewniak F., Jeanmougin F., Hiffins D. G. 1997; The clustal-x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.. Nucleic Acids Res 25:4876–4882
    [Google Scholar]
  20. Wood B. J. B., Holzapfel W. H. 1995; The Genera of Lactic Acid Bacteria. . Glasgow: Blackie;
    [Google Scholar]
  21. Young J. P. W., Downer H. J., Eardly B. D. 1991; Phylogeny of the prototrophic Rhizobium strain BTAil by polymerase chain reaction-based sequencing of a 16S rRNA gene segment.. J Bacteriol 143:2271–2277
    [Google Scholar]
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