1887

Abstract

Organisms with the typical characteristics of mycoplasmas were isolated from the genital tracts of female elephants. The results of growth inhibition tests, metabolic inhibition tests, indirect immunofluorescence tests, and immunobinding assays showed that the isolated mycoplasmas were identical and distinct from previously described , and species. These organisms represent a new species, for which the name is proposed. ferments glucose, fructose, maltose, mannose, and sucrose, produces films and spots, does not hydrolyze arginine, esculin, and urea, does not reduce methylene blue, tetrazolium chloride, and potassium tellurite, does not possess phosphatase activity, and reduces resazurin. It lyses avian, ovine, and guinea pig erythrocytes. It does not adsorb erythrocytes. Cholesterol or serum is required for growth. The optimum growth temperature is 37°C. The G+C content of the DNA is 24.0 mol%. The type strain of is E42 (= ATCC 51980).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-46-2-437
1996-04-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/46/2/ijs-46-2-437.html?itemId=/content/journal/ijsem/10.1099/00207713-46-2-437&mimeType=html&fmt=ahah

References

  1. Aluotto B. B., Wittier R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologic techniques for the characterization of Mycoplasma species. Int. J. Syst. Bacteriol 20:35–58
    [Google Scholar]
  2. Barber T. L., Fabricant J. 1971; Identification of Mycoplasmatales-. characterization procedures. Appl. Microbiol 21:600–605
    [Google Scholar]
  3. Clark H. W., Bailey J. S., Laughlin D. C., Brown T. M. 1978; Isolation of mycoplasma from the genital tracts of elephants. Zentralbl Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig 241:262
    [Google Scholar]
  4. Clark H. W., Laughlin D. C., Bailey J. S., Brown T. M. 1980; Mycoplasma species and arthritis in elephants. J. Zoo Anim. Med 11:3–15
    [Google Scholar]
  5. Clyde W. A. Jr. 1964; Mycoplasma species identification based upon growth inhibition by specific antisera. J. Immunol 92:958–965
    [Google Scholar]
  6. Del Giudice R. A., Robillard F., Carski T. R. 1967; Immunofluorescence identification of mycoplasma on agar by use of incident illumination. J. Bacteriol 93:1205–1209
    [Google Scholar]
  7. Edward D. G. ff 1971; Determination of sterol requirement for Mycoplasmatales. J. Gen. Microbiol 69:205–210
    [Google Scholar]
  8. Freundt E. A., Andrews B. E., Erno H., Kunze M., Black F. T. 1973; The sensitivity of Mycoplasmatales to sodium-polyanethol sulfonate and digitonin. Zentralbl, Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe A 225:104–112
    [Google Scholar]
  9. Gross-Bellard M. J., Oudet P., Chambon P. 1973; Isolation of high molecular weight DNA from mammalian cells. Eur. J. Biochem 36:32–38
    [Google Scholar]
  10. Hill A. C. 1971; Mycoplasma caviae, a new species. J. Gen. Microbiol 65:109–113
    [Google Scholar]
  11. Hill A. C. 1977; The metabolic inhibition test for mycoplasmas based on phosphatase production. J. Hyg 79:391–393
    [Google Scholar]
  12. Kirchhoff H., Beyene P., Fischer M., Flossdorf J., Heitmann J., Khattab B., Lopatta D., Rosengarten R., Seidel G., Yousef C. 1987; Mycoplasma mobile sp. nov., a new species from fish. Int. J. Syst. Bacteriol 37:192–197
    [Google Scholar]
  13. Kotani H., McGarrity G. J. 1985; Rapid and simple identification of mycoplasmas by immunobinding. J. Immunol. Methods 85:257–267
    [Google Scholar]
  14. Manchee R. J., Taylor-Robinson D. 1968; Haemadsorption and haemagglutination by mycoplasmas. J. Gen. Microbiol 50:465–478
    [Google Scholar]
  15. Morton H. E., Roberts R. J. 1967; Production of anti-mycoplasma (PPLO) antibodies in rabbits. Proc. Soc. Exp. Biol. Med 125:538–543
    [Google Scholar]
  16. Polak-Vogelzang A. A., Hagenaars R., Nagel S. 1978; Evaluation of an indirect immunoperoxidase test for identification of Acholeplasma and Mycoplasma. J. Gen. Microbiol 106:241–249
    [Google Scholar]
  17. Purcell R. H., Taylor-Robinson D., Wong D. C., Chanock R. M. 1966; A color test for the measurement of antibody to the non-acid-forming human mycoplasma species. Am. J. Epidemiol 84:51–66
    [Google Scholar]
  18. Razin S., Tully J. G. 1970; Cholesterol requirement of mycoplasmas. J. Bacteriol 92:6–12
    [Google Scholar]
  19. Rosengarten R., Behrens A., Stetefeld A., Heller M., Ahrens M., Sachse K., Yogev D., Kirchhoff H. 1994; Antigen heterogeneity among isolates of Mycoplasma bovis is generated by high-frequency variation of diverse membrane surface proteins. Infect. Immun 62:5066–5074
    [Google Scholar]
  20. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J. Mol. Biol 4:430–443
    [Google Scholar]
  21. Subcommittee on the Taxonomy of Mollicutes of the International Committee on Systematic Bacteriology 1995; Revised minimum standards for description of new species of the class Mollicutes (division Tenericutes). Int. J, Syst, Bacteriol 45:605–612
    [Google Scholar]
  22. Subcommittee on the Taxonomy of Mollicutes, 1988; Minutes of the interim meeting, 25 and 28 August 1986, Birmingham, Alabama. Int. J. Syst. Bacteriol 38:226–230
    [Google Scholar]
  23. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. 1966; A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J. Hyg 64:91–104
    [Google Scholar]
  24. Taylor-Robinson D., Williams M. H., Haig D. A. 1968; The isolation and comparative biological and physical characteristics of T-mycoplasmas of cattle. J. Gen. Microbiol 54:33–46
    [Google Scholar]
  25. Williams C. O., Wittier R. G. 1971; Hydrolysis of esculin and phosphatase production by members of the order Mycoplasmatales which do not require sterol, Int. J. Syst. Bacteriol 21:73–77
    [Google Scholar]
/content/journal/ijsem/10.1099/00207713-46-2-437
Loading
/content/journal/ijsem/10.1099/00207713-46-2-437
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error