Members of the genera , and were investigated for their glucose 6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) activities by using spectrophotometric (SP) and alloenzyme elecrophoresis (AE) detection methods. When the SP and AE methods were compared, the AE method failed to detect activity in two of the six strains which exhibited G6PDH and 6PGDH activities as determined by the SP detection method. On the basis of the results of SP detection, ATCC 29147(T = type strain) (G6PDH and 6PGDH negative) is not a member of the genus as currently defined, which reflects recent 16S rRNA placement, nor do ATCC 35895, ATCC 33285, 12835, and NCTC 11632(all G6PDH and 6PGDH positive) conform to their respective genus descriptions. When these organisms were grown in prereduced peptone-yeast extract broth containing 10% (wt/vol) glucose, the amounts of glucose remaining after 5 days were less than the amounts present initially for members of the genus ATCC 25285and ) and members of the genus ATCC 25845, ATCC 33574, , and ). In addition, the glucose levels were lower after 5 days of incubation in broth media containing ATCC 25845and , but not in media containing the other members of the genus tested ( NCTC 12469, ATCC 35406, and ATCC 33277). The reductions in glucose levels were not directly related to the final pH values. Our results suggest that the descriptions of the genera , and may require emendation to reflect variability in G6PDH and 6PGDH activities and glucose utilization.


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