1887

Abstract

By using selective enrichment of polyhydroxyalkanoate-degrading bacteria and poly(3-hydroxyvalerate)-containing granules from as the carbon source, 10 new strains were isolated; these strains were able to degrade poly(3-hydroxyvalerate), as well as poly(3-hydroxybutyrate), in vitro. The new isolates were characterized and identified by comparing them with LMG 2207 (T = type strain). Like LMG 2207 cells, the cells of the 10 new isolates contained mainly hexadecenoic, hexadecanoic, octadecenoic, and dodecanoic acids, as well as hydroxylated fatty acids, and exhibited respiration in the presence of methylpyruvate, 3-hydroxybutyrate, and 4-hydroxybutyrate, but not in the presence of the 92 other carbon sources included in Biolog GN microplates. The protein patterns of the new isolates were almost identical to each other and very similar to the protein pattern of LMG 2207. Some of the new isolates, but not LMG 2207, contained megaplasmids that were about 200 kbp long. The 16S ribosomal DNA genes of strain A62, a representative of the 10 new isolates, and of LMG 2207 exhibited more than 0.99 sequence similarity. The DNA-DNA reassociation value for two representative strains was 100%, and the levels of DNA-DNA reassociation between these strains and the type strain were 60 and 61%. The taxonomy of is briefly discussed.

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1996-07-01
2024-11-04
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References

  1. Alcorn S. M., Oram T. V., Steigerwalt A. G., Foster J. M., Fogleman J. C., Brenner D. J. 1991; Taxonomy and pathogenicity of Erwinia cacticida sp. nov. Int. J. Syst. Bacteriol 41:197–212
    [Google Scholar]
  2. Anderson A. J., Dawes E. A. 1990; Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxy alkanoates. Microbiol. Rev 54:450–472
    [Google Scholar]
  3. Anderson A. J., Haywood G. W., Dawes E. A. 1990; The biosynthesis and composition of bacterial polyhydroxyalkanoates. Int. J. Biol. Macromol 12:102–105
    [Google Scholar]
  4. Brandl H., Gross R. A., Lenz R. W., Fuller R. C. 1988; Pseudomonas oleovorans as a source of poly(p-hydroxyalkanoates) for potential applications as biodegradable polyesters. Appl. Environ. Microbiol 54:1977–1982
    [Google Scholar]
  5. Briese B. H., Jendrossek D., Schlegel H. G. 1994; Degradation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by aerobic sewage sludge. FEMS Microbiol. Lett 117:107–112
    [Google Scholar]
  6. Brosius J., Dull T. J., Sleeter D. D., Noller H. F. 1981; Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J. Mol. Biol 148:107–127
    [Google Scholar]
  7. Delafield F. P., Doudoroff M., Palleroni N. J., Lusty J., Contopoulos R. 1965; Decomposition of poly-p-hydroxybutyrate by pseudomonads. J. Bacteriol 90:1455–1466
    [Google Scholar]
  8. De Ley J. 1970; Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J. Bacteriol 101:738–754
    [Google Scholar]
  9. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem 12:133–142
    [Google Scholar]
  10. De Vos P., De Ley J. 1983; Intraand intergeneric similarities of Pseudomonas and Xanthomonas ribosomal ribonucleic acid cistrons. Int. J. Syst. Bacteriol 33:487–509
    [Google Scholar]
  11. Doi Y. 1990 Microbial polyesters VCH Publishers, Inc.; New York:
    [Google Scholar]
  12. Gillis M., Tran Van V., Bardin R., Goor M., Hebbar P., Willems A., Segers P., Kersters K., Heulin T., Fernandez M. P. 1995; Polyphasic taxonomy in the genus Burkholderia leading to an emended description of the genus and proposition of Burkholderia vietnamiensis sp. nov. for N2-fixing isolates from rice in Vietnam. Int. J. Syst. Bacteriol 45:274–289
    [Google Scholar]
  13. Jendrossek D., Frisse A., Behrends A., Andermann M., Kratzin H. D., Stanislawski T., Schlegel H. G. 1995; Biochemical and molecular characterization of the Pseudomonas lemoignei polyhydroxyalkanoate depolymerase system. J. Bacteriol 177:596–607
    [Google Scholar]
  14. Jendrossek D., Knoke I., Habibian R. H., Steinbiichel A., Schlegel H. G. 1993; Degradation of poly(3-hydroxybutyrate), PHB, by bacteria and purification of novel PHB depolymerase of Comamonas sp. J. Environ. Polymer Degrad 1:53–63
    [Google Scholar]
  15. Jukes T. H., Cantor C. R. 1969 Evolution of protein molecules. 21–132 Munro H. N.ed Mammalian protein metabolism Academic Press; New York:
    [Google Scholar]
  16. Kado C. I., Liu S.-T. 1981; Rapid procedure for detection and isolation of large and small plasmids. J. Bacteriol 145:1365–1373
    [Google Scholar]
  17. Karlson U., Dwyer D. F., Hooper S. W., Moore E. R. B., Timmis K. N., Eltis L. D. 1993; Two independently regulated cytochromes P-450 in a Rhodococcus rhodochrous strain that degrades 2-ethoxyphenol and 4-methoxybenzoate. J. Bacteriol 175:1467–1474
    [Google Scholar]
  18. Kersters K., De Ley J. 1984 Genus Alcaligenes Castellani and Chalmers 1919, 936AL. 361–373 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 Williams and Wilkins; Baltimore:
    [Google Scholar]
  19. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685
    [Google Scholar]
  20. Larsen N., Olsen G. J., Maidak B. L., McCaughey M. J., Overbeek R., Macke T. J., Marsch T. L., Woese C. R. 1993; The Ribosomal Database Project. Nucleic Acids Res 21:3021–3023
    [Google Scholar]
  21. Li X., Dorsch M., Del Dot T., Sly L. L., Stackebrandt E., Hayward A. C. 1993; Phylogenetic studies of the rRNA group II pseudomonads based on 16S rRNA gene sequences. J. Appl. Bacteriol 74:324–329
    [Google Scholar]
  22. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol 3:208–218
    [Google Scholar]
  23. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol 5:109–118
    [Google Scholar]
  24. Mergaert J., Anderson C., Wouters A., Swings J. 1994; Microbial degradation of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-γ-3-hydroxyvalerate) in compost. J. Environ. Polymer Degrad 2:177–183
    [Google Scholar]
  25. Mergaert J., Anderson C., Wouters A., Swings J., Kersters K. 1992; Biodegradation of polyhydroxyalkanoates. FEMS Microbiol. Rev 103:317–322
    [Google Scholar]
  26. Mergaert J., Verdonck L., Kersters K. 1993; Transfer of Erwinia ananas (synonym, Erwinia uredovora) and Erwinia stewartii to the genus Pantoea emend, as Pantoea ananas (Serrano 1928) comb. nov. and Pantoea stewartii (Smith 1898) comb, nov., respectively, and description of Pantoea stewartii subsp. indologenes subsp. nov. Int. J. Syst. Bacteriol 43:162–173
    [Google Scholar]
  27. Mergaert J., Webb A., Anderson C., Wouters A., Swings J. 1993; Microbial degradation of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in soils. Appl. Environ. Microbiol 59:3233–3238
    [Google Scholar]
  28. Mergaert J., Wouters A., Anderson C., Swings J. 1995; In situ biodegradation of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-ci?-3-hydroxyvalerate) in natural waters. Can. J. Microbiol 41:Suppl. 1154–159
    [Google Scholar]
  29. Mergaert J., Wouters A., Swings J. 1994; Estimation of the intrinsic biodiversity among poly(3-hydroxyalkanoates) degrading streptomycetes using gas chromatographic analysis of fatty acids. Syst. Appl. Microbiol 17:601–612
    [Google Scholar]
  30. Muller B., Jendrossek D. 1993; Purification and properties of poly(3- hydroxyvaleric acid) depolymerase from Pseudomonas lemoignei. Appl. Microbiol. Biotechnol 38:487–492
    [Google Scholar]
  31. Olsen G. J. 1987; The earliest phylogenetic branchings: comparing rRNA- based evolutionary trees inferred with various techniques. Cold Spring Harbor Symp. Quant. Biol 52:825–838
    [Google Scholar]
  32. Olsen G. J., Larsen N., Woese C. R. 1991; The Ribosomal RNA Database Project. Nucleic Acids Res 19:2017–2021
    [Google Scholar]
  33. Schirmer A., Jendrossek D., Schlegel H. G. 1993; Degradation of poly(3-hydroxyoctanoic acid) [P(3HO)J by bacteria: purification and properties of a P(3HO) depolymerase from Pseudomonas fluorescens GK13. Appl. Environ. Microbiol 59:1220–1227
    [Google Scholar]
  34. Schinner A., Matz C., Jendrossek D. 1995; Substrate specificities of poly(hydroxyalkanoate)-degrading bacteria and active site studies on the extracellular poly(3-hydroxyoctanoic acid) depolymerase of Pseudomonas fluorescens GK13. Can. J. Microbiol 41:Suppl. 1170–179
    [Google Scholar]
  35. Schlegel H. G., Kaltwasser H., Gottschalk G. 1961; Ein Submersverfahren zur Kultur wasserstoffoxidierender Bakterien: Wachstumsphysiologische Untersuchungen. Arch. Mikrobiol 38:209–222
    [Google Scholar]
  36. Shin Y. K., Hiraishi A., Sugiyama J. 1993; Molecular systematics of the genus Zoogloea and emendation of the genus. Int. J. Syst. Bacteriol 43:826–831
    [Google Scholar]
  37. Sneath P. H. A., Sokal R. R. 1973 The principles and practice of numerical classification Freeman and Co.; San Francisco:
    [Google Scholar]
  38. Stackebrandt E., Murray R. G. E., Triiper H. G. 1988; Proteobacteria classis nov., a name for the phylogenetic taxon that includes the “purple bacteria and their relatives/* Int. J. Syst. Bacteriol 38:321–325
    [Google Scholar]
  39. Steinbiichel A. 1991 Polyhydroxyalkanoic acids. 1123–213 Byrom D.ed Biomaterials Macmillan Press; London:
    [Google Scholar]
  40. Stelnbiichel A., Debzi E., Marchessault R. H., Timm A. 1993; Synthesis and production of poly(3-hydroxyvaleric acid) homopolymer by Chromobacterium violaceum. Appl. Microbiol. Biotechnol 39:443–449
    [Google Scholar]
  41. Steinbiichel A., Valentin H. E. 1995; Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiol Lett 128:219–228
    [Google Scholar]
  42. Urakami T., Itro-Yoshida C., Araki H., Kijima T., Suzuki K.-I., Komagata K. 1994; Transfer of Pseudomonas plantarii and Pseudomonas glumae to Burkholderia as Burkholderia spp. and description of Burkholderia vandii sp. nov. Int. J. Syst. Bacteriol 44:235–245
    [Google Scholar]
  43. Wilson K. 1987 Preparation of genomic DNA from bacteria. 241–242 Ausubeland F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K.ed Current protocols in molecular biology John Wiley; New York:
    [Google Scholar]
  44. Yabuuchi E., Kosako Y., Oyaizu H., Yano I., Hotta H., Hashimoto Y., Ezaki T., Arakawa M. 1992; Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. Microbiol. Immunol 36:1251–1275
    [Google Scholar]
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