1887

Abstract

Three isolates of a previously undescribed sp. obtained from chelonids (two strains obtained from turtles and one strain obtained from a tortoise) were compared with 30 isolates obtained from Australian mammals. The microscopic appearance, the colony morphology, and most biochemical test results for the chelonid isolates were characteristic of the genus . Our isolates differed from the mammalian . isolates in a number of cultural characteristics, including faster growth at 27°C than at 37°C, formation of two hemolysis zones around colonies on blood agar at 37°C in the presence of 10% CO, poor motility, and production of a distinctive odor. The DNA restriction enzyme digestion and protein electrophoresis patterns of our strains were distinct. The electrophoretic mobilities of 11 enzymes differed from the mobilities observed with . strains. A monoclonal antibody to a surface antigen of an ovine isolate did not react with zoospores or filaments of the chelonid isolates. Biochemical differences between our isolates and . included the ability of the chelonid isolates to reduce nitrate to nitrite and the fact that the chelonid isolates exhibit collagenase activity in vitro. We propose that the chelonid isolates should be placed in a new species, . Strain W16, which was isolated from a nose scab on a snapping turtle, is the type strain; a culture of this strain has been deposited in the American Type Culture Collection as strain ATCC 51576.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-45-1-50
1995-01-01
2024-12-02
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/45/1/ijs-45-1-50.html?itemId=/content/journal/ijsem/10.1099/00207713-45-1-50&mimeType=html&fmt=ahah

References

  1. Abu-Samra M. T., Imbabi S. E., Mahgoub E. S. 1976; Dermatophilus congolensis. A bacteriological, in vitro histopathological study of natural infection in Sudanese cattle. Br. Vet. J 132:627–631
    [Google Scholar]
  2. Anver M. R., Park J. S., Rush H. G. 1976; Dermatophilosis in the marble lizard (Calotes mystaceus) Lab. Anim. Sci 26:817–823
    [Google Scholar]
  3. Ayala F. J., Powell J. R. 1972; Allozymes as diagnostic characters of sibling species of Drosophila . Proc. Natl. Acad. SciUSA 69:1094–1096
    [Google Scholar]
  4. Bauer A. W., Kirby W. M. M., Sherris J. C., Turck M. 1966; Antibiotic susceptibility testing by a standardised single disc method. Am. J. Clin. Pathol 45:493–196
    [Google Scholar]
  5. Bell S. M. 1984; Antibiotic sensitivity testing by the CDS method. New South Wales Branch of the Australian Society for Microbiology Melbourne:
    [Google Scholar]
  6. Chineme C. N., Addo P. B. 1980; Pathologic changes in lizards (Agama agama) experimentally infected with Dermatophilus congolensis . J. Wildl. Dis 16:407–412
    [Google Scholar]
  7. Cowan S. T. 1974; Cowan and Steel’s manual for the identification of medical bacteria. , 2. Cambridge University Press; London:
    [Google Scholar]
  8. Elad D., Yeruham I. 1988; In vitro sensitivity to antimicrobial agents of Dermatophilus congolensis isolated from ruminants in Israel. Isr. J. Vet. Med 44:134–136
    [Google Scholar]
  9. Ellis T. M., Masters A. M., Sutherland S. S., Carson J. M., Gregory A. R. 1993; Variation in cultural, morphological, biochemical properties and infectivity of Australian isolates of Dermatophilus congolensis . Vet. Microbiol 38:81–102
    [Google Scholar]
  10. Ellis T. M., Sutherland S. S., Davies G. 1991; Strain variation in Dermatophilus congolensis demonstrated by cross-protection studies. Vet. Microbiol 28:377–383
    [Google Scholar]
  11. Forbes C. 1989; The production and use of monoclonal antibodies in a fluorescent antibody test for detection of Dermatophilus congolensis in the environment. B. S. Honours thesis. Department of Microbiology University of Western Australia; Nedlands, Australia:
    [Google Scholar]
  12. Gordon M. A. 1964; The genus Dermatophilus . J. Bacteriol 88:509–522
    [Google Scholar]
  13. Gordon M. A. 1976; Characterization of Dermatophilus congolensis: its affinities with the Actinomycetales and differentiation from Geodermatophilus . 187–201 Lloyd D. H., and Sellers K. C. Dermatophilus infection in animals and man Academic Press; London:
    [Google Scholar]
  14. Gordon M. A. 1989; Genus Dermatophilus Van Saceghem, 1915. 2409–2410 Williams S. T., Sharpe M. E., Holt J. G. Bergey’s manual of systematic bacteriology 4 The Williams and Wilkins Co; Baltimore:
    [Google Scholar]
  15. Holzschu D. L., Phaff H. J., Tredick J. 1983; Pichiapseudocactophila, a new species of yeast occurring in necrotic tissue of columnar cacti in the North American Sonoran Desert. Can. J. Microbiol 29:1314–1322
    [Google Scholar]
  16. How S. J., Lloyd D. H. 1990; The effect of recent vaccination on the dose-response to experimental Dermatophilus congolensis infection in rabbits. J. Comp. Pathol 102:157–163
    [Google Scholar]
  17. Jordan E. O., Caldwell M. E., Reiter D. 1934; Bacterial motility. J. Bacteriol 27:165–174
    [Google Scholar]
  18. Kwapinski J. B., Simmons G. C. 1957; Serological and chemical properties of the Dermatophilus endoplasm. Antonie van Leeuwenhoek J. Microbiol 33:100–106
    [Google Scholar]
  19. Kwapinski J. B. G. 1969; Serological characteristics of particulate antigens of Dermatophilus . Can. J. Microbiol 15:1141–1144
    [Google Scholar]
  20. Lee J. I., Hampson D. J., Lymbery A. J., Härders S. J. 1993; The porcine intestinal spirochaetes: identification of new genetic groups. Vet. Microbiol 34:273–285
    [Google Scholar]
  21. Macadam I., Haalstra R. T. 1971; Bacteriology of Nigerian strains of Dermatophilus congolensis . Trop. Anim. Health Prod 3:225–231
    [Google Scholar]
  22. Maung R. T. 1963; Immunity in the tortoise Testudo ibera . J. Pathol. Bacteriol 85:51–66
    [Google Scholar]
  23. Montali R. J., Smith E. E., Davenport M., Bush M. 1975; Dermato-philosis in Australian bearded lizards. J. Am. Vet. Med. Assoc 167:533–555
    [Google Scholar]
  24. Roberts D. S. 1957; Some features of the mycotic dermatitis organism. Aust. Vet. J 33:141–143
    [Google Scholar]
  25. Roberts D. S. 1963; Properties of Dermatophilus dermatonomus zoospores in relation to the transmission of mycotic dermatitis. Aust. J. Agric. Res 14:373–385
    [Google Scholar]
  26. Roberts D. S. 1963; The influence of carbon dioxide on the growth and sporulation of Dermatophilus dermatonomus . Aust. J. Agric. Res 14:412–433
    [Google Scholar]
  27. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular cloning: a laboratory manual, 2. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, N.Y:
    [Google Scholar]
  28. Sanders A. B., How S. J., Lloyd D. H., Hill R. 1990; The effect of energy malnutrition in ruminants on experimental infection with Dermatophilus congolensis . J. Comp. Pathol 103:361–368
    [Google Scholar]
  29. Shotton D. M. 1970; Elastase. Methods Enzymol 19:113–140
    [Google Scholar]
  30. Simmons G. C., Sullivan N. D., Green P. E. 1972; Dermatophilosis in a lizard (Amphibolurus barbatus) . Aust. Vet. J 48:465–466
    [Google Scholar]
  31. Snowden J. S., Swann D. A. 1980; Vitreous structure. V. The morphology and thermal stability of vitreous collagen fibres and comparison to articular cartilage (type II) collagen. Invest. Ophthalmol. Visual Sci 19:610–618
    [Google Scholar]
  32. Sutherland S. S. Department of Agriculture; Western Australia: 1987 Personal communication
    [Google Scholar]
  33. Sutherland S. S., Ellis T. M., Edwards J. R. 1991; Evaluation of vaccines against ovine dermatophilus. Vet. Microbiol 27:91–99
    [Google Scholar]
  34. Trott D. J., Masters A. M., Carson J. M., Ellis T. M., Hampson D. J. Genetic analysis of Dermatophilus spp. using multilocus enzyme electro-phoresis. Zentralbl. Bakteriol., in press
    [Google Scholar]
  35. Willis A. T. 1960; The lipolytic activity of some clostridia. J. Pathol. Bacteriol 80:379–390
    [Google Scholar]
  36. Wilson K. 1987; Preparation of genomic DNA from bacteria. 2.4.1–2.4.5In Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. Current protocols in molecular biology 1 John Wiley and Sons; New York:
    [Google Scholar]
/content/journal/ijsem/10.1099/00207713-45-1-50
Loading
/content/journal/ijsem/10.1099/00207713-45-1-50
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error