1887

Abstract

Phenotypic and genetic traits of porcine intestinal spirochete strain P43/6/78 (= ATCC 51139) (T = type strain), which is pathogenic and weakly beta-hemolytic, were determined in order to confirm the taxonomic position of this organism and its relationships to previously described species of intestinal spirochetes. In BHIS broth, P43/6/78 cells had a doubling time of 1 to 2 h and grew to a maximum cell density of 2 x 10 cells per ml at 37 to 42°C. They hydrolyzed hippurate, utilized -glucose, -fructose, sucrose, -trehalose, -galactose, -mannose, maltose, -acetyl--glucosamine, -glucosamine, pyruvate, -fucose, -cellobiose, and -ribose as growth substrates, and produced acetate, butyrate, ethanol, H, and CO as metabolic products. They consumed substrate amounts of oxygen and had a G+C content (24.6 mol%) similar to that of B78 (25.9 mol%). Phenotypic traits that could be used to distinguish strain P43/6/78 from and included its ultrastructural appearance (each strain P43/6/78 cell had 8 or 10 periplasmic flagella, with 4 or 5 flagella inserted at each end, and the cells were thinner and shorter and had more pointed ends than and cells), its faster growth rate in liquid media, its hydrolysis of hippurate, its lack of β-glucosidase activity, and its metabolism of -ribose. DNA-DNA relative reassociation experiments in which the S1 nuclease method was used revealed that P43/6/78 was related to, but was genetically distinct from, both B78 (level of sequence homology, 25 to 32%) and B256 (level of sequence homology, 24 to 25%). These and previous results indicate that intestinal spirochete strain P43/6/78 represents a distinct species. Therefore, we propose that strain P43/6/78 should be designated as the type strain of a new species,

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1996-01-01
2024-12-06
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References

  1. Andrews J. J., Hoffman L. J. 1982; A porcine colitis caused by a weakly p-hemolytic treponeme (Treponema innocens?),. 395–402Proceedings of the 25th American Association of Veterinary Laboratory DiagnosticiansNashville, Tenn
    [Google Scholar]
  2. Duhamel G. E., Hunsaker B. D., Mathieson M. R., Moxley R. A. Intestinal spirochaetosis and giardiasis in a beagle pup with diarrhoea. Vet. Pathol in press
    [Google Scholar]
  3. Duhamel G. E., Muniappa N., Mathieson M. R., Johnson J. L., Toth J., Elder R. O., Doster A. R. 1995; Certain canine weakly p-hemolytic intestinal spirochetes are phenotypically and genotypically related to spirochetes associated with human and porcine intestinal spirochetosis. J. Clin. Microbiol 33:2212–2215
    [Google Scholar]
  4. Duhamel G. E., Ramanathan M., Gardner I., Anderson M. A., Walker R. L., Hampson D. J. 1993; Intestinal spirochetosis of swine associated with weakly p-hemolytic spirochetes distinct from Serpulina innocens,. 53Proceedings of the 36th American Association of Veterinary Laboratory DiagnosticiansLas Vegas, Nev
    [Google Scholar]
  5. Fellstrom C., Gunnarsson A. 1995; Phenotypic characterisation of intestinal spirochaetes isolated from pigs. Res. Vet. Sci 59:1–4
    [Google Scholar]
  6. Fellstrom C., Pettersson B., Uhlen M., Gunnarsson A., Johansson K. 1995; Phylogeny of Serpulina based on sequence analyses of the 16S rRNA gene and comparison with a scheme involving biochemical classification. Res. Vet. Sci 59:5–9
    [Google Scholar]
  7. Girard C., Lemarchand T., Higgins R. 1995; Porcine colonic spirochetosis: a retrospective study of eleven cases. Can. Vet. J 36:291–294
    [Google Scholar]
  8. Harland W. A., Lee F. D. 1967; Intestinal spirochaetosis. Br. Med. J 3:718–719
    [Google Scholar]
  9. Holdeman L. V., Cato E. P., Moore W. E. C.ed 1977 Anaerobic laboratory manual Anaerobe Laboratory; Virginia Polytechnic Institute and State University, Blacksburg:
    [Google Scholar]
  10. Hookey J. V., Barrett S. P., Read C. S., Barber P. 1994; Phylogeny of the human intestinal spirochetes inferred from 16S rDNA sequence comparisons. FEMS Microbiol. Lett 117:345–350
    [Google Scholar]
  11. Hovind-Hougen K., Birch-Andersen A., Henrik-Nielsen R., Orholm M., Pedersen J. O., Teglbjaerg P. S., Thaysen E. H. 1982; Intestinal spirochetosis: morphological characterization and cultivation of the spirochete Brachyspira aalborgi gen. nov., sp. nov. J. Clin. Microbiol 16:1127–1136
    [Google Scholar]
  12. Hovind-Hougen K., HOgh P., Birch-Andersen A. 1990; Morphological heterogeneity among spirochetes isolated from cases of swine dysentery. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig 274:1–15
    [Google Scholar]
  13. Hunter D., Wood T. 1979; An evaluation of the API ZYM system as a means of classifying spirochaetes associated with swine dysentery. Vet. Rec 104:383–384
    [Google Scholar]
  14. Jacques C., Girard C., Higgins R., Goyette G. 1989; Extensive colonization of the porcine colonic epithelium by a spirochete similar to Treponema innocens. J. Clin. Microbiol 27:1139–1141
    [Google Scholar]
  15. Johnson J. L. 1985; DNA reassociation and RNA hybridization of bacterial nucleic acids. Methods Microbiol 18:33–74
    [Google Scholar]
  16. Johnson J. L. 1994 Similarity analysis of DNAs. 655–682 Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.ed Methods for general and molecular bacteriology American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  17. Johnson J. L., Phelps C. F., Cummins C. S., London J., Gasser F. 1980; Taxonomy of the Lactobacillus acidophilus group. Int. J. Syst. Bacteriol 30:53–68
    [Google Scholar]
  18. Kinyon J. M., Harris D. L. 1979; Treponema innocens, a new species of intestinal bacteria, and emended description of the type strain of Treponema hyodysenteriae. Harris. et al. Int. J. Syst. Bacterio1 29:102–109
    [Google Scholar]
  19. Kitai K., Kashiwazaki M., Adachi Y., Kume T., Arakawa A. 1979; In vitro activity of 39 antimicrobial agents against Treponema hyodysenteriae. Antimicrob. Agents Chemother 15:392–395
    [Google Scholar]
  20. Kleiner D. E., Stetler-Stevenson W. G. 1994; Quantitative zymography: detection of picogram quantities of gelatinases. Anal. Biochem 218:325–329
    [Google Scholar]
  21. Kunkle R. A., Kinyon J. M. 1988; Improved selective medium for the isolation of Treponema hyodysenteriae. J. Clin. Microbiol 26:2357–2360
    [Google Scholar]
  22. Lee F. D., Kraszewski A., Gordon J., Howie J. G. R., McSeveney D., Harland W. A. 1971; Intestinal spirochaetosis. Gut 12:126–133
    [Google Scholar]
  23. Lee J. L, Hampson D. J. 1994; Genetic characterisation of intestinal spirochaetes, and their association with disease. J. Med. Microbiol 40:365–371
    [Google Scholar]
  24. Lee J. L., Hampson D. J., Combs B. G., Lymbery A. J. 1993; Genetic relationships between isolates of Serpulina (Treponema) hyodysenteriae, and comparison of methods for their subspecific differentiation. Vet. Microbiol 34:3546
    [Google Scholar]
  25. Lee J. I., Hampson D. J., Lymbery A. J., Harders S. J. 1993; The porcine intestinal spirochaetes: identification of new genetic groups. Vet. Microbiol 34:273–285
    [Google Scholar]
  26. Lee J. L, McLaren A. J., Lymbery A. J., Hampson D. J. 1993; Human intestinal spirochaetes are distinct from Serpulina hyodysenteriae. J. Clin. Microbiol 31:16–21
    [Google Scholar]
  27. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12:195–206
    [Google Scholar]
  28. McLaren A. J., Trott D. J., Swayne D. E., Stoutenburg J. W., Hampson D. J. 1994; Characterization of avian intestinal spirochetes. 66Proceedings of the North Central Avian Disease CongressDes Moines, Iowa
    [Google Scholar]
  29. Park N. Y., Chung C. Y., McLaren A. J., Atyeo R. F., Hampson D. J. 1995; Polymerase chain reaction for identification of human and porcine spirochaetes recovered from cases of intestinal spirochaetosis. FEMS Microbiol. Lett 125:225–230
    [Google Scholar]
  30. Ramanathan M., Duhamel G. E., Mathiesen M. R., Messier S. 1993; Identification and partial characterisation of a group of weakly p-hemolytic intestinal spirochetes of swine distinct from Serpulina innocens isolate B256. Vet. Microbiol 37:53–64
    [Google Scholar]
  31. Spearman J. G., Nayar G., Sheridan M. 1988; Colitis associated with Treponema innocens in pigs. Can. Vet. J 29:747
    [Google Scholar]
  32. Stanton T. B. 1989; Glucose metabolism and NADH recycling by Treponema hyodysenteriae, the agent of swine dysentery. Appl. Environ. Microbiol 55:2365–2371
    [Google Scholar]
  33. Stanton T. B. 1992; Proposal to change the genus designation Serpulina to Serpulina gen. nov. containing the species Serpulina hyodysenteriae comb, nov. and Setpulina innocens comb. nov. Int. J. Syst. Bacteriol 42:189–192
    [Google Scholar]
  34. Stanton T. B., Hanzelka B. L., Jensen N. S. 1995; Survey of intestinal spirochetes for NADH oxidase activity. Microb. Ecol. Health Dis 8:93–100
    [Google Scholar]
  35. Stanton T. B., Jensen N. S., Casey T. A., Tordoff L. A., Dewhirst F. E., Paster B. J. 1991; Reclassification of Treponema hyodysenteriae and Treponema innocens in a new genus, Serpula gen. nov., as Serpula hyodysenteriae comb. nov. and Serpula innocens comb. nov. Int. J. Syst. Bacteriol 41:50–58
    [Google Scholar]
  36. Stanton T. B., Lebo D. F. 1988; Treponema hyodysenteriae growth under various culture conditions. Vet. Microbiol 18:177–190
    [Google Scholar]
  37. Stanton T. B., Savage D. C. 1983; Roseburia cecicola gen. nov., sp. nov., a motile, obligately anaerobic bacterium from a mouse cecum. Int. J. Syst. Bacteriol 33:618–627
    [Google Scholar]
  38. Stanton T. B., Trott D. J., Lee J. I., McLaren A. J., Hampson D. J., Paster B. J., Jensen N. S. Differentiation of intestinal spirochaetes by multilocus enzyme electrophoresis and 16S rRNA comparisons. Submitted for publication
  39. Taylor D. J. 1992 Spirochaetal diarrhoea. 584–587 Leman A. D., Straw B. E., Mengeling W. L., D’Allaire S., Taylor D. J.ed Diseases of swine Iowa State University Press; Ames:
    [Google Scholar]
  40. Taylor D. J., Simmons J. R., Laird H. M. 1980; Production of diarrhoea and dysentery in pigs by feeding pure cultures of a spirochaete differing from Treponema hyodysenteriae. Vet. Rec 106:326–332
    [Google Scholar]
  41. Trott D. J., McLaren A. J., Hampson D. J. 1995; Pathogenicity of human and porcine intestinal spirochetes in day-old specific-pathogen-free chicks: an animal model of intestinal spirochetosis. Infect. Immun 63:3705–3710
    [Google Scholar]
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