1887

Abstract

The elevation of the two genotypes of to species rank as and has increased the need for reliable differentiation between the two taxa. In this study, 53 strains, including strains whose species affiliations were known as well as fresh dental plaque isolates, were subjected to a multilocus enzyme electrophoretic analysis, DNA analyses in which we used whole genomic DNA, rRNA sequences, and an oligonucleotide specific for the former genotype II () as probes, and a sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of soluble cellular proteins. All of these tests consistently separated the strains into the same two distinct groups corresponding to and confirming that the two species constitute two distinct populations of bacteria. Each of the tests used independently provided reliable identification to the species level. A previously reported heterogeneity in the pattern of human immunoglobulin A1 (IgA1) degradation was not confirmed. No differences between species were observed. All of the strains induced total degradation of IgA1 within 48 h, a property that may be a virulence factor in periodontal disease development. The enzymes responsible for IgA1 degradation were not inactivated by the physiological proteinase inhibitors α-macroglobulin and α-proteinase inhibitor.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-45-3-429
1995-07-01
2024-04-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/45/3/ijs-45-3-429.html?itemId=/content/journal/ijsem/10.1099/00207713-45-3-429&mimeType=html&fmt=ahah

References

  1. Ahl T., Reinholdt J. 1991; Detection of immunoglobulin A1 protease-induced Fabα fragments on dental plaque bacteria. Infect. Immun. 59:563–569
    [Google Scholar]
  2. Carlsson J., Herrmann B. F., Hötting J. F., Sundqvist G. K. 1984; Degradation of the human proteinase inhibitors alpha-1-antitrypsin and alpha-2-macroglobulin by Bacteroides gingivalis . Infect. Immun. 43:644–648
    [Google Scholar]
  3. Chuba P. J., Pelz K., Krekeler G., de Isele T. S., Gobel U. 1988; Synthetic oligodeoxynucleotide probes for the rapid detection of bacteria associated with human periodontitis. J. Gen. Microbiol. 134:1931–1938
    [Google Scholar]
  4. Devine D. A., Pearce M. A., Gharbia S. E., Shah H. N., Dixon R. A., Gmür R. 1994; Species-specificity of monoclonal antibodies recognizing Prevotella intermedia and Prevotella nigrescens . FEMS Microbiol. Lett. 120:99–104
    [Google Scholar]
  5. Frandsen E. V. G. 1994; Carbohydrate depletion of immunoglobulin A1 by oral species of gram-positive rods. Oral Microbiol. Immunol. 9:352–358
    [Google Scholar]
  6. Frandsen E. V. G., Reinholdt J., Kilian M. 1987; Enzymatic and antigenic characterization of immunoglobulin A1 proteases from Bacteroides and Capnocytophaga spp. Infect. Immun. 55:631–638
    [Google Scholar]
  7. Gharbia S. E., Haapasalo M., Shah H. N., Kotiranta A., Lounatmaa K., Pearce M. C., Devine D. A. 1994; Characterization of Prevotella intermedia and Prevotella nigrescens isolates from periodontic and endodontic infections. J. Periodontol. 65:56–61
    [Google Scholar]
  8. Gmür G., Guggenheim B. 1983; Antigenic heterogeneity of Bacteroides intermedius as recognized by monoclonal antibodies. Infect. Immun. 42:459–470
    [Google Scholar]
  9. Gmür R., Wyss C. 1985; Monoclonal antibodies to characterize the antigenic heterogeneity of Bacteroides intermedius, . 91–119 Macario A. J. L., Conway de Macario E. Monoclonal antibodies against bacteria, 1 Academic Press; New York:
    [Google Scholar]
  10. Haffajee A. D., Socransky S. S. 1994; Microbial etiological agents of destructive periodontal diseases. Periodontology 2000 5:78–111
    [Google Scholar]
  11. Jensen S. B., Löe H., Schiött C. R., Theilade E. 1968; Experimental gingivitis in man. IV. Vancomycin induced changes in bacterial plaque composition as related to development of gingival inflammation. J. Periodontal Res. 3:284–293
    [Google Scholar]
  12. Johnson J. L., Holdeman L. V. 1983; Bacteroides intermedius comb. nov. and descriptions of Bacteroides corporis sp. nov. and Bacteroides levii sp. nov. Int. J. Syst. Bacteriol. 33:15–25
    [Google Scholar]
  13. Kilian M. 1978; Rapid identification of Actinomycetaceae and related bacteria. J. Clin. Microbiol. 8:127–133
    [Google Scholar]
  14. Kilian M. 1981; Degradation of immunoglobulins A1, A2, and G by suspected principal periodontal pathogens. Infect. Immun. 34:757–765
    [Google Scholar]
  15. Kilian M. 1991 Haemophilus,463–470 Balows A., Hausler W. J. Jr., Herrmann K. L., Isenberg H. D., Shadomy H. J. Manual of clinical microbiology,, 5. American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  16. Laughon B. E., Syed S. A., Loesche W. J. 1982; API ZYM system for identification of Bacteroides spp., Capnocytophaga spp., and spirochetes of oral origin. J. Clin. Microbiol. 15:97–102
    [Google Scholar]
  17. Laughon B. E., Syed S. A., Loesche W. J. 1982; Rapid identification of Bacteroides gingivalis . J. Clin. Microbiol. 15:345–346
    [Google Scholar]
  18. Moore W. E. C., , and Moore L. V. H. 1994; The bacteria of periodontal diseases. Periodontology 2000 5:66–77
    [Google Scholar]
  19. Mortensen S. B., Kilian M. 1984; Purification and characterization of an immunoglobulin A1 protease from Bacteroides melaninogenicus . Infect. Immun. 45:550–557
    [Google Scholar]
  20. Murphy R. W., Sites J. W. Jr., Buth D. G., Haufler G. H. 1990; Proteins 1: isozyme electrophoresis,. 45–126 Hillis D. M., Moritz C. Molecular systematics Sinauer Associates, Inc.; Sunderland, Mass:
    [Google Scholar]
  21. Poulsen K., Hjorth P., Kilian M. 1988; Limited diversity of the immunoglobulin A1 protease gene (iga) among Haemophilus influenzae serotype b strains. Infect. Immun. 56:987–992
    [Google Scholar]
  22. Sambrook J., Fritsch E. F., Maniatis T. 1989; Molecular cloning: a laboratory manual,. , 2. Cold Spring Harbor Laboratory; Cold Spring Harbor, N.Y:
    [Google Scholar]
  23. Selander R. K., Caugant D. A., Ochman H., Musser J. M., Gilmour M. N., Whittam T. S. 1968; Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl. Environ. Microbiol. 51:873–884
    [Google Scholar]
  24. Shah H. N., Gharbia S. E. 1992; Biochemical and chemical studies on strains designated Prevotella intermedia and proposal of a new pigmented species, Prevotella nigrescens sp. nov. Int. J. Syst. Bacteriol. 42:542–546
    [Google Scholar]
  25. Slots J., Reynolds H. S. 1982; Long-wave UV light fluorescence for identification of black-pigmented Bacteroides spp. J. Clin. Microbiol. 16:1148–1151
    [Google Scholar]
  26. van Winkelhoff A. J., van Steenbergen T. J. M., de Graaff J. 1988; The role of black-pigmented Bacteroides in human oral infections. J. Clin. Periodontol. 15:145–155
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-45-3-429
Loading
/content/journal/ijsem/10.1099/00207713-45-3-429
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error