1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 47, Issue 4

Classification of Austrian Rhizobia and the Mexican Isolate FL27 Obtained from L. as Free

Abstract

The phylogenetic positions of four rhizobial strains obtained from nodules of common bean plants ( L.) grown in an Austrian soil and of the Mexican bean isolate FL27 are described. Analysis of the 16S rRNA genes revealed sequences almost identical to that of the type strain, R602sp, with a maximum of two nucleotide substitutions. Comparison of the 16S rRNA gene sequences with those from other bacteria indicated highest similarity to sp. strain OK-50, IAM 12609, and DNA homology determined by DNA-DNA hybridization was high among the Austrian isolates and R602sp (45 to 90%) and ranged from 21 to 65% with FL27, but hybridization analysis revealed very low homology to the recognized common bean-nodulating species, bv. phaseoli, , and Ribosomal gene organization was studied by Southern hybridization with the 16S rRNA gene and temperature gradient gel electrophoresis, indicating identical organizations and the presence of three identical 16S rRNA copies in the genome of this species. The six strains investigated showed different plasmid profiles based on their geographical origins. We propose that the Austrian isolates and the Mexican strain FL27 are members of the species

  • Published Online:
© Society for General Microbiology, 1997
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-47-4-1097
1997-10-01
2024-05-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/47/4/ijs-47-4-1097.html?itemId=/content/journal/ijsem/10.1099/00207713-47-4-1097&mimeType=html&fmt=ahah

References

  1. Amarger N., Macheret V., Laguerre G. 1997; Rhizobium gallicum sp. nov. and Rhizobium giardinii sp. nov., from Phaseolus vulgaris nodules. Int. J. Syst. Bacteriol. 47:996–1006
     [Google Scholar]
  2. Amarger N., Bours M., Revoy F., Allard M.-R., Laguerre G. 1994; R. tropici nodulates field-grown Phaseolus vulgaris in France. Plant Soil 161:147–156
     [Google Scholar]
  3. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K.ed 1995 Current protocols in molecular biology. Wiley; New York, N.Y.:
     [Google Scholar]
  4. Brom S., García de los Santos A., de Lourdes Girard M., Davila G., Palacios R., Romero D. 1991; High-frequency rearrangements in Rhizobium leguminosarum bv. phaseoli plasmids. J. Bacterio] 173:1344–1346
     [Google Scholar]
  5. Eardly B. D., Young J. P. W., Selander R. K. 1992; Phylogenetic position of Rhizobium sp. strain Or 191, a symbiont of both Medicago sativa and Phaseolus vulgaris, based on partial sequences of the 16S rRNA and nifH genes. Appl. Environ. Microbiol. 58:1809–1815
     [Google Scholar]
  6. Eardly B. D., Wang F.-S., Whittam T. S., Selander R. K. 1995; Species limits in Rhizobium populations that nodulate the common bean (Phaseolus vulgaris). Appl. Environ. Microbiol. 61:507–512
     [Google Scholar]
  7. Gebts P. 1990; Biochemical evidence bearing on the domestication of Phaseolus (Fabaceae) beans. Econ. Bot. 44:28–38
     [Google Scholar]
  8. Graham P. H., Sadowsky M. J., Keyser H. H., Barnet Y. M., Bradley R. S., Cooper J. E., de Ley D. J., Jarvis B. D. W., Roslycky E. B., Strijdom B. W., Young J. P. W. 1991; Proposed minimal standards for the description of new genera and species of root- and stem-nodulating bacteria. Int. J. Syst. Bacteriol. 41:582–587
     [Google Scholar]
  9. Hernandez-Lucas I., Segovia L., Martinez-Romero E., Pueppke S. G. 1995; Phylogenetic relationships and host range of Rhizobium spp. that nodulate Phaseolus vulgaris L. Appl. Environ. Microbiol. 61:2775–2779
     [Google Scholar]
  10. Higgins D. G., Bleasby A. J., Fuchs R. 1992; CLUSTAL V: improved software for multiple sequence alignment. Comput. Appl. Biosci. 8:189–191
     [Google Scholar]
  11. Huber L, Selenska-Pobell S. 1994; Pulsed-field gel electrophoresisfingerprinting, genome size estimation and rrn loci number of Rhizobium galegae. J. Appl. Bacteriol. 77:528–533
     [Google Scholar]
  12. Hynes M. F., Simon R., Piihler A. 1985; The development of plasmidfree strains of Agrobacterium tumefaciens by using incompatibility with a Rhizobium meliloti plasmid to eliminate pAtC58. Plasmid 13:99–105
     [Google Scholar]
  13. Jordan D. C. 1984 Family III. Rhizobiaceae. 235–242 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 Williams & Wilkins; Baltimore, Md.:
     [Google Scholar]
  14. Jukes T. H., Cantor C. R. 1969 Evolution of protein molecules. 21–132 Munro H. N.ed Mammalian protein metabolism Academic Press; New York, N.Y.:
     [Google Scholar]
  15. Klijn N., Bovie C., Dommes J., Hoolwerf J. D., van der Waals C. B., Weerkamp A. H., Nieuwenhof F. F. J. 1994; Identification of Clostridium tyrobutyricum and related species using sugar fermentation, organic acid formation and DNA probes based on specific 16S rRNA sequences. Syst. Appl. Microbiol. 17:249–256
     [Google Scholar]
  16. Laguerre G., Fernandez M. P., Edel V., Normand P., Amarger N. 1993; Genomic heterogeneity among French Rhizobium strains isolated from Phaseolus vulgaris L. Int. J. Syst. Bacteriol. 43:761–767
     [Google Scholar]
  17. Martínez E., Pardo M. A., Palacios R., Cevallos M. A. 1985; Reiteration of nitrogen fixation gene sequences and specificity to Rhizobium in nodulation and nitrogen fixation in Phaseolus vulgaris. J. Gen. Microbiol. 131:1779–1786
     [Google Scholar]
  18. Martinez-Murcia A. J., Benlloch S., Collins M. D. 1992; Phylogenetic interrelationships of members of the genera Aeromonas and Plesiomonas as determined by 16S ribosomal DNA sequencing: lack of congruence with results of DNA-DNA hybridizations. Int. J. Syst. Bacteriol. 42:412–421
     [Google Scholar]
  19. Martinez-Romero E. 1994; Recent developments in Rhizobium taxonomy. Plant Soil 161:11–20
     [Google Scholar]
  20. Martinez-Romero E., Segovia L., Mercante F. M., Franco A. A., Graham P., Pardo M. A. 1991; Rhizobium tropici, a novel species nodulating Phaseolus vulgaris L. beans and Leucaena sp. trees. Int. J. Syst. Bacteriol. 41:417–426
     [Google Scholar]
  21. Miller J. H. 1972 Experiments in gene fusions. Cold Spring Harbor Laboratory; Cold Spring Harbor, N.Y.:
     [Google Scholar]
  22. Noel K. D., Sánchez F., Fernández L., Leemans J., Cevallos M. A. 1984; Rhizobium phaseoli symbiotic mutants with transposon Tn5 insertions. J. Bacteriol. 158:148–155
     [Google Scholar]
  23. Ndlling J., Hahn D., Ludwig W., de Vos W. M. 1993; Phylogenetic analysis of thermophilic Methanobacterium sp.: evidence for a common formate-utilizing ancestor. Syst. Appl. Microbiol. 16:208–215
     [Google Scholar]
  24. Nübel U., Engelen B., Felske A., Snaidr J., Wieshuber A., Amann R. I., Ludwig W., Backhaus H. 1996; Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis. J. Bacteriol. 178:5636–5643
     [Google Scholar]
  25. Oyaizu H., Naruhashi N., Gamou T. 1992; Molecular methods of analysing bacterial diversity: the case of rhizobia. Biodivers. Conserv. 1:237–249
     [Google Scholar]
  26. Oyaizu H., Matsumoto S., Minamisawa K., Gamou T. 1993; Distribution of rhizobia in leguminous plants surveyed by phylogenetic identification. J. Gen. Appl. Microbiol. 39:339–354
     [Google Scholar]
  27. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA 82:2444–2448
     [Google Scholar]
  28. Piñero D., Martinez E., Selander R. K. 1988; Genetic diversity and relationships among isolates of Rhizobium leguminosarum biovar phaseoli. Appl. Environ. Microbiol. 54:2825–2832
     [Google Scholar]
  29. Prakash R. K., Atherly A. G. 1986; Plasmids of Rhizobium and their role in symbiotic nitrogen fixation. Int. Rev. Cytol. 104:1–24
     [Google Scholar]
  30. Quinto C., de la Vega H., Flores M., Fernandez I., Ballado T., Soberon G., Palacios R. 1982; Reiteration of nitrogen fixation gene sequences in Rhizobium phaseoli. Nature (London) 229:724–726
     [Google Scholar]
  31. Rao J. R., Fenton M., Jarvis B. D. W. 1994; Symbiotic plasmid transfer in Rhizobium leguminosarum biovar trifolii and competition between the inoculant strain ICMP2163 and transconjugant soil bacteria. Soil Biol. Biochem. 26:339–351
     [Google Scholar]
  32. Roberts G. P., Leps W. T., Silver L. E., Brill W. J. 1980; Use of two-dimensional polyacrylamide gel electrophoresis to identify and classify Rhizobium strains. Appl. Environ. Microbiol. 39:414–422
     [Google Scholar]
  33. Romero D., Brom S., Martinez-Salazar J., de Lourdes Girard M., Palacios R., Davila G. 1991; Amplification and deletion of a nod-nif region in the symbiotic plasmid of Rhizobium phaseoli. J. Bacteriol. 173:2435–2441
     [Google Scholar]
  34. Saitou R., Nei M. 1987; A neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 44:406–425
     [Google Scholar]
  35. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463–5467
     [Google Scholar]
  36. Schofield P. R., Gibson A. H., Dudman W. F., Watson J. M. 1987; Evidence for genetic exchange and recombination of Rhizobium symbiotic plasmids in a soil population. Appl. Environ. Microbiol. 53:2942–2947
     [Google Scholar]
  37. Scholia M. H., Moorefield J. A., Elkan G. H. 1990; DNA homology between species of the rhizobia. Syst. Appl. Microbiol. 13:288–294
     [Google Scholar]
  38. Segovia L., Young J. P. W., Martinez-Romero E. 1993; Reclassification of American Rhizobium leguminosarum biovar phaseoli type I strains as Rhizobium etli sp. nov. Int. J. Syst. Bacteriol. 43:374–377
     [Google Scholar]
  39. Sessitsch A., Jjeinba P. K., Hardarson G., Akkermans A. D. L., Wilson K. J. 1997; Measurement of the competitiveness index of Rhizobium tropici strain CIAT 899 derivatives marked with the gusA gene. Soil Biol. Biochem. 29:1099–1110
     [Google Scholar]
  40. Sessitsch A., Hardarson G., de Vos W. M., Wilson K. J. Use of marker genes in competition studies of Rhizobium. Submitted for publication
  41. Sessitsch A., Hardarson G., Akkermans A. D. L., de Vos W. M. 1997; Characterization of Rhizobium etli and other Rhizobium spp. that nodulate Phaseolus vulgaris L. in an Austrian soil. Mol. Ecol. 6:601–608
     [Google Scholar]
  42. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol. 44:846–849
     [Google Scholar]
  43. Swofford D. L. 1993 PAUP: phylogenetic analysis using parsimony. Version 3.1. The Illinois Natural History Survey; Champaign, Ill:
     [Google Scholar]
  44. van Berkum P., Beyene D., Eardly B. D. 1996; Phylogenetic relationships among Rhizobium species nodulating the common bean (Phaseolus vulgaris L.). Int. J. Syst. Bacteriol. 46:240–244
     [Google Scholar]
  45. van de Peer Y., de Wachter R. 1994; TREECON for Windows: a software package for the construction and drawing of evolutionary trees for Microsoft Windows environment. Comput. Appl. Biosci. 10:569–570
     [Google Scholar]
  46. Vincent J. M. 1970 A manual for the practical study of root nodule bacteria. 73–97 International biological programme handbook Blackwell Scientific Publications, Ltd.; Oxford, United Kingdom.:
     [Google Scholar]
  47. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 43:374–377
     [Google Scholar]
  48. Wilson K. J., Sessitsch A., Corbo J. C., Giller K. E., Akkermans A. D. L., Jefferson R. A. 1995; p-Glucuronidase (GUS) transposons for ecological and genetic studies of rhizobia and other Gram-negative bacteria. Microbiology 141:1691–1705
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-47-4-1097
Loading
Classification of Austrian Rhizobia and the Mexican Isolate FL27 Obtained from Phaseolus vulgaris L. as Rhizobium gallicum
Int J Syst Evol Microbiol 47, 1097 (1997); https://doi.org/10.1099/00207713-47-4-1097
/content/journal/ijsem/10.1099/00207713-47-4-1097
/content/journal/ijsem/10.1099/00207713-47-4-1097
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error