1887

Abstract

A taxonomic characterization was carried out on strains of the bacteria that cause the brown ring disease of clams. On the basis of their phenotypic and genotypic characteristics, these strains can be considered to constitute a new taxonomic unit, distinct from other species. The guanine-plus-cytosine content of the strains ranged between 42.9 and 45.5 mol% (43.2 mol% for the proposed type strain). DNA-DNA hybridization studies showed 100% intragroup relatedness, but levels of genetic relatedness to the reference strains of different species tested ranged between 15 and 58%. The strains have all the properties characteristic of the genus and can be clearly differentiated from other species of this genus by their growth at 4°C and their negative responses for growth at 30°C and in 6% NaCl, arginine dehydrolase, lysine decarboxylase, ornithine decarboxylase, and Voges-Proskauer reaction. The name is proposed for the new species; strain B1090 (CECT 4600) is the type strain.

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1996-04-01
2022-08-18
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References

  1. Balebona M. C. 1994; Caracterizacion y mecanismos de virulencia de cepas del genero Vibrio aisladas de patologias de doradas (Sparus aurata. L.) cultivadas. Ph.D. thesis University of Malaga; Malaga, Spain:
    [Google Scholar]
  2. Castro D. 1994; Patologias de origen bacteriano en almejas cultivadas Tapes decussatus y T. phillipinarum. Ph.D. thesis University of Malaga; Malaga, Spain:
    [Google Scholar]
  3. Castro D., Luque A., Martinez-Manzanares E., Borrego J. J. 1993 Determinacion de la flora microbiana prevalente en almejas japonesas (Tapesphilippinarum) sanas y afectadas por la enfermedad del anillo marron. 621–626 Cervino A., Landin A., de Coo A., Guerra A., Torres M.ed Aetas IV Congreso Nacional de Acuicultura Grafinova; Santiago de Compostela, Spain:
    [Google Scholar]
  4. Castro D., Martinez-Manzanares E., Luque A., Fouz B., Morinigo M. A., Borrego J. J., Toranzo A. E. 1992; Characterization of strains related to brown ring disease outbreaks in southwestern Spain. Dis. Aquat. Org 14:229–236
    [Google Scholar]
  5. Cowan S. T. 1974 Cowan and Steel’s manual for the identification of medical bacteria, 2nd. Cambridge University Press; London:
    [Google Scholar]
  6. de la Pena L. D., Tamaki T., Momoyama K., Nakai T., Muroga K. 1993; Characteristics of the causative bacterium of vibriosis in the kuruma prawn, Penaeus japonicus. Aquaculture 115:1–12
    [Google Scholar]
  7. De Ley J., Tijtgat R. 1970; Evaluation of membrane filter methods for DNA-DNA hybridization. Antonie van Leeuwenhoek J. Microbiol. Serol 36:461374
    [Google Scholar]
  8. Ferragut C., Leclerc H. 1976; Etude comparative des methodes de determination du Tm de FADN bacterien. Annal. Microbiol. (Paris) 127:223–235
    [Google Scholar]
  9. Fouz B., Larsen J. L., Nielsen B., Baija J. L., Toranzo A. E. 1992; Characterization of Vibrio damsela strains isolated from turbot Scophthalmus maximus in Spain. Dis. Aquat. Org 12:155–166
    [Google Scholar]
  10. Fujiwara M., Uyeno Y., Iwao A. 1993; A Vibrio sp. associated with mortalities in cockle larvae Fulvia mutica (Mollusca: Cardiidae). Gyobyo Kenkyu 28:83–89
    [Google Scholar]
  11. Hada H. S., West P. A., Lee J. V., Stemmier J., ColweJl R. R. 1984; Vibrio tubiashii sp. nov., a pathogen of bivalve mollusks. Int. J. Syst. Bacteriol 34:1–4
    [Google Scholar]
  12. Holt J. E., Krieg N. R., Sneath P. H. A., Staley J. T., Williams S. T. 1993 Bergey’s manual of determinative bacteriology. , 9th. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  13. Johnson J. L. 1981 Genetic characterization. 450–472 Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W, Wood W. A, Krieg N. R., Phillips G. B.ed Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  14. Lee J. V., Donovan T. J. 1985 Vibrio, Aeromonas and Plesiomonas,. 13–33 Collins C. H., Grange J. M.ed Isolation and identification of microorganisms of medical and veterinary importance Academic Press Inc. Ltd.; London:
    [Google Scholar]
  15. Lupiani B., Ledo A., Dopazo C. P., Baya A., Toranzo A. E., Baija J. L. 1989 Virulence of Vibrio splendidus and turbot reovirus (TRV) for three different fish species. 103 Fourth International Conference on Diseases of Fish and Shellfish European Association of Fish Pathologists; Santiago de Compostela, Spain:
    [Google Scholar]
  16. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol 5:208–218
    [Google Scholar]
  17. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol 5:109–118
    [Google Scholar]
  18. Orta M. M. 1994; Estudio de la microbiota autoctona en piscifactorias dedicadas al cultivo de dorada espanola (Sparus aurata), con especial atencion al patogeno oportunista Vibrio alginolyticus. Ph.D. thesis University of Seville; Seville, Spain:
    [Google Scholar]
  19. Owen R. J., Pitcher D. 1985 Current methods for estimating DNA base composition and levels of DNA-DNA hybridization. 67–93 Goodfellow M., Minnikin E.ed Chemical methods in bacterial systematics Academic Press Inc. Ltd.; London:
    [Google Scholar]
  20. Paillard C., Maes P. 1990; Etiologie de la maladie de fanneau brun chez Tapes phillipinarum’. pathogenicite d’un Vibrio sp.. Acad C. R. Sci. (Paris) 310:15–20
    [Google Scholar]
  21. Paillard C., Percelay L., Le Pennec M., Picard D. L. 1989; Origine pathogene de l“anneau brun” chez Tapes philippinarum (Mollusque, bivalve). Acad C. R. Sci. (Paris) 309:235–241
    [Google Scholar]
  22. Pujalte M. J., Ortiz-Conde B. A., Steven S. E., Esteve C., Garay E., Colwell R. R. 1992; Numerical taxonomy and nucleic acid studies of Vibrio mediterranei. Syst. Appl. Microbiol 15:82–91
    [Google Scholar]
  23. Robledo J. A., Caceres-Martinez J., Figueras A. 1994 The presence of the brown ring disease in clams from Galicia (NW of Spain) and Aveiro (W of Portugal). 29 Sixth International Colloqium on Pathology in Marine Aquaculture PAMAQ; Montpellier, France:
    [Google Scholar]
  24. Rohf F. J. 1992 NTSYS-PC Numerical taxonomy and multivariate analysis system; version 1.70. Exeter Software, Applied Biostatistics Inc., New York:
    [Google Scholar]
  25. Schiewe M. H., Trust T. J., Crosa J. H. 1981; Vibrio ordalii sp. nov.: a causative agent of vibriosis in fish. Curr. Microbiol 6:343–348
    [Google Scholar]
  26. Smibert R. M., Krieg N. R. 1981 General characterization. 409–443 Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B.ed Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  27. Sneath P. H. A. 1972; Computer taxonomy. Methods Microbiol 7A:29–98
    [Google Scholar]
  28. Sneath P. H. A., Johnson R. 1972; The influence on numerical taxonomic similarities of errors in microbiological tests. J. Gen. Microbiol 72:377–392
    [Google Scholar]
  29. Sneath P. H. A., Sokal R. R. 1973 Numerical taxonomy: the principles and practice of numerical classification. 188–308 W. H; Freeman & Co., San Francisco:
    [Google Scholar]
  30. Sutton D. C., Garrick R. 1993; Bacterial disease of cultured giant clam Tridacna gigas larvae. Dis. Aquat. Org 16:47–53
    [Google Scholar]
  31. Toranzo A. E., Baija J. L. 1990; A review of the taxonomy and seroepizootiology of Vibrio anguillarum, with special reference to aquaculture in Northwest of Spain. Dis. Aquat. Org 9:73–82
    [Google Scholar]
  32. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. L., Moore L. H., Murray R. G. E., Stackebrandt E., Starr M. P., Triiper H. G. 1987; Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Int. J. Syst. Bacteriol 37:463–464
    [Google Scholar]
  33. West P. A., Colwell R. R. 1984 Identification and classification of Vibrionaceae, An overview. 285–363 Colwell R. R.ed Vibrios in the environment John Wiley & Sons, Inc.; New York:
    [Google Scholar]
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