1887

Abstract

A comprehensive DNA-DNA hybridization study was performed by using 183 strains of the genus . This genus was shown to comprise 20 DNA homology groups which are considered genomic species. Four groups corresponded to the previously described species , and . The previously described species was heterogeneous and was divided into 16 DNA homology groups. One of these groups exhibited a high level of DNA homology with . The 62 pathovars represented in this study were allocated to appropriate species. Our results, together with previous taxonomic data, supported a comprehensive revision of the classification of the genus . The species , and are not affected. The type species of the genus, (Pammel 1895) Dowson 1939, is emended to include only the pathovars obtained from crucifers (i.e., pv. aberrans, pv. armoraciae, pv. barbareae, pv. campestris, pv. incanae, and pv. raphani). Starr and Garces 1950 is emended to include 34 former pathovars. The following species names are proposed: sp. nov., including pv. corylina, pv. juglandis, pv. poinsettiicola (type C strains of the former pathovar), pv. populi, and pv. pruni; sp. nov. for strains isolated from bromegrass; (ex Wiehe and Dowson 1953) sp. nov., nom. rev.; sp. nov., including type B strains of the former taxon pv. poinsettiicola; (ex Bryan 1926) sp. nov., nom. rev.; sp. nov., including pv. hederae, pv. pelargonii, and pv. vitians; (ex Wakker 1883) sp. nov., nom. rev.; sp. nov.; (ex Goto and Okabe 1958) sp. nov., nom. rev.; sp. nov. for strains isolated from diseased sugarcane in Guadeloupe; sp. nov.; (ex Jones, Johnson, and Reddy 1917) sp. nov., nom. rev., including pv. arrhenatheri, pv. cerealis, pv. graminis, pv. hordei, pv. phlei, pv. phleipratensis, pv. poae, pv. secalis, pv. translucens, and pv. undulosa; sp. nov., including pv. holcicola and pv. vasculorum (type B strains of the former taxon pv. vasculorum); and (ex Doidge 1920) sp. nov., nom. rev., which includes the type B strains of the former taxon pv. vesicatoria. Differentiating characteristics were determined for the new species on the basis of metabolic activity on a range of carbon substrates by using the Biolog GN microplate system.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-45-3-472
1995-07-01
2022-07-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/45/3/ijs-45-3-472.html?itemId=/content/journal/ijsem/10.1099/00207713-45-3-472&mimeType=html&fmt=ahah

References

  1. Andrewes A. G., Jenkins C. L., Starr M. P., Shepherd J., Hope H. 1976; Structure of xanthomonadin I, a novel dibrominated arylpolyene pigment produced by the bacterium Xanthomonas juglandis . Tetrahedron Lett. 45:4023–4024
    [Google Scholar]
  2. Auling G., Busse H.-J., Pilz F., Webb L., Kneifel H., Claus D. 1991; Rapid differentiation, by polyamine analysis, of Xanthomonas strains from phytopathogenic pseudomonads and other members of the class Proteobacteria interacting with plants. Int. J. Syst. Bacteriol. 41:223–228
    [Google Scholar]
  3. Azad H., Schaad N. W. 1988; The relationship of Xanthomonas campestris pv. translucens to frost and the effect of frost on black chaff development in wheat. Phytopathology 78:95–100
    [Google Scholar]
  4. Berthier Y., Verdier V., Guesdon J.-L., Chewier D., Denis J.-B., Decoux G., Lemattre M. 1993; Characterization of Xanthomonas campestris pathovars by rRNA gene restriction patterns. Appl. Environ. Microbiol. 59:851–859
    [Google Scholar]
  5. Bochner B. 1989; Sleuthing out bacterial identities. Nature (London) 339:157–158
    [Google Scholar]
  6. Bouzar H., Jones J. B., Stall R. E., Hodge N. C., Minsavage G. V., Benedict A. A., Alvarez A. M. 1994; Physiological, chemical, serological, and pathogenic analyses of a worldwide collection of Xanthomonas campestris pv. vesicatoria strains. Phytopathology 84:663–671
    [Google Scholar]
  7. Bradbury J. F. 1984; Genus II. Xanthomonas Dowson 1939, 187AL,. 199–210 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology, 1 Williams & Wilkins; Baltimore:
    [Google Scholar]
  8. Bradbury J. F. 1986; Xanthomonas Dowson 1939,187,. 198–260 Guide to plant pathogenic bacteria CAB International Mycological Institute; Slough, United Kingdom:
    [Google Scholar]
  9. Bryan M. K. 1926; Bacterial leafspot on hubbard squash. Science 63:165
    [Google Scholar]
  10. Colwell R. R., Liston J. 1961; Taxonomic analysis with the electronic computer of some Xanthomonas and Pseudomonas species. J. Bacteriol. 82:913–919
    [Google Scholar]
  11. Cunfer B. M., Scolari B. L. 1982; Xanthomonas campestris pv. translucens on triticale and other small grains. Phytopathology 72:683–686
    [Google Scholar]
  12. De Ley J. 1970; Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J. Bacteriol. 101:738–754
    [Google Scholar]
  13. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem. 12:133–142
    [Google Scholar]
  14. De Vos P., De Ley J. 1983; Intra- and intergeneric similarities of Pseudomonas and Xanthomonas ribosomal ribonucleic acid cistrons. Int. J. Syst. Bacteriol. 33:487–509
    [Google Scholar]
  15. Doidge E. M. 1920; A tomato cancer. J. Dep. Agric. Union S. Afr. 1:718–721
    [Google Scholar]
  16. Dowson W. J. 1939; On the systematic position and generic names of the Gram negative bacterial plant pathogens. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 2 100:177–193
    [Google Scholar]
  17. Dye D. W. 1962; The inadequacy of the usual determinative tests for the identification of Xanthomonas spp. N. Z. J. Sci. 5:393–416
    [Google Scholar]
  18. Dye D. W., Bradbury J. F., Goto M., Hayward A. G., Lelliott R. A., Schroth M. N. 1980; International standards for naming pathovars of phytopathogenic bacteria and a list of pathovar names and pathotype strains. Rev. Plant Pathol. 59:153–168
    [Google Scholar]
  19. Dye D. W., Lelliott R. A. 1974; Genus II. Xanthomonas, . 243–249 Buchanan R. E., Gibbons N. E. Bergey’s manual of determinative bacteriology,, 8. Williams & Wilkins; Baltimore:
    [Google Scholar]
  20. Egel D. S., Graham J. H., Stall R. E. 1991; Genomic relatedness of Xanthomonas campestris strains causing diseases of citrus. Appl. Environ. Microbiol. 57:2724–2730
    [Google Scholar]
  21. Gabriel D. W., Hunter J. E., Kingsley M. T., Miller J. W., Lazo G. R. 1988; Clonal population structure of Xanthomonas campestris and genetic diversity among citrus canker strains. Mol. Plant Microbe Interact. 1:59–65
    [Google Scholar]
  22. Gabriel D. W., Kingsley M. T., Hunter J. E., Gottwald T. 1989; Reinstatement of Xanthomonas citri (ex Hasse) and X. phaseoli (ex Smith) to species and reclassification of all X. campestris pv. citri strains. Int. J. Syst. Bacteriol. 39:14–22
    [Google Scholar]
  23. Goto M., Okabe N. 1958; Bacterial plant diseases in Japan. IX. 1. Bacterial stem rot of pea. 2. Halo blight of bean. 3. Bacterial spot of physalis plant. Rep. Fac. Agric. Shizuoka Univ. 8:33–49
    [Google Scholar]
  24. Hartung J. S., Civerolo E. L. 1987; Genomic fingerprints of Xanthomonas campestris pv. citri strains from Asia, South America, and Florida. Phytopathology 77:282–285
    [Google Scholar]
  25. Hartung J. S., Civerolo E. L. 1989; Restriction fragment length polymorphisms distinguish Xanthomonas campestris strains isolated from Florida citrus nurseries from X c. pv. citri. Phytopathology 79:793–799
    [Google Scholar]
  26. Hayward A. C. 1993; The hosts of Xanthomonas, . 1–119 Swings J. G., Civerolo E. L. Xanthomonas Chapman & Hall; London:
    [Google Scholar]
  27. Hildebrand D. C., , Hendson M., Schroth M. N. 1993; Usefulness of nutritional screening for the identification of Xanthomonas campestris DNA homology groups and pathovars. J. Appl. Bacteriol. 75:447–455
    [Google Scholar]
  28. Hildebrand D. C., , Palleroni N. J., Schroth M. N. 1990; Deoxyribonucleic acid relatedness of 24 xanthomonad strains representing 23 Xanthomonas campestris pathovars and Xanthomonas fragariae . J. Appl. Bacteriol. 68:263–269
    [Google Scholar]
  29. Johnson J. L. 1984; Nucleic acids in bacterial classification,. 8–11 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology, 1 Williams & Wilkins; Baltimore:
    [Google Scholar]
  30. Jones J. B., Chase A. R., Harris G. K. 1993; Evaluation of the Biolog GN MicroPlate system for identification of some plant-pathogenic bacteria. Plant Dis. 77:553–558
    [Google Scholar]
  31. Jones L. R., Johnson A. G., Reddy C. S. 1917; Bacterial blight of barley. J. Agric. Res. 11:625–644
    [Google Scholar]
  32. Lapage S. P., Sneath P. H. A., Lessel E. F., Skerman Y. B. D., Seeliger H. P. R., Clark W. A. 1975; International code of nomenclature of bacteria. 1976 Revision. American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  33. Lazo G. R., Roffey R., Gabriel D. W. 1987; Pathovars of Xanthomonas campestris are distinguishable by restriction fragment length polymorphism. Int. J. Syst. Bacteriol. 37:214–221
    [Google Scholar]
  34. Lee Y.-A., Hildebrand D. C., Schroth M. N. 1992; Use of quinate metabolism as a phenotypic property to identify members of Xanthomonas campestris DNA homology group 6. Phytopathology 82:971–973
    [Google Scholar]
  35. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  36. Murata G. R., Starr M. P. 1973; A concept of the genus Xanthomonas and its species in the light of segmental homology of deoxyribonucleic acids. Phytopathol. Z. 77:285–323
    [Google Scholar]
  37. Palleroni N. J., Bradbury J. F. 1993; Stenotrophomonas, a new bacterial genus for Xanthomonas maltophilia (Hugh 1980) Swings et al. 1983. Int. J. Syst. Bacteriol. 43:606–609
    [Google Scholar]
  38. Palleroni N. J., Hildebrand D. C., Schroth M. N., Hendson M. 1993; Deoxyribonucleic acid relatedness of 21 strains of Xanthomonas species and pathovars. J. Appl. Bacteriol. 75:441–446
    [Google Scholar]
  39. Schleifer K. H., Stackebrandt E. 1983; Molecular systematics of prokaryotes. Annu. Rev. Microbiol. 37:143–187
    [Google Scholar]
  40. Schroth M. N., Hildebrand O. C. 1983; Towards a sensible taxonomy of bacterial plant pathogens. Plant Dis. 67:128
    [Google Scholar]
  41. Stall R. E., Beaulieu C., Egel D., Hodge N. C., Leite R. P., Minsavage G. V., Bonzar H., Jones J. B., Alvarez A. M., Benedict A. A. 1994; Two genetically diverse groups of strains are included in Xanthomonas campestris pv. vesicatoria. Int. J. Syst. Bacteriol. 44:47–53
    [Google Scholar]
  42. Starr M. P., Garcés O. C. 1950; El agente causante de la gomosis bacterial del pasto imperial en Colombia. Rev. Fac. Nac. Agron. Medellin 12:73–83
    [Google Scholar]
  43. Swings J., De Vos P., Van den Mooter M., De Ley J. 1983; Transfer of Pseudomonas maltophilia Hugh 1981 to the genus Xanthomonas as Xanthomonas maltophilia (Hugh 1981) comb. nov. Int. J. Syst. Bacteriol. 33:409–413
    [Google Scholar]
  44. Swings J., Van den Mooter M., Vauterin L., Hoste B., Gillis M., Mew T. W., Kersters K. 1991; Reclassification of the causal agents of bacterial blight (Xanthomonas campestris pv. oryzae) and bacterial leaf streak (Xanthomonas campestris pv. oryzicola) of rice as pathovars of Xanthomonas oryzae (ex Ishiyama 1922) sp. nov., nom. rev. Int. J. Syst. Bacteriol. 40:301–311
    [Google Scholar]
  45. Van den Mooter M., Maraite H., Meiresonne L., Swings J., Gillis M., Kersters K., De Ley J. 1987; Comparison between Xanthomonas campestris pv. manihotis (ISPP List 1980) and X. campestris pv. cassavae (ISPP List 1980) by means of phenotypic, protein electrophoretic, DNA hybridization and phytopathological techniques. J. Gen. Microbiol. 133:57–71
    [Google Scholar]
  46. Van den Mooter M., Steenackers M., Maertens C., Gossele F., De Vos P., Swings J., Kersters K., De Ley J. 1987; Differentiation between Xanthomonas campestris pv. graminis (ISPP List 1980), pv. phleipratensis (ISPP List 1980) emend., pv. poae Egli and Schmidt 1982 and pv. arrhenatheri Egli and Schmidt 1982 by numerical analysis of phenotypic features and protein gel electrophoregrams. J. Phytopathol. 118:135–156
    [Google Scholar]
  47. Van den Mooter M., Swings J. 1990; Numerical analysis of 295 phenotypic features of 266 Xanthomonas strains and related strains and an improved taxonomy of the genus. Int. J. Syst. Bacteriol. 40:348–369
    [Google Scholar]
  48. Vauterin L. Unpublished data
  49. Vauterin L., Hoste B., Yang P., Alvarez A., Kersters K., Swings J. 1993; Taxonomy of the genus Xanthomonas, . 157–192 Swings J. G., Civerolo E. L. Xanthomonas Chapman & Hall; London:
    [Google Scholar]
  50. Vauterin L., Swings J., Kersters K. 1991; Grouping of Xanthomonas campestris pathovars by SDS-PAGE of proteins. J. Gen. Microbiol. 137:1677–1687
    [Google Scholar]
  51. Vauterin L., Swings J., Kersters K., Gillis M., Mew T. W., Schroth M. N., Palleroni N. J., Hildebrand D. C., Stead D. E., Civerolo E. L., Hayward A. C., Maraite H., Stall R. E., Vidaver A. K., Bradbury J. F. 1990; Towards an improved taxonomy of Xanthomonas . Int. J. Syst. Bacteriol. 40:312–316
    [Google Scholar]
  52. Vauterin L., Vantomme R., Pot B., Hoste B., Swings J., Kersters K. 1990; Taxonomic analysis of Xanthomonas campestris pv. begoniae and X. campestris pv. pelargonii by means of phytopathological, phenotypic, protein electrophoretic and DNA hybridization methods. Syst. Appl. Microbiol. 13:166–176
    [Google Scholar]
  53. Vauterin L., Van Vaerenbergh J. Unpublished data
  54. Vauterin L., Yang P., Hoste B., Pot B., Swings J., Kersters K. 1992; Taxonomy of xanthomonads from cereals and grasses based on SDS-PAGE of proteins, fatty acid analysis and DNA hybridization. J. Gen. Microbiol. 138:1467–1477
    [Google Scholar]
  55. Vauterin L., Yang P., Hoste B., Vancanneyt M., Civerolo E. L., Swings J., Kersters K. 1991; Differentiation of Xanthomonas campestris pv. citri strains by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins, fatty acid analysis, and DNA hybridization. Int. J. Syst. Bacteriol. 41:535–542
    [Google Scholar]
  56. Vera Cruz C. M., Gossele F., Kersters K., Segers P., Van den Mooter M., Swings J., De Ley J. 1984; Differentiation between Xanthomonas campestris pv. oryzae X. campestris pv. oryzicola and the ‘brown blotch’ pathogen on rice by numerical analysis of phenotypic features and protein gel electrophoregrams. J. Gen. Microbiol. 130:2983–2999
    [Google Scholar]
  57. Verniere C., Pruvost O., Civerolo E. L., Gambin O., Jacquemoud-Collet J. P., Luisetti J. 1993; Evaluation of the Biolog substrate utilization system to identify and assess metabolic variation among strains of Xanthomonas campestris pv. citri. Appl. Environ. Microbiol. 59:243–249
    [Google Scholar]
  58. Wakker J. H. 1883; Vorlaufige Mitteilungen über Hyacinthenkrankheiten. Bot. Centralbl. 14:315–317
    [Google Scholar]
  59. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., Stackebrandt E., Starr M. P., Triiper H. G. 1987; Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Int. J. Syst. Bacteriol. 37:463–464
    [Google Scholar]
  60. Wiehe P. O., Dowson W. J. 1953; A bacterial disease of cassava (Manihot utilissima) in Nyasaland. Emp. J. Exp. Agric. 21:141–143
    [Google Scholar]
  61. Yang P., De Vos P., Kersters K., Swings J. 1993; Polyamine patterns as chemotaxonomic markers for the genus Xanthomonas . Int. J. Syst. Bacteriol. 43:709–714
    [Google Scholar]
  62. Yang P., Rott P., Vauterin L., Hoste B., Baudin P., Kersters K., Swings J. 1993; Intraspecific variability of Xanthomonas albilineans . Syst. Appl. Microbiol. 16:420–426
    [Google Scholar]
  63. Yang P., Vauterin L., Vancanneyt M., Swings J., Kersters K. 1993; Application of fatty acid methyl esters for the taxonomic analysis of the genus Xanthomonas . Syst. Appl. Microbiol. 16:47–71
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-45-3-472
Loading
/content/journal/ijsem/10.1099/00207713-45-3-472
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error