1887

Abstract

A Gram-positive, aerobic, spore-forming actinomycete strain, HR1-2, was isolated from a peat bog near Gifhorn, Lower Saxony, Germany. Comparative analysis of the 16S rDNA sequence indicated that HR1-2was phylogenetically related to members of the family , branching adjacent to and members of the genus . The affiliation to the family was supported by the presence of family-specific 16S rDNA signature nucleotides, DNA G+C content of 70 mol%, peptidoglycan of type A1′ (directly crossed-linked, presence of glycine, alanine, glutamic acid and -diaminopimelic acid in the peptide side-chain), menaquinone MK-9(H) as the major respiratory lipoquinone, polar lipid composition PII (phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylserine and phosphatidylinositolmannosides) and a glycolyl type of muramic acid. It differed from genera of the family by the lack of arabinose in whole-cell sugars and a unique nucleotide signature stretch between positions 1132 and 1143 ( numbering), 5’ CAAUUCGGUUG 3’. Morphologically strain HR1-2 resembles species but can be distinguished from them by the lack of arabinose in whole-cell sugars, the presence of 10-methyl C fatty acids and a distinct 16S rDNA sequence. Based on the unique combination of morphological, chemotaxonomic and phylogenetic properties a new genus, gen. nov., is proposed. The type species of this genus is sp. nov., and the type strain of is strain HR1-2(= DSM 44337).

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1998-10-01
2022-12-01
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