1887

Abstract

A novel Treponema species was isolated from an ulcerative lesion of a cow suffering from digital dermatitis (DD), a disease which causes painful ulcerations along the coronary band. Among other anaerobic bacteria, high numbers of spirochaetes have been regularly found in DD lesions. Here data are presented of a spirochaete isolated from a DD ulcer. By chemotaxonomy, protein analysis and comparative 16S rDNA sequence analysis this isolate was classified as a treponeme that differed from all species described previously. The only isolate, DD5/3, for which the name is proposed, is designated the type strain of the novel species. The strain is a small, highly motile spirochaete that has two periplasmic flagella, one flagellum being attached at each cell pole. Strain DD5/3 exhibits -glucosidase and -acetyl--glucosaminidase activity and growth is inhibited by rabbit serum. . was phylogenetically most closely related (89.5 % 16S rRNA similarity) to Treponema maltophilum, an oral spirochaete isolated from a periodontitis patient.

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1999-01-01
2023-01-29
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References

  1. Blowey R. W., Sharp M. W. 1988; Digital dermatitis in dairy cattle. Vet Rec 122:505–508
    [Google Scholar]
  2. Cheli R., Mortellaro C. 1974 La dermatide digitale del bovinoProceedings of the 8th International Conference on Diseases of CattleMilan208–213
    [Google Scholar]
  3. Choi B.-K., Paster B. J., Dewhirst F. E., Göbel U. B. 1994; Diversity of cultivable and uncultivable oral spirochetes from a patient with severe destructive periodontitis. Infect Immun 62:1889–1895
    [Google Scholar]
  4. Choi B.-K., Nattermann H., Grund S., Haider W., Göbel U. B. 1997; Spirochetes from digital dermatitis lesions in cattle are closely related to treponemes associated with human periodontitis. Int J Syst Bacteriol 47:175–181
    [Google Scholar]
  5. Collighan R. J., Woodward M. J. 1997; Spirochaetes and other bacterial species associated with bovine digital dermatitis. FEMS Microbio Lett 156:37–41
    [Google Scholar]
  6. Done S. H., Blowey R. W., Cooley W. 1993 The pathology of digital dermatitis in cattle in the UKProceedings of the American Association of Veterinary Laboratory Diagnosticians MeetingLas Vegas, NV, USA69
    [Google Scholar]
  7. Döpfer D., Koopmans A., Meijer F. A. 7 other authors 1997; Histological and bacteriological evaluation of digital dermatitis in cattle, with special reference to spirochaetes and Campylobacter faecalis. Vet Rec 140:620–623
    [Google Scholar]
  8. Gersdorf H., Pelz K., Göbel U. B. 1993; Fluorescence in situ hybridization for direct visualisation of Gram-negative anaerobes in subgingival plaque samples. FEMS Immunol Med Microbiol 6:109–114
    [Google Scholar]
  9. Grund S., Nattermann H., Horsch F. 1995; Zum elektronenmikroskopischen Spirochäten-Nachweis bei der Dermatitis digitalis des Rindes. J Vet Med 42:533–542
    [Google Scholar]
  10. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism21–132 Munro H. N. New York: Academic Press;
    [Google Scholar]
  11. Kelley R. O., Dekker R. A. F., Bluemink J. G. 1973; Ligand-mediated osmium binding: its application in coating biological specimens for scanning electron microscopy. J Ultrastruct Res 45:254–258
    [Google Scholar]
  12. Kimura Y., Takahashi M., Matsumoto N. 8 other authors 1993; Verrucose dermatitis and digital papillomatosis in dairy cows. J Vet Med Jpn 46:899–906
    [Google Scholar]
  13. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  14. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sog in M. L., Pace N. R. 1985 Rapid determination of 16S ribosomal RNA sequences for phylogenetic analysisProc Natl Acad SeiUSA 82:6955–6959
    [Google Scholar]
  15. Leschine S. B., Canale-Parola E. 1980; Rifampicin as a selective agent for isolation of oral spirochetes. J Clin Microbiol 12:792–795
    [Google Scholar]
  16. Moter A., Hoenig C., Choi B.-K., Riep B., Göbel U. B. 1998; Molecular epidemiology of oral treponemes in periodontal disease. J Clin Microbiol 36:1399–1403
    [Google Scholar]
  17. Nattermann H., Grund S., Ehrlich M., Zucker B., Choi B.-K., Göbel U. B. 1996; Zur Ätiologie der Dermatitis Digitalis des Rindes. In Tagung der Deutschen Vet Gesellschaft, Fachgruppe Bakteriologie und bakterielle Krankheiten345–351
    [Google Scholar]
  18. Nermut M. V. 1973; Methoden der Negativ-Kontrastierung. In Methodensammlung der Elektronenmikroskopie1–18 Schimmel G., Vogell W. Stuttgart: Wisseuschaftlicher;
    [Google Scholar]
  19. van de Peer Y., de Wachter R. 1993; treecon: a software package for the construction and drawing of evolutionary trees. Comput Appl Biosci 9177–182
    [Google Scholar]
  20. Read D. H., Walker R. L., Castro A. E., Sundberg J. P., Thurmond M. C. 1992; An invasive spirochete associated with interdigital papillomatosis of dairy cattle. Vet Rec 130:59–60
    [Google Scholar]
  21. Rijpkema S. G. T., David G. P., Hughes S. L., Woodward M. J. 1997; Partial identification of spirochaetes from two dairy cows with digital dermatitis by polymerase chain reaction analysis of the 16S ribosomal RNA gene. Vet Rec 140:257–259
    [Google Scholar]
  22. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  23. Walker R. L., Read D. H., Loretz K. J., Nordhausen R. W. 1995; Spirochetes isolated from dairy cattle with papillomatous digital dermatitis and interdigital dermatitis. Vet Microbiol 47:343–355
    [Google Scholar]
  24. Walker L. R., Read D. H., Loretz K. J., Hird D. W., Berry S. L. 1997; Humoral response of dairy cattle to spirochetes isolated from papillomatous digital dermatitis lesions. Am J Vet Rec 58:744–748
    [Google Scholar]
  25. Wecke J., Wolf V., Fath S., Bernimoulin J.-P. 1995; The occurrence of treponemes and their spherical bodies on polytetrafiuoroethylene membranes. Oral Microbiol Immunol 10:278–283
    [Google Scholar]
  26. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
  27. Wolf V., Wecke J. 1994; Formation of multiple treponemes. Zentbl Bakteriol 280:297–303
    [Google Scholar]
  28. Wolf V., Lange R., Wecke J. 1993; Development of quasimulticellular bodies of Treponema denticola. Arch Microbiol 160:206–213
    [Google Scholar]
  29. Wyss C. 1992; Growth of Porphyromonas gingivalis Treponema denticola T pectinovorum T socransckii and T vincentii a chemically defined medium. J Clin Microbiol 30:2225–2229
    [Google Scholar]
  30. Wyss C., Choi B.-K., Schupbach P., Guggenheim B., Göbel U. B. 1996; Treponema maltophilum sp. nov., a small oral spirochete isolated from human periodontal lesions. Int J Syst Bacteriol 46:745–752
    [Google Scholar]
  31. Wyss C., Choi B.-K., Schlipbach P., Guggenheim B., Göbel U. B. 1997; Treponema amylovorum sp. nov., a saccharolytic spirochete of medium size isolated from an advanced human periodontal lesion. Int J Syst Bacteriol 47:842–845
    [Google Scholar]
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