1887

Abstract

The taxonomic status of an actinomycete (MA140/96) isolated from indoor building materials of a children’s day care centre was studied using the polyphasic approach. The cell morphology was atypical for an actinomycete, electron microscopy revealed a hairy surface, highly unusual for Gram-positive bacteria. The organisms grew at 10–37 °C, no growth was visible at 5 °C and 45 °C in 5 d. The cell wall contained the diamino acid meso-diaminopimelic acid and the sugars arabinose, galactose, mannose and ribose. The phospholipids phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and diphosphatidylglycerol were detected. The only menaquinone found was MK-9(H). The fatty acid pattern was composed of palmitic acid (23·6%) palmitoleic acid (16·5%) and another hexadecenoic acid 16:111 (1·4%), oleic acid (29·9%), stearic acid (2·9%) and the 10-methyl-branched tuberculostearic acid (23·2%). A gas-chromatographic analysis of the mycolic acid revealed a carbon-chain length of C–C. The G+C was 64·8 mol%. The results of 16S rDNA sequence comparisons revealed that strain MA140/96 represents a new lineage in the suborder of the order . Therefore, it was concluded that strain MA140/96 should be assigned to a new genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of the species is MA140/96 (= DSM 44343).

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1999-04-01
2023-02-06
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