1887

Abstract

Phenotypic and phylogenetic studies were performed with two strains (OCh 239 and OCh 210, T = type strain) of aerobic bacteriochlorophyll-containing bacteria isolated from the charophytes and the epiphytes on the stromatolites, respectively, of a saline lake located on the west coast of Australia. Both strains were chemoheterotrophic, Gram-negative and motile rods with subpolar flagella. Catalase and oxidase were produced. ONPG reaction was positive. Cells utilized -glucose, acetate, butyrate, citrate, -lactate, -malate, pyruvate, succinate, -aspartate and -glutamate. Acids were produced from -fructose and -glucose. Bacteriochlorophyll a was synthesized under aerobic conditions. Strain OCh 239 had nitrate reductase and phosphatase. Acids were produced from -arabinose, -galactose, lactose, maltose, -ribose and sucrose. The strain could grow in 0·20–0% (w/v) NaCI. Strain OCh 210 had urease. Hydrolysis of gelatin was positive. Acids were produced from -xylose. The strain could grow in 0·5–20·0% (w/v) NaCI. The results of 16S rRNA sequence comparisons revealed that strains OCh 239 and OCh 210 formed a new cluster within the -3 group of the subclass of the class . The similarity value of the 16S rRNA sequences between strains OCh 239 and OCh 210 was 95·8%. Therefore, it was concluded that these two strains should be placed in a new genus, gen. nov., as the new species sp. nov. and sp. nov. The type species of the genus is . The type strains of and are OCh 239 (= JCM 10272) and OCh 210 (= JCM 10271), respectively.

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1999-04-01
2024-07-23
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References

  1. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad SciUSA 75:4801–4805
    [Google Scholar]
  2. Evans W. R., Feischman D. E., Calvert H. E., Pyati P. V., Alter G. M., Rao N. S. S. 1990; Bacteriochlorophyll and photosynthetic reaction centers in Rhizobium strain BTAi 1. Appl Environ Microbiol 56:3445–3449
    [Google Scholar]
  3. Felsenstein J. 1995; phylip (phylogeny inference package). version 3.57c Seattle: University of Washington;
    [Google Scholar]
  4. Fuerst J. A., Hawkins J. A., Holmes A., Sly L. I., Moore C. J., Stackebrandt E. 1993; Porphyrobacter neustonensis gen. nov., sp. nov., an aerobic bacteriochlorophyll-synthesizing budding bacterium from fresh water. Int J Syst Bacteriol 43:125–134
    [Google Scholar]
  5. Green P. N., Bousfield I. J. 1983; Emendation of Methylobacterium Patt, Cole, and Hanson 1976; Methylobacterium rhodinum (Heumann 1962) comb. nov. corrig. ; and Methylobacterium radiotolerans (Ito and Iizuka 1971) comb. nov. corrig.; and Methylobacterium mesophilicum (Austin and Goodfellow 1979) comb. nov. Int J Syst Bacteriol 33:875–877
    [Google Scholar]
  6. Hanada S., Kawase Y., Hiraishi A., Takaichi S., Matsuura K., Shimada K., Nagashima K. V. P. 1997; Porphyrobacter tepidarius sp. nov., a moderately thermophilic aerobic photosynthetic bacterium isolated from a hot spring. Int J Syst Bacteriol 47:408–413
    [Google Scholar]
  7. Nishimura Y., Shimadzu M., Iizuka H. 1981; Bacteriochlorophyll formation in radiation-resistant Pseudomonas radiora. J Gen Appl Microbiol 27:427–430
    [Google Scholar]
  8. Nishimura Y., Muroga Y., Saito S., Shiba T., Takamiya K., Shioi Y. 1994; DNA relatedness and chemotaxonomic feature of aerobic bacteriochlorophyll-containing bacteria isolated from coasts of Australia. J Gen Appl Microbiol 40:287–296
    [Google Scholar]
  9. Saito S., Suzuki T., Nishimura Y. 1998; Proposal of Craurococcus roseus gen. nov., sp. nov. and Paracraurococcus ruber sp. nov., novel aerobic bacteriochlorophyll a-containing bacteria from soil. Int J Syst Bacteriol 48:1043–1047
    [Google Scholar]
  10. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning : a Laboratory Manual, 2nd. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Sato K. 1978; Bacteriochlorophyll formation by facultative methylotrophs, Protaminobacter ruber and Pseudomonas AM 1. FEBS Lett 85:207–210
    [Google Scholar]
  12. Shiba T. 1991; Roseobacter litoralis gen. nov., sp. nov. and Roseobacter denitrificans sp. nov., aerobic pink-pigmented bacteria which contain bacteriochlorophyll a. Syst Appl Microbiol 14:140–145
    [Google Scholar]
  13. Shiba T., Simidu U. 1982; Erythrobacter longus gen. nov., sp. nov., an aerobic bacterium which contains bacteriochlorophyll a. Int J Syst Bacteriol 32:211–217
    [Google Scholar]
  14. Shiba T., Simidu U., Taga N. 1979; Distribution of aerobic bacteria which contain bacteriochlorophyll a. Appl Environ Microbiol 38:43–45
    [Google Scholar]
  15. Shiba T., Shioi Y., Takamiya K., Sutton D. C., Wilkinson C. R. 1991; Distribution and physiology of aerobic bacteria containing bacteriochlorophyll a on the east and west coasts of Australia. Appl Environ Microbiol 57:295–300
    [Google Scholar]
  16. Suzuki T., Yamasato K. 1994; Phylogeny of spore-forming lactic acid bacteria based on 16S rRNA gene sequences. FEMS Microbiol Lett 115:13–18
    [Google Scholar]
  17. Taga N. 1968; Some ecological aspects of marine bacteria in the kuroshio current. Bull Misaki Mar Biol Inst Kyoto Univ 12:56–76
    [Google Scholar]
  18. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w : improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
    [Google Scholar]
  19. Wakao N., Shiba T., Hiraishi A., Ito M., Sakurai Y. 1993; Distribution of bacteriochlorophyll a in species of the genus Acidiphilium. Curr Microbiol 11:277–279
    [Google Scholar]
  20. Yurkov V., Beatty J. T. 1998; Isolation of aerobic anoxygenic photosynthetic bacteria from black smoker plume waters of the Juan de Fuca Ridge in the Pacific Ocean. Appl Environ Microbiol 64:337–341
    [Google Scholar]
  21. Yurkov V., Stackebrandt E., Holmes A. 7 other authors 1994; Phylogenetic positions of novel aerobic, bacteriochlorophyll a-containing bacteria and description of Roseococcus thiosulfatophilus gen. nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov. Int J Syst Bacteriol 44:427–134
    [Google Scholar]
  22. Yurkov V., Stackebrandt E., Buss O., Vermeglio A., Gorlenko V., Beatty J. T. 1997; Reorganization of the genus Erythromicrobium: description of “Erythromicrobium sibiricum” as Sandaracinobacter sibiricus gen. nov., sp. nov., and of “Erythromicrobium ursincola” as Erythromonas ursincola gen. nov., sp. nov. Int J Syst Bacteriol 47:1172–1178
    [Google Scholar]
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