1887

Abstract

Seven similar strains which were pathogens of Japanese flounder larvae with opaque intestines had characteristics of the genus . These strains were divided into two genomic species (species 1 containing six strains, and species 2 containing one strain) on the basis of the results of DNA-DNA hybridization experiments in which the membrane filter method was used, and these two species could be differentiated from each other by the following characteristics: acid production from -galactose and utilization of -glucuronate and β-hydroxybutyrate. Strain F-2, the type strain of species 1, exhibited levels of DNA relatedness with 29 previously described species of 5 to 18%. The flounder isolates belonging to species 1 were also differentiated from the previously described species phenotypically by the following characteristics: they were nitrate reduction positive; each cell had a single polar flagellum; they did not produce arginine dihydrolase, chitinase, gelatinase, and lipase; they did not utilize -cellobiose and citrate; and they did not grow at 35°C. The G+C contents of the DNAs of four species 1 strains were 43 to 44 mol%. The name sp. nov. is proposed for genomic species 1. The type strain of is strain F-2 (= IFO 15847). Species 2 was also considered a new genomic species, but a species name is not proposed in this paper because only one strain is available and the phenotypic variability of the species is not known.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-46-1-155
1996-01-01
2022-09-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/46/1/ijs-46-1-155.html?itemId=/content/journal/ijsem/10.1099/00207713-46-1-155&mimeType=html&fmt=ahah

References

  1. Aaronson S. 1970 Experimental microbial ecology. 70 Academic Press; New York:
    [Google Scholar]
  2. Akagawa-Matsushita M., Matsuo M., Koga Y., Yamasato K. 1992; Alteromonas atlantica sp. nov. and Alteromonas carrageenovora sp. nov., bacteria that decompose algal polysaccharides. Int. J. Syst. Bacteriol 42:621–627
    [Google Scholar]
  3. Citarella R. V., Colwell R. R. 1970; Polyphasic taxonomy of the genus Vibrio: polynucleotide sequence relationships among selected Vibrio species. J. Bacteriol 104:434–442
    [Google Scholar]
  4. Davis B. R., Fanning G. R., Madden J. M., Steigerwalt A. G., Bradford H. B. Jr., Smith H. L. Jr., Brenner D. J. 1981; Characterization of biochemically atypical Vibrio cholerae strains and designation of a new pathogenic species, Vibrio mimicus. J. Clin. Microbiol 14:631–639
    [Google Scholar]
  5. Farmer J. J. III, Hickman-Brenner F. W. 1992 The genera Vibrio and Photobacterium,. 2952–3011 Balows A., Triiper H. G., Dworkin M., Harder W., Schleifer K.-H.ed The prokaryotes, 2nd. Springer-Verlag; New York:
    [Google Scholar]
  6. Grimes D. J., Stemmier J., Hada H., May E. B., Maneval D., Hetrick F. M., Jones R. T., Stoskopf M., Colwell R. R. 1984; Vibrio species associated with mortality of sharks held in captivity. Microb. Ecol 10:271–282
    [Google Scholar]
  7. Hickman F. W., Farmer J. J. III, Hollis D. G., Fanning G. R., Steigerwalt A. G., Weaver R. E., Brenner D. J. 1982; Identification of Vibrio hollisae sp. nov. from patients with diarrhea. J. Clin. Microbiol 15:395–401
    [Google Scholar]
  8. Holt J. G., Krieg N. R., Sneath P. H. A., Staley J. T., Williams S. T.ed 1994 Bergey’s manual of determinative bacteriology. , 9th.192–194260–274 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  9. Ishimaru K., Akagawa-Matsushita M., Muroga K. 1995; Vibrio penaeicida sp. nov., a pathogen of kuruma prawns (Penaeus japonicus). Int. J. Syst. Bacteriol 45:134–138
    [Google Scholar]
  10. Kumagai M., Fupimoto M., Kuninaka A. 1988; Determination of base composition of DNA by high performance liquid chromatography of its nuclease Pl hydrolysate. Nucleic Acids Symp. Ser 19:65–68
    [Google Scholar]
  11. Masumura K., Yasunobu H., Okada N., Muroga K. 1989; Isolation of a Vibrio sp., the causative bacterium of intestinal necrosis of Japanese flounder larvae. Fish Pathol 24:135–141 In Japanese
    [Google Scholar]
  12. Murata O. 1987; An infectious disease of larval flounder showing opaque intestine. Fish Pathol 22:59–61 In Japanese
    [Google Scholar]
  13. Muroga K. 1992 Hatchery diseases of marine fish in Japan. 215–222 Shariff M., Subasinghe R. P., Arthur J. R.ed Diseases in Asian aquaculture I Fish Health Section, Asian Fisheries Society; Manila, The Philippines:
    [Google Scholar]
  14. Muroga K., Yasunobu H., Okada N., Masumura K. 1990; Bacterial enteritis of cultured flounder Paralichthys olivaceus larvae. Dis. Aquat. Org 9:121–125
    [Google Scholar]
  15. Onarheim A. M., Wiik R., Burghardt J., Stackebrandt E. 1994; Characterization and identification of two Vibrio species indigenous to the intestine of fish in cold sea water; description of Vibrio iliopiscarius sp. nov. Syst. Appl. Microbiol 17:370–379
    [Google Scholar]
  16. Pujalte M. J., Ortigosa M., Urdaci M. C., Garay E., Grimont P. A. D. 1993; Vibrio mytili sp. nov., from mussels. Int. J. Syst. Bacteriol 43:358–362
    [Google Scholar]
  17. Tison D. L., Nishibuchi M., Greenwood J. D., Seidler R. J. 1982; Vibrio vulnificus biogroup 2: new biogroup pathogenic for eels. Appl. Environ. Microbiol 44:640–646
    [Google Scholar]
  18. Urdaci M. C., Marchand M., Ageron E., Arcos J. M., Sesma B., Grimont P. A. D. 1991; Vibrio navarrensis sp. nov., a species from sewage. Int. J. Syst. Bacteriol 41:290–294
    [Google Scholar]
  19. West P. A., Colwell R. R. 1984 Identification and classification of Vibrionaceae–an overview. 285–363 Colwell R. R.ed Vibrios in the environment John Wiley & Sons, Inc.; New York:
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-46-1-155
Loading
/content/journal/ijsem/10.1099/00207713-46-1-155
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error