1887

Abstract

Members of the genera , , and were investigated for their glucose 6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) activities by using spectrophotometric (SP) and alloenzyme elecrophoresis (AE) detection methods. When the SP and AE methods were compared, the AE method failed to detect activity in two of the six strains which exhibited G6PDH and 6PGDH activities as determined by the SP detection method. On the basis of the results of SP detection, ATCC 29147(T = type strain) (G6PDH and 6PGDH negative) is not a member of the genus as currently defined, which reflects recent 16S rRNA placement, nor do ATCC 35895, ATCC 33285, 12835, and NCTC 11632(all G6PDH and 6PGDH positive) conform to their respective genus descriptions. When these organisms were grown in prereduced peptone-yeast extract broth containing 10% (wt/vol) glucose, the amounts of glucose remaining after 5 days were less than the amounts present initially for members of the genus ( ATCC 25285 and . ) and members of the genus ATCC 25845, ATCC 33574, , and ). In addition, the glucose levels were lower after 5 days of incubation in broth media containing ATCC 25845and , but not in media containing the other members of the genus tested ( NCTC 12469, ATCC 35406, and ATCC 33277). The reductions in glucose levels were not directly related to the final pH values. Our results suggest that the descriptions of the genera , and may require emendation to reflect variability in G6PDH and 6PGDH activities and glucose utilization.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-45-2-246
1995-04-01
2024-11-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/45/2/ijs-45-2-246.html?itemId=/content/journal/ijsem/10.1099/00207713-45-2-246&mimeType=html&fmt=ahah

References

  1. Cato E. P., Kelley R. W., Moore W. E. C., Holdeman L. V. 1982; Bacteroides zoogleoformans (Weinberg, Nativelle, and Prevot 1937) corrig., comb, nov.: emended description. Int. J. Syst. Bacteriol. 32:271–274
    [Google Scholar]
  2. Collins M. D., Love D. N., Karjalainen J., Kanervo A., Forsblom B., Willems A., Stubbs S., Sarkiala E., Bailey G. D., Wigney D. I., Jousim-ies-Somer H. 1994; Phylogenetic analysis of members of the genus Porphyromonas and description of Porphyromonas cangingivalis sp. nov. and Porphyromonas cansulci sp. nov. Int. J. Syst. Bacteriol. 44:674–679
    [Google Scholar]
  3. Deutsch J. 1983; Glucose-6-phosphate dehydrogenase,. 190–197 Bergmeyer H. V. Methods in enzymatic analysis 3 Verlag Chemie; Weinheim, Germany:
    [Google Scholar]
  4. Holdeman L. V., Cato E. P., Moore W. E. C. 1977; Anaerobe laboratory manual. Virginia Polytechnic Institute and State University; Blacksburg:
    [Google Scholar]
  5. Holdeman L. V., Kelley R. W., Moore W. E. C. 1984; Family I. Bacte-roidaceae Pribham 1983, 10A\. 602–662In Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  6. Love D. N., Bailey G. D., Collings S., Briscoe D. A. 1992; Description of Porphyromonas circumdentaria sp. nov. and reassignment of Bacteroides salivosus (Love, Johnson, Jones, and Calverley 1987) as Porphyromonas (Shah and Collins 1988) salivosa comb. nov. Int. J. Syst. Bacteriol. 42:434–438
    [Google Scholar]
  7. Love D. N., Johnston J. L., Jones R. F., Calverley A. 1987; Bacteroides salivosus sp. nov., an asaccharolytic, black-pigmented species from cats. Int. J. Syst. Bacteriol. 37:307–309
    [Google Scholar]
  8. Love D. N., Karjalainen J., Kanervo A., Forsblom B., Sarkiala E., Bailey G. D., Wigney D. I., Jousimies-Somer H. 1994; Porphyromonas canoris sp. nov., an asaccharolytic, black-pigmented species from the gingival sulcus of dogs. Int. J. Syst. Bacteriol. 44:204–208
    [Google Scholar]
  9. Okuda K., Kato T., Shiozu J., Takazoe I., Nakamura T. 1985; Bacteroides heparinolyticus sp. nov. isolated from humans with periodontitis. Int. J. Syst. Bacteriol. 35:438–442
    [Google Scholar]
  10. Paster B. J., Dewhirst F. E., Olson I., Fraser G. J. 1994; Phylogeny of Bacteroides, Prevotella, and Porphyromonas spp. and related bacteria. J. Bacteriol. 176:725–732
    [Google Scholar]
  11. Shah H. N., Collins M. D. 1983; A review. Genus Bacteroides. A chemotaxonomical perspective. J. Appl. Bacteriol. 55:403–416
    [Google Scholar]
  12. Shah H. N., Collins M. D. 1988; Proposal for reclassification of Bacteroides asaccharolyticus, Bacteroides gingivalis, and Bacteroides endodontalis in a new genus, Porphyromonas . Int. J. Syst. Bacteriol. 38:128–131
    [Google Scholar]
  13. Shah H. N., Collins M. D. 1989; Proposal to restrict the genus Bacteroides (Castellani and Chalmers) to Bacteroides fragilis and closely related species. Int. J. Syst. Bacteriol. 39:85–87
    [Google Scholar]
  14. Shah H. N., Collins M. D. 1990; Prevotella, a new genus to include Bacteroides melaninogenicus and related species formerly classified in the genus Bacteroides . Int. J. Syst. Bacteriol. 40:205–208
    [Google Scholar]
  15. Shah H. N., Gharbia S. E. 1992; Biochemical and chemical studies on strains designated Prevotella intermedia and proposal of a new pigmented species, Prevotella nigrescens sp. nov. Int. J. Syst. Bacteriol. 42:542–546
    [Google Scholar]
  16. Shah H. N., Williams R. A. D. 1982; Dehydrogenase patterns in the taxonomy of Bacteroides. J. Gen. Microbiol. 128:2955–2965
    [Google Scholar]
  17. Shah H. N., Williams R. A. D. 1987; Utilization of glucose and amino acids by Bacteroides intermedius and Bacteroides gingivalis . Curr. Microbiol. 15:241–246
    [Google Scholar]
  18. Staley J. T., Krieg N. R. 1984; Classification of procaryotic organisms: an overview,. 1–4In Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  19. Turner A. W., Campbell A. D., Dick A. T. 1935; Recent work on pleuropneumonia contagiosa bovum in North Queensland. Aust. Vet. J. 11:63–70
    [Google Scholar]
  20. Watabe J., Benno Y., Mitsouka T. 1983; Taxonomic study of Bacteroides oralis and related organisms and proposal of Bacteroides veroralis sp. nov. Int. J. Syst. Bacteriol. 33:57–64
    [Google Scholar]
/content/journal/ijsem/10.1099/00207713-45-2-246
Loading
/content/journal/ijsem/10.1099/00207713-45-2-246
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error