A bacterium isolated from a polluted stream, capable of metabolizing biphenyl, naphthalene, phenanthrene, and higher-molecular-weight polycyclic aromatic hydrocarbons (D. Gibson, V. Mahadevan, D. Jerina, H. Yagi, and H. Yeh, Science 189:295–297, 1975), was previously identified as sp. strain B1. In this investigation, 16S rRNA gene sequencing, biochemical tests, fatty acid methyl ester analysis, polyacrylamide gel electrophoresis of protein, and DNA-DNA hybridization were used to determine the taxonomic relationship of sp. strain B1. The sequence of the 16S rRNA gene of B1 was identical to that of ATCC 51230. The biochemical tests, fatty acid analysis, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis profile of soluble proteins of strain B1 showed results similar to those of . DNA-DNA hybridization indicated that B1 and ATCC 51230 are 75% homologous at the DNA level. We propose that sp. strain B1 be reclassified as .


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