. a mild plant pathogen, , and EF group 1 strains (clustered by an immunological method) were investigated by a polyphasic approach with DNA-rRNA and DNA-DNA hybridizations and auxanography on 147 substrates. Our results show that they all belong to the genus . In addition to , two other species are described: and a new unnamed species, species 3, containing mainly strains of clinical origin. The three species can be differentiated on the basis of their auxanographic features and DNA-DNA similarities. The type strain of is NCPPB 1027 (=LMG 2286): Representative strains of the third species are strains CCUG 189 (=LMG 5523), CCUG 10263 (=LMG 5934), and CCUG 11060 (=LMG 5321). It has been confirmed that is an endophytic diazotroph. It colonizes the roots, the stems, and predominantly the leaves of sugarcane ( spp.), while colonizes in large numbers many different species of the Gramineae. Both diazotrophic species could be differentiated with -erythritol and -acetylglucosamine. Oligonucleotide probes based on partial sequences of the 23S rRNA of and (HS and HR probes, respectively), were constructed and used as diagnostic probes.


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