Sequencing of gene fragments of reference strains of serovars 1, 3, 6, 14 and 8, in addition to 33 clinical isolates is reported. A phylogenetic tree deduced from alignment of these sequences clearly demonstrates two major clusters (confidence limit 100%), which equate to the parvo and T960 biovars, and five types, which have been designated 1, 3, 6, 8 and X. These relationships are supported by bootstrap analysis. Polymorphisms within the fragment of types 1, 3 and 6 were used to define nine subtypes ( 1a, 1b, 3a, 3b, 3c, 3d, 3e, 6a and 6b), thus facilitating high resolution typing of Inclusion of reference strains for serovars 1, 3, 6 and 8 in the typing scheme showed that the results of this analysis are broadly consistent with currently accepted serotyping. In addition, a gene fragment from nine of the clinical isolates was amplified and sequenced. Comparisons of the sequences clearly distinguished the two biovars of ; however, this fragment was invariant within the parvo biovar. This study has shown that the sequence can reveal the fine details of the relationships between isolates and also supports the significant evolutionary gap between the two biovars.


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