16S/23S intergenic spacer regions from the rRNA operons of two strains of “ Liberobacter asiaticum” and one strain of “ Liberobacter africanum” were cloned and sequenced. The intergenic spacers of the two “ L. asiaticum” strains studied are identical and contain the genes for isoleucine tRNA (tRNA) and alanine tRNA (tRNA) separated by 11 nucleotides. The intergenic spacer of the “ L. africanum” strain contains only one tRNA gene (tRNA). The level of homology between the intergenic spacers of the two liberobacter species is 79.46%. Ribosomal operons with 16S/23S spacer regions other than those studied might be present in the two “ Liberobacter” species.


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