1887

Abstract

The species was proposed to accommodate a heterogeneous group of streptococci associated with infections in animals and human beings. This taxon is now considered to include animal isolates of -haemolytic group C streptococci, previously called . ; animal and human isolates of -haemolytic group C streptococci, previously called ‘. ’; -haemolytic group L strains associated with infections in animals and, rarely, in humans; and -hiaemolytic group G strains isolated from humans. DNA-DNA reassociation experiments (hydroxyapatite method) and multilocus enzyme electrophoresis (MEE) were performed on reference strains and clinical isolates to determine the genetic relationships among these different phenotypic categories. DNA-DNA hybridization tests showed that they were related at the species level, despite the phenotypic and host heterogeneity. Both genotypic and phenotypic characterization indicated that . could be separated into two major sub-groups. The first subgroup contained -haemolytic strains that showed levels of DNA relatedness with the type strain of . ranging from 84 to 90% and from 82 to 88% under optimal (55 °C) and stringent (70 °C) conditions, respectively. The second sub-group contained haemolytic strains showing levels of relatedness ranging from 71 to 79% (55 °C) and from 62 to 73% (70 °C). Percentage divergence varied from 0·5 to 1·0% (-haemolytic group) and from 20 to 3·5% (-haemolytic group). A dendrogram based on phenotypic similarity between the enzyme bands produced by MEE showed a Jaccard similarity coefficient of 0·45 between the subclusters formed by the two sub-groups. The results of phenotypic and genotypic characterization were consistent with a published proposal to divide . into two subspecies, . subsp. and . subsp. , with a few modifications.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-48-4-1231
1998-10-01
2022-10-02
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/48/4/ijs-48-4-1231.html?itemId=/content/journal/ijsem/10.1099/00207713-48-4-1231&mimeType=html&fmt=ahah

References

  1. Balmelli T., Piffaretti J. 1996; Analysis of the genetic polymorphism of Borrelia burgdorferi sensu latu by multilocus enzyme electrophoresis. Int J Syst Bacteriol 46:167–172
    [Google Scholar]
  2. Beebe J. L., Koneman E. W. 1995; Recovery of uncommon bacteria from blood : association with neoplastic disease. Clin Microbiol Rev 8:336–356
    [Google Scholar]
  3. Bert F., Picard B., Lambert-Zechovsky N., Goullet P. 1995; Identification and typing of pyogenic streptococci by enzyme electrophoretic polymorphism. J Med Microbiol 42:442–451
    [Google Scholar]
  4. Bjorck L., Kronvall G. 1984; Purification and some properties of streptococcal protein G, a novel IgG-binding reagent. J Immunol 133:969–973
    [Google Scholar]
  5. Brenner D. J., McWhoter A. C., Leete Knutson J. K., Steigerwalt A. G. 1982; Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds. J Clin Microbiol 15:1133–1140
    [Google Scholar]
  6. Bucher A., Gaustad P. 1990; Septicemia and endocarditis caused by group G streptococci in a Norwegian hospital. Eur J Microbiol Infect Dis 9:251–256
    [Google Scholar]
  7. Carmeli Y., Schapiro J. M., Neeman D., Yinnon A. M., Alkan M. 1995; Streptococci group C bacteremia. Arch Intern Med 155:1170–1176
    [Google Scholar]
  8. Cimolai N., Mah D. 1991; β d-Glucuronidase activity assay for rapid differentiation of species within β-hiaemolytic groups C and G streptococci. J Clin Pathol 44:824–825
    [Google Scholar]
  9. Cimolai N., Morrison B. J., MacCulloch L., Smith D. F., Hlady J. 1991; β-Haemolytic non group A streptococci and pharyngitis: a case control study. Eur J Pediatr 150:776–779
    [Google Scholar]
  10. Cupolillo E., Grimaldi G. Jr, Momen H. 1994; A general classification of New World Leishmania using numerical zymo-taxonomy. Am J Trop. Med Hyg 50:296–311
    [Google Scholar]
  11. Devriese L. A., Hommez J., Kilpper-Balz R., Schleifer K.-H. 1986; Streptococcus canis sp. nov. : a species of group G streptococci from animals. Int J Syst Bacteriol 36:422–425
    [Google Scholar]
  12. Efstratiou A., Colman G., Hahn G., Timoney J. F., Boeufgras J. M., Monget D. 1994; Biochemical differences among human and animal streptococci of Lancefield group C or group G. J Med Microbiol AY145–148
    [Google Scholar]
  13. Ezaki T., Facklam R., Takeuchi N., Yabuuchi E. 1986; Genetic relatedness between the type strain of Streptococcus anginosus and minute-colony-forming beta-hemolytic streptococci carrying different Lancefield grouping antigens. Int J Syst Bacteriol 36:345–347
    [Google Scholar]
  14. Facklam R. R., Washington J. A. II 1991; Streptococcus and related catalase-negative gram-positive cocci. In Manual of Clinical Microbiology238–257 Balows A., Hausler W. J. Jr, Hermann K. L., Isermberg H. D., Shadomy H. J. Washington: DC : American Society for Microbiology;
    [Google Scholar]
  15. Farrow J. A. E., Collins M. D. 1984; Taxonomic studies on streptococci of serological groups C, G and L and possibly related taxu. Syst Appl Microbiol 5:438–493
    [Google Scholar]
  16. Garvie E. I., Farrow J. A. E., Bramley A. J. 1983; Streptococcus dysgalactiae (Diernhofer) nom. rev. Int J Syst Bacteriol 33:404–405
    [Google Scholar]
  17. Harris H., Hopkinson D. A. 1976; The enzymes. In Handbook of Enzyme Electrophoresis in Human Genetics, chapter 4 New York: New York Science Publishers;
    [Google Scholar]
  18. Holt J. G., Krieg N. R., Sneath P. H. A., Stanley J. T., Willians S. T. 1994; Gram-positive cocci. In Bergey’s Manual of Determinative Bacteriology, 9th.527–558 Baltimore: Williams & Wilkins;
    [Google Scholar]
  19. Jones K. F., Fischetti V. A. 1987; Biological and immuno-chemical identity of M protein on group G streptococci with M protein on group A streptococci. Infect Immun 55:502–506
    [Google Scholar]
  20. Jonsson H., Muller H. P. 1994; The type III Fc receptor from Streptococc us dysgalactiae is also an a2-macroglobulin receptor. Eur J Biochem 220:819–826
    [Google Scholar]
  21. Jonsson H., Frykberg E. R., Rantamaki L., Guss B. 1994; MAG, a novel plasma protein receptor from Streptococcus dysgalactiae . Gene 143:85–89
    [Google Scholar]
  22. Kilpper-Balz R., Schleifer K.-H. 1984; Nucleic acid hybridization and cell wall composition studies of pyogenic streptococci. FEMS Microbiol Lett 24:355–356
    [Google Scholar]
  23. Lancefield R. C. 1933; A serological differentiation of human and other groups of hemolytic streptococci. J Exp Med 57:571–595
    [Google Scholar]
  24. Lancefield R. C., Hare R. 1935; The serological differentiation of pathogenic and non-pathogenic strains of hemolytic streptococci from parturient women. J Exp Med 61:335–349
    [Google Scholar]
  25. Lawrence J., Yajko D. M., Hadley W. K. 1985; Incidence and characterization of /Miemolytic Streptococcus milleri and differentiation form S.pyogenes (group A), S.equisimilis (group C) and large-colony group G streptococci. J Clin Microbiol 22:772–777
    [Google Scholar]
  26. Momen H., Salles C. A. 1985; Enzyme markers for Vibrio cholerae identification of classical, El Tor and environmental strains. Trans R Soc Trop Med Hyg 79:773–776
    [Google Scholar]
  27. Musser J. M., Hewlett E. S., Peppier M. S., Selander R. K. 1986; Genetic diversity and relationships in populations of Bordetella spp. J Bacteriol 166:230–237
    [Google Scholar]
  28. Pasteur N., Pasteur G., Bonhomme F., Catala J., Britton-Davidian J. 1988; Laboratory techniques. In Practical Isozyme Genetics83–150 Chichester: Ellis Horwood;
    [Google Scholar]
  29. Selander R. K., Caugant D. A., Ochaman H., Musser J. M., Gilmour M. N., Whittan T. S. 1986; Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol 51:873–884
    [Google Scholar]
  30. Sippel K., Lämmler C. 1995; Further studies on immunoglobulin G and albumin-binding properties of streptococci of serological group L. J Vet Med B 42:421–426
    [Google Scholar]
  31. Sippel K., Dulffer-Schneitzer B., Lammler C. 1995; Characteristics properties of streptococci of serological group L. J Vet Med B 42:42–50
    [Google Scholar]
  32. Sjorbring U., Bjorck L., Kastern W. 1991; Streptococcal protein G: gene structure and binding properties. J Biol Chem 266:399–405
    [Google Scholar]
  33. Slifkin M., Gil G. M. 1983; Rapid chemical tests for the identification of groups A, B, C, F and G streptococci from throat cultures. J Clin Microbiol 18:29–32
    [Google Scholar]
  34. Sneath P. H. A., Sokal R. R. 1973 Numerical Taxonomy131–133 San Francisco: Freeman;
    [Google Scholar]
  35. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849
    [Google Scholar]
  36. Teixeira L. M., Facklam R. R., Steigerwalt A. G., Pigott N. E., Merquior V. L., Brenner D. J. 1994; Correlation between phenotypic characteristics and DNA relatedness within Enter-ococcus faecium strains. J Clin Microbiol 33:1520–1523
    [Google Scholar]
  37. Turner J. C., Hayden F. G., Lobo M. C., Ramirez C. E., Murren D. 1997; Epidemiology evidence for Lancefield group C beta-hemolytic streptococci as a cause of exudative pharyngitis in college students. J Clin Microbiol 35:1–4
    [Google Scholar]
  38. Vandamme P., Pot B., Falsen E., Kersters K., Devriese L. A. 1996; Taxonomic study of Lancefield streptococcal groups C, G, and L (Streptococcus dysgalactiae) and proposal of S.dysgalactiae subsp. equisimilis subsp. nov. Int J Syst Bacteriol 46:774–781
    [Google Scholar]
  39. Vieira V. V., Castro A. C. D. 1994; Biochemical properties and whole-cell protein profiles of group G streptococci isolated from dogs. J Appl Bacteriol 77:408–411
    [Google Scholar]
  40. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–164
    [Google Scholar]
  41. Whiley R. A., Fraser H., Hardie J. M., Beighton D. 1990; Phenotypical differentiation of Streptococcus intermedius.Streptococcus constellatus and Streptococcus anginosus strains within the ‘Streptococcus milleri group’. J Clin Microbiol 28:1497–1501
    [Google Scholar]
  42. Zahner V., Momen H., Salles C. A., Rabinovitch L. 1989; A comparative study of enzyme variation in Bacillus cereus and Bacillus thuringiensis . J Appl Bacteriol 67:275–282
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-48-4-1231
Loading
/content/journal/ijsem/10.1099/00207713-48-4-1231
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error