1887

Abstract

A polyphasic taxonomic study of 15 bovine and human strains assigned to the catalase-negative, urease-positive campylobacter (CNUPC) group identified these bacteria as a novel, ureolytic biovar of for which we propose the name bv. paraureolyticus: suitable reference strains are LMG 11764 (human isolate) and LMG 17590 (=CCUG 37579, bovine isolate). The present study confirmed previous findings showing that the salient biochemical tests used to differentiate bv. sputorum from bv. bubulus are not reproducible; and that the absolute validity of source-specific biovars of the species is questionable. A correlation between the results of numerical analysis of protein profiles and the reaction of strains in certain enzyme tests was, however, noted. Therefore, it is proposed that the infrasubspecific (biovar) divisions of should be revised to include bv. sputorum for catalase-negative strains; bv. fecalis for catalase-positive strains; and bv. paraureolyticus for urease-positive strains. Strains classified previously as bv. bubulus should be reclassified as bv. sputorum. The species description of is revised accordingly.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-48-1-195
1998-01-01
2023-01-31
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/48/1/ijs-48-1-195.html?itemId=/content/journal/ijsem/10.1099/00207713-48-1-195&mimeType=html&fmt=ahah

References

  1. Atabay H. I., Corry J. E. L., On S. L. W. 1997; Isolation and characterization of a novel catalase-negative, urease-positive Campylobacter from cattle faeces. Lett Appl Microbiol 24:59–64
    [Google Scholar]
  2. Atabay H. I., Corry J. E. L., Post D. E. 1996; Comparison of the productivity of a variety of selective media for Campylobacter and Arcobacter species. In Campylobacter VIII. Proceedings of the 8th International Workshop on Campylobacter, Helicobacter, and Related Organisms pp. 19–23 Edited by Newell D. G., Ketley J., Feldman R. A. New York: Plenum;
    [Google Scholar]
  3. Bastyns K., Chapelle S., Vandamme P., Goossens H., De Wachter R. 1994; Species-specific detection of campylobacters important in veterinary medicine by PCR amplification of 23S rDNA areas. Syst Appl Microbiol 17:563–568
    [Google Scholar]
  4. Borczyk A., Lior H., McKeown A., Svendsen H. 1988; Isolations of Campylobacter sputorum associated with human infections. In Campylobacter IV. Proceedings of the Fourth International Workshop on Campylobacter Infections, Goteborg, Sweden pp. 166–167 Edited by Kaijser B., Falsen E. Sweden: University of Goteborg;
    [Google Scholar]
  5. Charlier G., Dekeyser P., Florent A., Strobbe R., De Ley J. 1974; DNA base composition and biochemical characters of Campylobacter strains. Antonie Leeuwenhoek 40:145–151
    [Google Scholar]
  6. Corry J. E. L., Post D. E., Colin P., Laisney M. J. 1995; Culture media for the isolation of campylobacters. Int J Food Microbiol 26:43–76
    [Google Scholar]
  7. Costas M., Owen R. J., Jackman P. J. H. 1987; Classification of Campylobacter sputorum and allied campylobacters based on numerical analysis of electrophoretic protein patterns. Syst Appl Microbiol 9:125–131
    [Google Scholar]
  8. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142
    [Google Scholar]
  9. Firehammer B. D. 1965; The isolation of vibrios from ovine feces. Cornell Vet 55:482–494
    [Google Scholar]
  10. Genetics Computer Group 1994; Program manual for the Wisconsin package, version 8, August 1994. Genetics Computer Group; Madison, Wisconsin, USA:
    [Google Scholar]
  11. Goodwin C. S., Armstrong J. A., Chilvers T., Peters M., Collins M., D„ Sly L., McConnell W., Harper W. E. S. 1989; Transfer of Campylobacter pylori and Campylobacter mustelae to Helicobacter gen. nov. as Helicobacter pylori comb. nov. and Helicobacter mustelae comb. nov. respectively. Int J Syst Bacteriol 39:397–405
    [Google Scholar]
  12. Griffiths P. L., Park R. W. A. 1990; Campylobacters associated with human diarrhoeal disease. J Appl Bacteriol 69:281–301
    [Google Scholar]
  13. Harvey S. M., Greenwood J. R. 1983; Relationships among catalase-positive campylobacters determined by deoxyribonucleic acid-deoxyribonucleic acid hybridization. Int J Syst Bacteriol 33:275–284
    [Google Scholar]
  14. Holmes B., On S. L. W., Ganner M., Costas M. 1992; Some new applications of probabilistic identification. In Proceedings of the 1992 Conference on Taxonomy and Automated Identification of Bacteria, Prague, Czechoslovakia pp. 6–9 Edited by Schindler J. Prague: Czechoslovak Society for Microbiology;
    [Google Scholar]
  15. Laanbroek H. J., Kingma W., Veldkamp H. 1977; Isolation of an aspartate-fermenting, free-living Campylobacter species. FEMS Microbiol Lett 1:99–102
    [Google Scholar]
  16. Lastovica A., Le Roux E., Warren R., Klump H. 1993; Clinical isolates of Campylobacter mucosalis. J Clin Microbiol 31:2835–2836
    [Google Scholar]
  17. Lawson G. H. K., Leaver J. L., Pettigrew G. W., Rowland A. C. 1981; Some features of Campylobacter sputorum subsp. mucosalis subsp. nov., nom. rev. and their taxonomic significance. Int J Syst Bacteriol 31:385–391
    [Google Scholar]
  18. Lindblom G.-B., Sjdgren E., Hansson-Westerberg J., Kaijser B. 1995; Campylobacter upsaliensis, C. sputorum sputorum and C. concisus as common causes of diarrhoea in Swedish children. Scand J Infect Dis 27:187–188
    [Google Scholar]
  19. Loesche W. J., Gibbons R. J., Socransky S. S. 1965; Biochemical characteristics of Vibrio sputorum and relationship to Vibrio bubulus and Vibrio fetus. J Bacteriol 89:1109–1116
    [Google Scholar]
  20. On S. L. W. 1996; Identification methods for campylobacters, helicobacters, and related organisms. Clin Microbiol Rev 9:405–422
    [Google Scholar]
  21. On S. L. W., Bloch B., Holmes B., Hoste B., Vandamme P. 1995; Campylobacter hyointestinalis subsp. lawsonii subsp. nov., isolated from the porcine stomach, and an emended description of Campylobacter hyointestinalis. Int J Syst Bacteriol 45:767–774
    [Google Scholar]
  22. On S. L. W., Costas M., Holmes B. 1994; Classification and identification of Campylobacter sputorum using numerical analyses of phenotypic tests and of whole-cell protein profiles. Syst Appl Microbiol 17:543–553
    [Google Scholar]
  23. On S. L. W., Holmes B. 1991; Effect of inoculum size on the phenotypic characterisation of Campylobacter species. J Clin Microbiol 29:923–926
    [Google Scholar]
  24. On S. L. W., Holmes B. 1991; Reproducibility of tolerance tests that are useful in the identification of campylobacteria. J Clin Microbiol 29:1785–1788
    [Google Scholar]
  25. On S. L. W., Holmes B. 1992; Assessment of enzyme detection tests useful in identification of campylobacteria. J Clin Microbiol 30:746–749
    [Google Scholar]
  26. On S. L. W., Holmes B. 1995; Classification and identification of campylobacters, helicobacters and allied taxa by numerical analysis of phenotypic tests. Syst Appl Microbiol 18:374–390
    [Google Scholar]
  27. On S., L W., Holmes B., Sackin M. 1996; A probability matrix for the identification of campylobacters, helicobacters, and allied taxa. J Appl Bacteriol 81:425–432
    [Google Scholar]
  28. On S. L. W., Ridgwell F., Cryan B., Azadian B. S. 1992; Isolation of Campylobacter sputorum biovar sputorum from an axillary abscess. J Infect 24:175–179
    [Google Scholar]
  29. On S. L. W., Vandamme P. 1997; Identification and epidemiological typing of Campylobacter hyointestinalis subspecies by phenotypic and genotypic methods and description of novel subgroups. Syst Appl Microbiol 20:238–247
    [Google Scholar]
  30. Pearson W. R. 1990; Rapid and sensitive sequence comparison with fastp and fasta . Methods Enzymol 183:63–98
    [Google Scholar]
  31. Pot B., Vandamme P., Kersters K. 1994; Analysis of electrophoretic whole-organism protein fingerprints. In Modern Microbial Methods (Chemical Methods in Prokaryotic Systematics series), pp. 493–521 Edited by Goodfellow M., O’Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  32. Prevot A. R. 1940; Etudes de systematique bacterienne. V. Essai de classification des vibrions anaerobies. Ann Inst Pasteur 64:117–125
    [Google Scholar]
  33. Roop R. M., II, Smibert R. M., Johnson J. L., Krieg N. R. 1985; DNA homology studies of the catalase-negative campylobacters and ‘ Campylobacter fecalis ’, an emended description of Campylobacter sputorum and proposal of the neotype strain of Campylobacter sputorum. Can J Microbiol 31:823–831
    [Google Scholar]
  34. Roop R. M., II, Smibert R. M., Johnson J. L., Krieg N. R. 1985; Campylobacter mucosalis (Lawson, Leaver, Pettigrew and Rowland 1981) comb. nov.: emended description. Int J Syst Bacteriol 35:189–192
    [Google Scholar]
  35. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd. edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  36. Sebald M., Véron M. 1963; Teneur en bases de TADN et classification des vibrions. Ann Inst Pasteur 105:897–910
    [Google Scholar]
  37. Skirrow M. B. 1994; Diseases due to Campylobacter, Helicobacter and related bacteria. J Comp Pathol 111:113–149
    [Google Scholar]
  38. Tunnidiff R. 1914; An anaerobic vibrio isolated from a case of acute bronchitis. J Infect Dis 15:350–351
    [Google Scholar]
  39. Ursing J., Firehammer B. D. 1985; Genetic relationship between ‘ Campylobacter fecalis ’ and Campylobacter sputorum subsp bubulus. Acta Pathol Microbiol Immunol Scand Sect B 93:377–378
    [Google Scholar]
  40. Van Camp G., De Peer Y. V., Nicolai S., Neefs J.-M., Vandamme P., De Wachter R. 1993; Structure of 16S and 23S ribosomal RNA genes in Campylobacter species: phylogenetic analysis of the genus Campylobacter and presence of internal transcribed spacers. Syst Appl Microbiol 16:361–368
    [Google Scholar]
  41. Vandamme P., Danashvar M. I., Dewhirst F. E., Paster B. J., Kersters K., Goossens H., Moss C. W. 1995; Chemo- taxonomic analyses of Bacteroides gracilis and Bacteroides ureolyticus and reclassification of B. gracilis as Campylobacter gracilis comb. nov. Int J Syst Bacteriol 45:145–152
    [Google Scholar]
  42. Vandamme P., De Ley J. 1991; Proposal for a new family, Campylobacteraceae. Int J Syst Bacteriol 41:451–455
    [Google Scholar]
  43. Vandamme P., Falsen E., Rossau R., Hoste B., Segers P., Tytgat R., De Ley J. 1991; Revision of Campylobacter, Helicobacter and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. Int J Syst Bacteriol 41:88–103
    [Google Scholar]
  44. Vandamme P., Vancanneyt M., Pot B., Mels L., Hoste B., Dewettinck D., Vlaes L., Van Den Borre C., Higgins R., Kersters K., Butzler J.-P., Goossens H. 1992; Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aero tolerant bacterium isolated from veterinary specimens. Int J Syst Bacteriol 42:344–356
    [Google Scholar]
  45. Vandamme P., Van Doom L.-J., Al Rashid S. T., Quint W. G. V., Chan V., L, Van der Plads J., On S. L. W. 1997; Campylobacter hyoilei Alderton et al. 1995 and Campylobacter coli Veron and Chatelain 1973 are subjective synonyms. Int J Syst Bacteriol 47:1055–1060
    [Google Scholar]
  46. Véron M., Chatelain R. 1973; Taxonomic study of the genus Campylobacter Sebald and Veron and designation of the neotype strain for the type species, Campylobacter fetus (Smith and Taylor) Sebald and Veron. Int J Syst Bacteriol 23:122–134
    [Google Scholar]
  47. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-48-1-195
Loading
/content/journal/ijsem/10.1099/00207713-48-1-195
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error