1887

Abstract

DNA-DNA hybridization studies were performed with members of the genus NCTC11632 (T = type strain) exhibited an average level of intraspecies DNA-DNA hybridization with VPB 3313 and VPB 3444 of 95% and an average level of DNA-DNA hybridization with ATCC 33141 of 81%. However, while the cat strains and monkey strain which I studied exhibited sufficient hybridization to be considered members of a single species, the tight DNA hybridization clustering and phenotypic differences suggested that the biovars isolated from cats and monkeys are distinct and can be recognized by colonial and growth characteristics, lipase activity, sorbitol utilization, proteinase patterns, and whole-cell protein profiles on sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels. As is a senior synonym of comb. nov. is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-45-1-90
1995-01-01
2024-05-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/45/1/ijs-45-1-90.html?itemId=/content/journal/ijsem/10.1099/00207713-45-1-90&mimeType=html&fmt=ahah

References

  1. Collings S., Love D. N. 1992; Further studies on some physical and biochemical characteristics of asaccharolytic pigmented Bacteroides of feline origin. J. Appl. Bacteriol 72:529–535
    [Google Scholar]
  2. Coykendall A. L., Kaczmarek F. S., Slots J. 1980; Genetic heterogeneity in Bacteroides asaccharolyticus (Holdeman and Moore 1970) and proposal of Bacteroides gingivalis sp. nov. and Bacteroides macacae (Slots and Genco) comb. nov. Int. J. Syst. Bacteriol 30:559–564
    [Google Scholar]
  3. Fournier D., Mouton C. 1993; Phenotypic characterization of human and animal biotypes within the species Porphyromonas gingivalis . Res. Microbiol 144:435–444
    [Google Scholar]
  4. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe laboratory manual, 4. Virginia Polytechnic Institute and State University; Blacksburg:
    [Google Scholar]
  5. Johnson J. L. 1985; DNA reassociation and RNA hybridization of bacterial nucleic acids. Methods Microbiol 18:33–74
    [Google Scholar]
  6. Karjalainen J., Kanervo A., Vaisanen M.-L., Forsblom B., Sarkiala E., Jousimies-Somer H. 1993; Porphyromonas-like Gram-negative rods in naturally occurring periodontitis in dogs. FEMS Immunol. Med. Microbiol 6:207–212
    [Google Scholar]
  7. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature London: 227680–685
    [Google Scholar]
  8. Lambe D. W., Ferguson K. P., Mayberry W. R. 1982; Characterization of Bacteroides gingivalis by direct fluorescent antibody staining and cellular fatty acid profiles. Can. J. Microbiol 28:367–374
    [Google Scholar]
  9. Love D. N., Bailey G. D., Collings S., Briscoe D. A. 1992; Description of Porphyromonas circumdentaria sp. nov. and reassignment of Bacteroides salivosus (Love, Johnson, Jones, and Calverley 1987) as Porphyromonas (Shah and Collins 1988) salivosa comb. nov. Int. J. Syst. Bacteriol 42:434–438
    [Google Scholar]
  10. Love D. N., Johnston J. L., Jones R. F., Calverley A. 1987; Bacteroides salivosus sp. nov., an asaccharolytic, black-pigmented species from cats. Int. J. Syst. Bacteriol 37:307–309
    [Google Scholar]
  11. Love D. N., Jones R. F., Bailey M. 1979; Clostridium villosum sp. nov. from subcutaneous abscesses in cats. Int. J. Syst. Bacteriol 29:241–244
    [Google Scholar]
  12. Love D. N., Jones R. F., Calverley A. 1984; Description of asaccharolytic black-pigmented Bacteroides strains from soft-tissue infections in cats. Int. J. Syst. Bacteriol 34:300–303
    [Google Scholar]
  13. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol 5:109–118
    [Google Scholar]
  14. Moore L. V. H. Personal communication
    [Google Scholar]
  15. North M. J., Scott K. I., Lockwood B. C. 1988; Multiple cysteine proteinase forms during the life cycle of Dictyostelium discoideum revealed by electrophoretic analysis. Biochem. J 245:261–268
    [Google Scholar]
  16. Paster B. J., Dewhirst F. E., Olson I., Fraser G. J. 1994; Phylogeny of Bacteroides, Prevotella, and Porphyromonas spp. and related bacteria. J. Bacteriol 176:725–732
    [Google Scholar]
  17. Selin Y. M., Harish B., Johnson J. L. 1983; Preparation of labeled nucleic acids (nick translation and iodination) for DNA homology and rRNA hybridization experiments. Curr. Microbiol 8:127–132
    [Google Scholar]
  18. Slots J., Genco R. J. 1980; Bacteroides melaninogenicus subsp. macacae, a new subspecies from monkey periodontopathic indigenous microflora. Int. J. Syst. Bacteriol 30:82–85
    [Google Scholar]
  19. Tereba A., McCarthy B. J. 1973; Hybridization of 125I-labeled ribonu-cleic acid. Biochemistry 12:4675–4679
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-45-1-90
Loading
/content/journal/ijsem/10.1099/00207713-45-1-90
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error