Seven similar strains which were pathogens of Japanese flounder larvae with opaque intestines had characteristics of the genus . These strains were divided into two genomic species (species 1 containing six strains, and species 2 containing one strain) on the basis of the results of DNA-DNA hybridization experiments in which the membrane filter method was used, and these two species could be differentiated from each other by the following characteristics: acid production from D-galactose and utilization of D-glucuronate and β-hydroxybutyrate. Strain F-2, the type strain of species 1, exhibited levels of DNA relatedness with 29 previously described species of 5 to 18%. The flounder isolates belonging to species 1 were also differentiated from the previously described species phenotypically by the following characteristics: they were nitrate reduction positive; each cell had a single polar flagellum; they did not produce arginine dihydrolase, chitinase, gelatinase, and lipase; they did not utilize D-cellobiose and citrate; and they did not grow at 35°C. The G+C contents of the DNAs of four species 1 strains were 43 to 44 mol%. The name sp. nov. is proposed for genomic species 1. The type strain of is strain F-2 (= IFO 15847). Species 2 was also considered a new genomic species, but a species name is not proposed in this paper because only one strain is available and the phenotypic variability of the species is not known.


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