1887

Abstract

The intron-encoded enzyme I-I provides an excellent tool for rapidly examining the organization of genomes of related species of bacteria. strains belonging to serovars O1 and O139 have 9 I-I sites in their genomes, and . strains belonging to serovars non-Ol and non-O139 have 10 I-I sites in their genomes. This information can be used as a criterion to differentiate O1 strains from non-O1 and non-O139 strains. To our knowledge, intraspecies variation in the number of operons has not been reported in any other organism. Our data revealed extensive restriction fragment length polymorphism based on a comparison of the I-I digestion profiles of strains belonging to different serovars and biovars. From the analysis of partial digestion products, I-I macrorestriction maps of several classical, E1 Tor, and O139 strains were constructed. While the linkage maps are conserved within biovars, linkage maps vary substantially between biovars.

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1997-07-01
2023-02-01
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