The taxonomic status of an actinomycete (MA140/96) isolated from indoor building materials of a children's day care centre was studied using the polyphasic approach. The cell morphology was atypical for an actinomycete, electron microscopy revealed a hairy surface, highly unusual for Gram-positi bacteria. The organisms grew at 10--37°C no growth was visible at 5 °C and 45 °C in 5 d. The cell wall contained the diamino acid -diaminopimelic acid and the sugars arabinose, galactose, mannose and ribose. The phospholipids phosphatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and diphosphatidylglycerol were detected. The only menaquinone found was MK9(H). The fatty acid pattern was composed of palmitic acid (23.6%) palmitole acid (16.5%) and another hexadecenoic acid 16:1cis11 (1.4%), oleic acid (29.9%), stearic acid (2.9%) and the 10-methyl-branched tuberculostearic acid (23.2%). A gas-chromatographic analysis of the mycolic acid revealed a carbo chain length of C-CThe G+C was 64.8 mol%. The results of 16S rDNA sequence comparisons revealed that strain MA140/96represents a new lineage in the suborder of the order . Therefore, it was concluded that strain MA140/96should be assigned to a new genus and species, for which the name gen. nov., sp. nov. proposed. The type strain of the species is MA140/96(= DSM 44343).


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