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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 48, Issue 3

sp. nov., from a North Sea oil-production well Free

Abstract

Rod-shaped, thermophilic bacteria with a sheath-like outer structure (‘toga’) were isolated from hot oilfield water of a North Sea oil reservoir. One of the isolates, designated SJ95, is an obligately anaerobic, sheathed, Gram-negative, fermentative bacterium capable of reducing elemental sulfur to hydrogen sulfide and tolerating high salt concentrations. The optimum growth conditions for this isolate are 58-60°C and pH 6.5-7.0 with 3-4% NaCl and 0.7% MgSO.7HO in the medium. Vitamins are required for growth. Growth is stimulated by yeast extract. Cells of strain SJ95vary in size from 1-2 to 40-50 μm in length and are motile with a subpolar flagellation. Cells grown on xylan have xylanase activity, presumably associated with the toga, and glucose isomerase activity was detected in xylose-grown cells. The DNA G+C content is 31 and 34 mol%, determined by the thermal denaturation and HPLC methods, respectively. Phylogenetically, strain SJ95is most closely related to with a 97.7% similarity level between their 16S rDNA sequences. The DNA-DNA reassociation value between the two DNAs was 35.6%. On the basis of differences in genotypic, phenotypic and immunological characteristics, strain SJ95(= DSM 10674) is proposed as the type strain of a new species, . It can be readily distinguished from by its motility.

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Keyword(s): oilfield water , Petrotoga mobilis sp. nov. and thermophile
© Society for General Microbiology, 1998
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1998-07-01
2025-05-15
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Petrotoga mobilis sp. nov., from a North Sea oil-production well
Int J Syst Evol Microbiol 48, 1007 (1998); https://doi.org/10.1099/00207713-48-3-1007
/content/journal/ijsem/10.1099/00207713-48-3-1007
/content/journal/ijsem/10.1099/00207713-48-3-1007
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